Self-Administered Training

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Self-Administered Training

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Before you begin, please have the following

documents ready:

◼

1) Chemical Hygiene Training handout (print or open

the document in a new window)

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2) Training Acknowledgement Form (must print)

◼

DO NOT save the materials as changes may be made

periodically.

◼ The training acknowledgement form must be placed in William Lee’s mailbox for proper recording. For research associates, your principal investigator may also want a copy for his/her own record.

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Laboratory Standard

(Chemical Hygiene Plan)

◼ 1990, official name “Occupational Exposures to Hazardous Chemicals in Laboratories”

◼ CCR, Title 8 Section 5191 (enforced by Cal-OSHA)

◼ Applies to all employees (paid, un-paid, students, volunteers, etc.) who use chemicals in laboratory environment

◼ Provides fundamental safety protocols and procedures

◼ Copy of the CHP is available from principal investigator, stockroom or can be viewed online:

http://www.csun.edu/ehs/chp

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Principal Investigator

(Responsibilities of )

◼

Ensure all associates comply with the CHP

◼

Ensure personal protective equipment (PPE) is

available and utilized

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Ensure SDSs are available

◼

Conduct (and document) additional lab specific

trainings when necessary

◼

Investigate laboratory accidents, spills / releases

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Enforce good personal hygiene/safety at all times

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Research Associates

(Rights of)

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Train of hazards unique to laboratory

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Availability of personal protective equipment (PPE)

◼

Knowledge of emergency equipment or procedures

(i.e. eyewash, shower, spills)

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Aware of hazards and symptoms of exposure to

chemicals (from SDS)

◼

Follow other safe work practices (SOPs) & basic lab

rules as instructed by principal investigator

◼

Practice good personal hygiene (ex. wash hands

frequently, no eating/drinking in the lab)

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Personal Protective Equipment

Eye Protective Wear

Indirect Ventilation (or closed ventilation) Comply with ANSI Z87.1-

2015, D3 marking.

Provide a tight seal around the eyes.

Your EYES are PRICELESS. Wear safety goggles at all times. It is for YOUR protection.

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Personal Protective Equipment

Glove Selection and Compatibility

◼ Base material selection on each manufacturer’s chemical compatibility and resistance guide. Inspect for tears/holes prior to use

◼ Change gloves regularly (recommendation: 1 pair every 1 to 2 hours)

◼ Stockroom provides nitrile gloves; Principal Investigator is responsible for the purchase of other types of gloves (s/he will determine the needs)

Neoprene Nitrile

Natural Rubber

Vinyl

Handout Page 1

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Inappropriate Appropriate

Personal Protective Equipment

Proper Attire

Not allowed Must wear close- toed shoes

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Personal Protective Equipment

Department Policy

Safety Goggles¹ Safety Glasses

Safety goggles must be worn when:

◼ Handling chemicals that are hazardous (i.e. flammable, toxic, corrosive, etc.)

◼ Handling any liquid chemicals

Safety glasses may be worn in place of safety goggles when all of the following conditions are met:

◼ Explicitly allowed by P.I.

◼ Handling nonreactive/nontoxic solids / aqueous solutions

◼ Peripheral vision is critical to safe operation

◼ No other is handling of hazardous materials in area or in line of sight

Gloves ◼ When handling chemicals that are hazardous (i.e. toxic, corrosive, poison, etc.), regardless of physical state

◼ When instructed by P.I.

Lab Coat² ◼ Highly recommended to protect exposed skin from direct chemical contact

◼ When instructed by P.I.

Shoes³ Must wear closed-toe shoes at all time

Availability Safety googles and gloves are available from the Stockroom; P.I. may have extra lab coats to check out

1 = approved chemical resistant goggles; 2 = 100% cotton or cotton blend materials; 3 = sneakers/casual dress shoes

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“Buddy System”

Working alone is strongly discouraged.

Follow your principal investigator’s advices.

If none, use the following “buddy system” hierarchy:

1) Buddy works in the same lab (best)

2) Buddy works in a lab few doors down

3) Buddy works on another floor or building (least)

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Safety Equipment

Emergency Eyewash / Shower

Run some water through the openings first.

Retract (spread) eyelid(s) and flush for 15 minutes.

