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Substitution of adeno associated virus Rep protein binding and nicking sites with human Chromosome 19 sequences

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Figure

Figure 1 Structure of the wild-type AAV2 and modified ITR. (A) The AAV2 ITR is composed of seven regions, a, a’, b, b’, c, c’ and d, and forms anextensive secondary structure
Figure 2 Packaging comparisons between the wild-type andmodified viruses. (A) Dot blot analysis of 3-fold serial dilutions ofnuclease-resistant AAV2 DNA made using pSub201(-) (wt), pVM108(108) pVM112 (112) or pVM141 (141)
Figure 3 Viral DNA replicationportion of pSub201. (D) Southern blot of genomic DNA from cells cotransfected with an adenovirus helper plasmid and pVM108
Figure 4 Site-specific integration atfrom thegene primer set. The templates were genomic DNA isolated from HeLa cells infected with the indicated volumes of AAV2-containingsupernatants produced using pSub201(-) (wt), pVM108 (108) or pVM113 (113)
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