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Curcumin-loaded redox response of self-assembled micelles for enhanced antitumor and anti-inflammation efficacy

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Figure 1 The characteristics of different micelles.Notes: (A) FTIR spectra of C16-ss-cOOh, c16-ss-cs, and c16-SS-CS-mPEG. (B) 1h NMr spectra of c16-SS-CS-mPEG polymer in D2O (ppm). TEM micrographs of (C) C16-ss-CS-mPEG@Cur  micelles  and  (D)  C16-CC-CS-mPEG@Cur  micelles.  The  size  distribution  by  DLS  of  (E)  C16-SS-CS-mPEG@Cur  micelles  and  C16-CC-CS-mPEG@Cur micelles.Abbreviations:  FTIR,  Fourier  transform-infrared  spectrometry;  C16-SS-CS-mPEG,  monomethoxy-poly(ethylene  glycol)-chitosan-S-S-hexadecyl;  C16-CC-CS-mPEG, monomethoxy-poly(ethylene glycol)-chitosan-C-C-hexadecyl; Cur, curcumin; 1h NMr, 1H nuclear magnetic resonance; TEM, transmission electron microscopy.
Figure 2 In vitro drug release from micelles.Notes: (A) Calibration curves of Cur at concentrations of 0.1–2.0 μg ml−1. Reduction-triggered release of Cur from (B) C16-SS-CS-mPEG@Cur micelles and (C) Cur-loaded c16-CC-CS-mPEG@Cur micelles in PBS (10 mM, pH 7.4) with, or without, 10 mM DTT. Data are shown as mean ± SD (n=3).Abbreviations: c16-SS-CS-mPEG,  monomethoxy-poly(ethylene  glycol)-chitosan-S-S-hexadecyl;  C16-CC-CS-mPEG,  monomethoxy-poly(ethylene  glycol)-chitosan-C-C-hexadecyl; Cur, curcumin; PBS, phosphate-buffered saline; SD, standard deviation.
Figure 3 Effect of endocytosis inhibitors on the uptake of different micelles.Note:  Effect  of  endocytosis  inhibitors  on  the  uptake  of  mPEG-S-S-C16@Cur  micelles  and  mPEG-C-C-C16@Cur  micelles  on  MCF-7  cells  using  CLSM  (A)  and  FCM (B) analyses.Abbreviations: c16-SS-CS-mPEG, monomethoxy-poly(ethylene glycol)-chitosan-S-S-hexadecyl; C16-CC-CS-mPEG, monomethoxy-poly(ethylene glycol)-chitosan-C-C-hexa-decyl; Cur, curcumin; CLSM, confocal laser scanning microscope; FCM, flow cytometry; MFI, mean fluorescence intensity; MBCD, methyl-β-cyclodextrin.
Figure 4 (A and B)  CLSM  images  of  MCF-7  cells  after  treatment  with  PBS,  Cur,  C16-CC-CS-mPEG@Cur,  and  C16-SS-CS-mPEG@Cur  for  2,  12,  24,  and  48  h  and corresponding quantitative analysis of Cur fluorescence intensity.Note: The control exhibited no fluorescence.Abbreviations: CLSM, confocal laser scanning microscope; PBS, phosphate-buffered saline; C16-SS-CS-mPEG, monomethoxy-poly(ethylene glycol)-chitosan-S-S-hexadecyl; 16-CC-CS-mPEG, monomethoxy-poly(ethylene glycol)-chitosan-C-C-hexadecyl; Cur, curcumin.
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