- go to the Student Health Center for further check-up (escorted by another person)

Flushed monthly by Dept. Safety Coordinator.

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Position sash at the arrow; Work at least 6 inches inside the hood.

Safety Equipment

Fume Hood

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Safety Equipment

Fume Hood

Used when

1) working with compounds that have an offensive odor 2) chemical vapors generated could cause a fire hazard

3) handling chemicals that have significant inhalation hazards

When the fume hood is properly used and is functional, odors emanate from the chemicals in the lab should be minimal or

non-existence.

If chemical odor is detected, stop working immediately and determine the source of the odor. Do not continue to work until the source of odor has been determined and mitigated. Don’t endanger yourself by becoming a human filtering machine.

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Safety Equipment

Fire Extinguisher

At least one fire extinguisher per lab Multipurpose for Class A, B & C fire

Contains dry chemical (monoammonium phosphate) under nitrogen.

P – pull the pin

A – aim at the base of the fire S – squeeze the handle

S – sweep side to side

Do not use it on a person (inhalation hazard); instead, use the safety shower, or (next slide)…

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Clothes on Fire

STOP, DROP, ROLL

Stop - The fire victim must ceasing any movement which may fan the flames or hamper those attempting to put the fire out.

Drop - ‘Drop' to the ground, lying down if possible, covering their face with their hands to avoid facial injury.

Roll - Victim must roll on the ground in an effort to extinguish the fire by

depriving it of oxygen. If the victim is on a rug or one is nearby, they can roll the rug around themselves to further extinguish the flame.

The effectiveness of stop, drop and roll may be further enhanced by combining

it with other firefighting techniques, including dousing with water, or fire

beating.

Courtesy of RoSPA

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Located in a labeled drawer or a labeled container

Treat your own injuries.

If the injury is serious, have another person escort the injured to the Student Health Center for further check-up/treatment.

Safety Equipment

First Aid Kit

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Glassware must be free of chemicals;

NO regular trash, biohazard materials, or mercury thermometers Sweep and

discard all cracked or

broken glassware in the red can in

the lab. Never carry them outside

the lab.

Safety Equipment

Broken Glassware Receptacle

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Safety Equipment

Translucent Container for Chemical Contaminated

For: syringes, needles,

razor blades

Never recap a needle. Needle

stick!!!

If safely to do so: remove any

residual chemicals by rinsing

the syringe/needle with a

proven solvent (haz. waste).

Never carry them outside the

lab.

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Needles, syringes, razor blades,

glass Pasteur pipets, broken

glass etc.

Never recap a needle. Needle

stick!!!

Never carry them outside the

lab.

Safety Equipment

Red Container for Biohazard Contaminated

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Safety Equipment

Non-Sharps Biohazard Materials

Place the bag inside a rigid container (e.g. a trash can) --- ONLY objects that will NOT puncture the plastic bags (e.g. gloves, petri dishes,

ampules, cotton swabs, etc.) --- NO needles / syringes --- NO broken glassware --- NO regular trash

Fill the bag no more than ½ full.

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Goose neck

Afterwards, place the bag in the secondary containment for pick up.

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Safety Equipment

Spill Kit

Safety goggles

All purpose sorbent

Disposable gloves

Collection bag Broom and

dust pan

minimum content

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◼ Safety Concerns:

◼ Inhalation of volatile vapors (may cause nausea, dizziness, eye irritation or other harmful health effects).

◼ Violation of certain Federal or State laws.

◼ Recommendation:

◼ Partial Failure: move operation to a working fume hood. Notify Stockroom.

◼ Total Failure: Stop working and evacuate. Notify Principal Investigator and Stockroom. Pull the sash down to the lowest position. Close all doors and windows.

◼ Fume hood repair:

◼ May take hours (or less) if problem is minor (e.g. broken fan belt)

◼ May take days if problem is major (e.g. broken motor or broken shaft)

Emergency Procedures

Fume Hood Failure – What To Do?

