ANS Reproductive Physiology of Domestic Animals (Spring 2015)

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ANS 431 - Reproductive Physiology of Domestic Animals

(Spring 2015)

Instructor:

Dr. Eduardo L. Gastal, DVM, MS, PhD

Room: AG 129; Phone: 453-1774; E-mail: egastal@siu.edu

Office hours: MWF 11-12 a.m.; MF 4-6 p.m. or by appointment.

Teaching Assistants (TAs):

Mr. Ghassan Ishak, DVM, MS, PhD student ghassan@siu.edu

Office hours: by appointment. (Primary Contact for any queries)

Time:

Lecture: 10:00 to 10:50 a.m., MWF at room AG 170

Labs: 8:00 to 9:50 a.m. (Section 1), 10:00 to 11:50 a.m. (Section 2) Thursday at AG 114. Attendance is mandatory in laboratories. Lab coats for labs in AG 114, and adequate barn clothes for labs at the farms are mandatory.

Course Learning Objectives:

The objective of this course is to allow the students to know and understand the following: general anatomy and histology of the male and female reproductive systems; endocrinology of the estrous cycle, pregnancy, and lactation; estrous cycle and gestation in farm animals; functions of the cells of the ovary and testis; basics of oocyte and spermatozoa maturation; transport of gametes through the male and female reproductive systems; steps involved in fertilization; basic events of embryo and fetal development; basics of maternal recognition of pregnancy, implantation, and placentation; events of parturition; events of courtship and mating in farm animals; general causes and examples of reproductive failure in farm animals; reproductive peculiarities and management of cattle, sheep, horses, swine, and poultry; basics of reproductive-assisted biotechnologies including AI, estrus synchronization, superovulation, embryo transfer, IVF, sperm and embryo sexing, transgenics, and reproductive immunization.

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Every lecture handout will have several questions to serve as a study guide for the students to prepare for the quizzes and exams. Students are encouraged to answer the questions after every lecture day and before the next lecture. Study guides are going to be do every week. Please see below for more details.

Hours of Study Required for this Course:

At least 4 hours per week.

Textbook:

Senger P.L. 2003, 2005, 2012. Pathways to Pregnancy and Parturition. 2

nd

or 3rd revised

edition. Current Conceptions, Inc.

Other References:

Hafez B., Hafez E.S.E. 2000. Reproduction in Farm Animals. 7th edition. Lippincott Williams & Wilkins. Knobil E., Neill J.D. 1994. The Physiology of Reproduction. 2nd edition. Raven Press.

Knobil E., Neill J.D. 1998. Encyclopedia of Reproduction. Academic Press. Vols. 1-4.

Ginther O.J. 1992. Reproductive Biology of the Mare, Basic and Applied Aspects, 2nd edition. Cross Plains, WI: Equiservices Publishing.

Ginther O.J. 1995. Ultrasonic Imaging and Animal Reproduction: Book 2, Horses. Cross Plains, WI: Equiservices Publishing.

Ginther O.J. 1998. Ultrasonic Imaging and Animal Reproduction: Book 3, Cattle. Cross Plains, WI: Equiservices Publishing.

Grading:

Study guides (“quizzes”):

Study guides will be due weekly and are take-home format. Each PowerPoint lecture will contain a final slide with objective, straight forward questions highlighting important concepts from that lecture. Students will be required to answer only 5 questions from each lecture and will turn in the answers every Monday from all lectures covered up through the previous Friday. Since each week will consist of 2 to 3 lectures, students should expect to be responsible for answering 10 to 15 questions per week. Of these, 3 questions will be chosen at random and graded to account for the weekly quiz grade. Once the quizzes are graded, the answers to all questions will be posted on Blackboard to serve as a study guide.

Exams:

Exams will be given during the lecture period in room AG 170. Students may come early and remain later than the scheduled lecture period. Contact instructor for specifics. Exam formats may vary throughout the

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semester, but students should expect to see multiple choice, true/false, short answer and essay questions (this can also be expected of the quizzes). Students should review their missed questions and return exam on the following class. Students will miss points if exams are NOT returned on time!

Four Mid-terms (Exam 1, Exam 2, Exam 3) and a Final Exam will be given in the course. Make-up exams will be essay or oral at the instructors' discretion. 20% will be taken off the top of any make-ups given to students who do not notify an instructor before the regularly scheduled exam. The Final may be 50% comprehensive. The final will only be given at the scheduled time - no make-ups or early exams. Students that do not take the final will be given "0" on the final or an incomplete depending on the circumstances determined by the instructor.

Attendance:

Attendance is important and will be considered mandatory in both lab and lecture periods and will be factored into the student’s grade, accounting for 10%.

Grading Breakdown: 15% Laboratory and Attendance A=90 to 100% 20% Study guides (“quizzes”): B=80 to 89%

50% 3 Exams C=70 to 79%

15% Final Exam D=60 to 69%

F=<60% 100% Total

Changes to these grade items and points allocated are at the discretion of the instructor. If grades are applied to a curve the instructor reserve the right to lower the curve as appropriate. The curve will not be raised. Graduate students will not be included in the undergraduate student curve.

