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Homeobox genes from clusters A and B demonstrate characteristics of temporal colinearity and differential restrictions in spatial expression domains in the branching mouse lung

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Academic year: 2020

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Figure

Fig. 2. Temporal expression patternsof}Jg of lung mRNMrack) HoxB genesduringlung development.(a1, b1, c1 and d1' Phospho-Imagmgof the 68 kb Hoxb-J (30 Jigof rota/lungRNMrackJ,2
Fig. 3. In situE'1.5hybridizationanalysesof HoxAspatialexpressionin themouselung. (e) epithelium.(m) mesenchyme.(a) Light and darkfield Illuminationsof a Hoxa-lantisenseprobe
TABLE 2of PCR cyclesdllutJOn; (iv) IS the(I) IS 1: rhe,: 1 dilutIOn; 10 dilution.
Fig. 6. Ouantitationoftranscriptsamplifiedareafternumberat therespectivelysize Hoxa-1in distalbudversusinterbudregions.(DB)distalbud.(lB)interbud.la) Rep-resentativephospho-imageofthe 711bpand509bpHo:\a-1and549bpvimentinPCR productsSouthernblotanalysis.Numbers10-35indicatetheofPCRcycles.(I) OBIHo:\a-l;(ii) IBIHoxa-l;(iii)OBIVlmentln(the minorbandsseen 30 and 35 cycle intervalscorrespondto thepredictedfor thevimentlnPCRoligonucleotides);(iv) IBIvimentln.(b) PSL valuesof eachproductin eachregion shown on semllogarithmicgraphs
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