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Emergency Procedures

Chemical Spills

◼ Simple spills (of 4 liters or less)

◼ Capable: can easily be cleaned up (i.e. acid/base spill)

◼ Knowledge: no potential risk of health hazards (fire, explosion, or chemical exposure)

◼ Equipment: can use Spill Kit mentioned earlier to perform clean up

◼ Complex spills (of 4 liters or more)

◼ Contain the spill if possible

◼ Notify Principal Investigator and others in the area, evacuate the spilled area and close the door(s)

◼ Notify Office (3381/2) and Stockroom (3371/2)

◼ After business hours, call police (2111 or 911 from campus phone)

No clean up is required. However, you are required to notify proper authority and others in the area for safety reasons. Also, leave a note

on the door with description (what, when, how much, etc.).

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Chemical Labeling

Fire Diamond

NFPA 704

Handout Page 2

Fire diamond is based on the

chemicals stored in a lab. There is also a fire

diamond for the building. Higher the number,

higher the

danger/hazard.

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Safety Data Sheets (SDS)

◼

Need SDS for each chemical used

◼

Look up the SDS on the internet

Principal Investigator must give you ample time to research and read the materials.

Should keep hardcopy in the lab if chemicals:

- stored are high in volume.

- are toxic (TLV or PEL <50ppm).

Handout Pages 3-7

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It may be a fume hood, parts or entire laboratory. Majority of the research labs have designated the entire lab as the Designated Area;

materials can be stored anywhere in the lab.

“An area which may be used for work with select carcinogens, reproductive toxins or substances that have a high degree of

acute toxicity.”

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Compressed Gas / Liquid Cylinders

Compressed Gas Cylinders

◼

Move cylinders using designated carts equipped with

straps/chains

◼

Cylinders are capped and lines purged when in storage or

will not be in use for extended period

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Compressed Gas / Liquid Cylinders

Compressed Gas Cylinders

You may use stockroom’s cart or your own cart for transportation. Make sure the cylinder is secured with the chain/bar.

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A status (i.e. empty / in service / full) tag is attached.

Attached to cylinder rack and secured with chains / bars.

Compressed Gas / Liquid Cylinders

Compressed Gas Cylinders

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Empty – next to Citrus Hall elevator Full – in Eucalyptus Hall 2323

Compressed Gas / Liquid Cylinders

Compressed Gas Cylinders

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Liquid Nitrogen

◼ When returned to the gaseous state, can displace

oxygen from the air and can create an oxygen-deficient atmosphere under the right conditions.

◼ High concentrations of nitrogen reduce the breathable oxygen in the air.

◼ 1 cubic foot of liquid nitrogen will expand to 696 cubic feet of 100% gaseous nitrogen at 70° F

◼ Oxygen deficiency can lead to asphyxiation:

– Asphyxia develops slowly as the oxygen content of air is gradually reduced from 21%.

– Oxygen concentration below 19.5% is considered an oxygen deficient environment

– Safe Range: ≥19.5% and ≤23%

– The victim will not be aware of a problem and generally will not recognize the symptoms of gradual asphyxia from decreasing oxygen levels.

Boiling Point: -196 ^oC.

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Compressed Gas / Liquid Cylinders

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Compressed Gas / Liquid Cylinders

Liquid Nitrogen Cylinders

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Compressed Gas / Liquid Cylinders

Liquid Nitrogen Cylinders

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Compressed Gas / Liquid Cylinders

Liquid Nitrogen Cylinders

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Hazardous Chemicals Storage

Store no more than 10 gallons of

flammables in the open space in the lab (all excess should be stored in the

approved flammable cabinet).

Never store chemicals alphabetically.

Store chemicals within compatible

groups.

Handout Pages 8-11

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Hazardous Waste and Disposal

Hazardous Waste Determination

◼

In CA: presumptive waste --

all waste is hazardous unless shown otherwise by regulation.

◼

What is hazardous waste?

EPA Definition:

A material is a hazardous waste if due to its quantity,

concentration, physical, chemical or infectious characteristics it possesses a substantial present, or potential hazard to

human health and the environment and has no known use.

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Hazardous Waste and Disposal

Hazardous Waste Storage and Disposal

◼ Never intentionally release into sewer, trash, ground or air

and harms the

environment.

