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Original Article Upregulation of cell surface estrogen receptor alpha is associated with the mitogen-activated protein kinase/extracellular signal-regulated kinase activity and promotes autophagy maturation

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Figure

Figure 1. Immunofluorescence analysis of cell surface mERα in Triton ×100 unpermeated SH-SY5Y cells
Figure 2. Effect of E2BSA and E2 on the activation of ERK and p38 MAPK. SHSY5Y cells were cultured in the pres-ence of MPP+ (MP, 5 μg/mL), E2, E2BSA (E2, EB, 10 nM), or not
Figure 3. The time course of activation of the MAPK/ERK pathway. SHSY5Y cells were stimulated with E2BSA (EB, 10 nM), MPP+ (MP, 5 μg/mL), XCT790 (×, 5 mg/ml), 5-Hydroxytamoxifen (H, 2 mg/mL) or vehicle, and lysed at in-dicated times and analyzed for ERK ac
Figure 4. Effect of E2BSA and E2 on the modulation of autophagy. SHSY5Y cells were stimulated with E2BSA (EB, 10 nM), MPP+ (MP, 5 μg/mL), U0126 (U, 10 mmol/L) or vehicle, and lysed at 16 h, and western blot analysis of LC3-II in untreated and MPP+ treated
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