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Label-Free Time-Gated Luminescent Detection Method for the Nucleotides with Varying Phosphate Content

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Figure

Fig. 1 Apparent mode of action of the label-free nucleotide sensor. A) Tb(III)-N1 forms a luminescent Probe 1 complex with antenna 1 in neutral or acidic aqueous solution
Table 1 Summary of the binding properties of the Probe 1 to different phosphate-containing anions.a
Fig. 2 Time-gated luminescence response curves of Probe 1 with different phosphate-containing molecules
Fig. 3 Time-gated luminescence monitoring of the K-Ras GTPase activities. A) GTPase cycle of GDP-GTP exchange (SOScat) and GTP hydrolysis (p120RasGAP or NF1) with wild-type K-Ras reduces free GTP concentration increasing Probe 1 time-gated Tb(III)-luminesc
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