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Simple, Sensitive and Accurate Multiplex Detection of Clinically Important Melanoma DNA Mutations in Circulating Tumour DNA with SERS Nanotags

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Academic year: 2020

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Figure

Figure 1. Conceptual schematic of the multiplex PCR/SERS assay and SERS nanotags. (A) Multiplex mutation specific primers were used to amplify tumour DNA
Figure 2. Specific multiplex detection by SERS. Specific detection of individual mutant (MT) targets: (A) BRAF V600E, (B) cKIT L576P and (C) NRAS Q61K
Figure 4. Accurate genotyping of melanoma cell lines. (A-C): typical SERS spectra for three melanoma cell lines
Figure 5. Accurate genotyping of ctDNA samples. (A-E): Typical SERS spectra for 5 ctDNA samples
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