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Hazardous Waste and Disposal

Hazardous Waste Storage and Disposal

◼

Ensure waste stream is compatible with the

container

◼

Do not mix incompatible chemicals

◼

Container must be capped/closed (not over tighten)

unless waste is being added or removed

◼

Leave a minimum of 2” void space or below the

neck

◼

Place all glass/metal liquid waste containers in a

secondary container (and segregated by

compatibility of the contents)

◼

Store in a designated area in the lab

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minimum 2” void space

DO NOT Top-Off

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A funnel is not a cap

Keep container

capped unless adding waste to it

_.

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Hazardous Waste and Disposal

Hazardous Waste Labeling

Circle all that apply. Circle all that apply.

Must complete: Fill in the date when first drop is collected.

NO Organic solvents, EtOh, CH₂Cl_2,HCl,

chromatography solvents OR waste. Instead, write non-halogenated organic solvents OR halogenated organic solvents contains aqueous basic solution, or any compatible waste combinations (next slide for more).

Must complete. NO abbreviations, formulas, structures, etc.

Instead, spell out the names fully.

H A Z A R D O U S W A S T E

STATE AND FEDERAL LAW PROHIBIT IMPROPER DISPOSAL Item #: _______________ Dept: Chemistry

Workplace Accumulation ________________________ Accumulation Start Date ________________________

Chemical Name or Description

_________________________________________________________________ __________________________________________________________________ __________________________________________________________________ __________________________________________________________________ __________________________________________________________________

Physical State (circle all that apply) Solid Liquid Gas Hazardous Properties (circle all that apply)

Flammable Reactive Toxic Corrosive Explosive Acute Poison Other

California State University, Northridge 18111 Nordhoff Street

Northridge, CA 91330-8284 (818) 677-2401

Leave Blank Leave Blank

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◼ Segregate waste into following classification (minimum)

Hazardous Waste and Disposal

Hazardous Waste Storage and Disposal

◼ Non-halogenated organic solvents

◼ Halogenated organic solvents

◼ Mercury containing solutions

◼ Any chemicals with

toxicity <50ppm must be listed

◼ Acidic solution containing metal compounds

◼ Basic solution containing metal compounds

◼ Or any currently approved waste descriptions in

individual research lab

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Hazardous Waste and Disposal

Hazardous Waste Storage and Disposal

Request a pick-up every 70 days or less (principal investigator will receive reminder e-mail)

Fill out Hazardous Waste Pick-up Request Form on-line at:

http://www.csun.edu/~chemsafe (follow instructions)

Waste is only picked up between Tuesday and Thursday.

*** Improperly labeled container (missing Accumulation Date, or missing Chemical name / Description will be refused for pick-up. ***

Must submit a NEW request after the corrections have been made.

If you need empty containers prior to pick up, please see Stockroom personnel (EH 2320).

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Hazardous Waste and Disposal

Dispose of Empty Container

What is empty?

1) All material inside is removed, to the best extent

2) For liquids, all materials removed by practical means (pumping, aspirating and draining)

3) For acute or extremely hazardous, triple rinse or other proven methods capable of removing the materials (rinsate should be managed as

hazardous waste)

Disposal Procedure:

1) Leave empty solvent container (with lid off) in the fume hood for a minimum of 24 hours

2) Remove or deface the identifying label

3) Discard the container in the blue trash dumpster (between EH and CS)

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Hazardous Waste and Disposal

Dispose of Empty Container

Remove or deface the identifying label prior to disposal.

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Hazardous Waste and Disposal

Disposal of Empty Container

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Hazardous Waste and Disposal

Disposal of Empty Container

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Biological Safety

Biosafety Level

Laboratory Type Laboratory Practices Safety Equipment

Basic – Biosafety Level 1

Basic teaching, research

GMT None, open bench work

Basic – Biosafety Level 2

Primary health

services; diagnostic services, research

GMT plus protective clothing, biohazard sign

Open bench plus BSC for potential aerosols

Containment – Biosafety level 3

Special diagnostic services, research

As Level 2 plus special clothing, controlled access, directional airflow

BSC and/or other primary devices for all activities

Maximum containment – Biosafety Level 4

Dangerous pathogen units

As Level 3 plus

airlock entry, shower exit, special waste disposal

Class III BSC, or positive pressure suits in conjunction with Class II BSCs, double- ended autoclave (through the wall), filtered air

BSC = biological safety cabinet; GMT = good microbiological techniques Handout Pages 12-13