Academic Honesty:The academic honesty policy will follow the Student Conduct Code.

ADA Accommodation: In keeping the goal of the implementation of the Americans with Disabilities Act (ADA), all students for whom the act applies should notify the instructor no later than the second session of the course so that arrangements can be made for accommodations to meet your educational needs and maximize learning.

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Tentative Schedule – Spring 2015

Week

Date

Title

Reading in Text

1 Jan 21 Lec 1 – Introduction, course structure, historical perspectives

Jan 22 Lab 1

Generalities about the course and introduction to each other

Jan 23 Lec 2a – Female reproductive tract anatomy Chapter 1, 2 2 Jan 26 Lec 2b – cont. Female reproductive tract anatomy

Jan 28 Lec 3a– Female comparative anatomy Jan 29 Lab 2

Female anatomy

Jan 30 Lec 3b– cont. Comparative female anatomy, historical perspectives

3 Feb 2 Lec 4 – Male reproductive tract anatomy Chapter 3 Feb 4 Lec 5 – Comparative male reproductive tract Chapter 3 Feb 5 Lab 3

Female anatomy, follicular aspiration from tracts, GC, oocytes

Feb 6 Lec 6a – Embryogenesis, sexual/genital development Chapter 4 of the male and female

4 Feb 9 Lec 6b – Embryogenesis, sexual/genital development Chapter 4 of the male and female

Feb 11 Lec 6c – Sexual differentiation Chapter 4

Feb 12 Lab 4

Male anatomy, epididymal sperm

Feb 13 REVIEW SESSION

5 Feb 16 EXAM 1

Feb 18 Lec 7a – Endocrinology of reproduction Chapter 5 Feb 19 Lab 5

AI practice with the genital tracts

Feb 20 Lec 7b – Endocrinology of reproduction Chapter 5 6 Feb 23 Lec 7c – Endocrinology of reproduction-Mechanism of hormone action Chapter 5

Feb 25 Lec 8a – Puberty Chapter 6

Feb 26 Lab 6

Gynecologic exam and AI

cows

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7 Mar 2 Lec 9a – Reproductive cycles in the female Chapter 7 Mar 4 Lec 9b – cont. Reproductive cycles in the female Chapter 7 Mar 5 Lab 7 –

Male exam, testis size, and semen collection

bull

Mar 6 Lec 10a – Follicular phase of the estrous cycle, follicular waves, Chapter 8 oogenesis, ovulation

8 Mar 7 -15 – SPRING BREAK

9 Mar 16 Lec 10b – Follicular phase of the estrous cycle, follicular waves, Chapter 8 oogenesis, ovulation

Mar 18 REVIEW SESSION

Mar 19 Lab 8

Gynecologic exam and AI

mares

Mar 20 Lec 11a Luteal phase of the estrous cycle, the menstrual cycle, and Chapter 9 estrus synchronization

10 Mar 23 EXAM 2

Mar 25 Lec 11b Luteal phase of the estrous cycle, the menstrual cycle, and Chapter 9 estrus synchronization

Mar 26 Lab 9

AI and estrus detection

sows

Mar 27 Lec 11c Sexual Behavior Chapter 11

11 Mar 30 Lec 12 Applied Reproductive behavior of the male, semen collection and processing April 1 Lec 13 – Artificial insemination

April 2 Lab 10

Male exam, testis size, and semen collection

stallion

April 3 Lec 14a – Spermatogenesis Chapter 10

12 April 6 Lec 14b – Spermatogenesis Chapter 10

April 8 Lec 14c – Sperm, epididymis, ejaculation, semen Chapter 10 April 9 Lab 11

Pregnancy detection

(

genital tracts in lab

)

April 10 Lec 15 – Gamete transport Chapter 12

13 April 13 REVIEW SESSION April 15 EXAM 3

April 16 Lab 12

Pregnancy check in

cows

(

rectal palpation + ultrasonography

)

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14 April 20 Lec 16b – Fertilization, embryo development Chapter 12,13 April 22 Lec 16c – Fertilization, embryo development Chapter 12,13 April 23 Lab 13

Ultrasonographic examination +

embryo transfer (mare)

April 24 Lec 17a – Placentation and maternal recognition of pregnancy Chapter 13, 14 15 April 27 Lec 17b – Placentation and maternal recognition of pregnancy Chapter 13, 14 April 29 Lec 18a – Pregnancy loss and parturition Chapter 14,15 April 30 Lab 14

Ovum pick-up – cow

May 1 Lec 18b – Pregnancy loss and parturition Chapter 14,15 16 May 4 Lec 19 – Biotechnologies applied to animal reproduction

May 6 REVIEW SESSION

Wed , May 13 10:15 – 12:15 p.m

. FINAL EXAM

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