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Dynamic visualization of transcription and RNA subcellular localization in zebrafish

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Academic year: 2020

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Figure

Fig. 1. Transgenic zebrafish lines expressing NLS-tdMCP-eGFP can be used to detect transcriptsembryos at the sphere stage shows that cytoplasmic puncta arevisible only in cells expressing the Cherry reporter
Fig. 2. MS2-labeling reveals the onset of transcription in zebrafishembryos. (A) Immediately after fertilization, the RNA present is exclusivelycomprised of maternal products
Fig. 3. MS2-tagged nanos3the cytoplasm of PGCs.imaging of 3′UTR is detected by MCP-GFP in (A) Schematic depicting injection and cherry-24xMBS-nanos3 3′UTR RNA
Fig. 4. MS2-tagged nos3′utr colocalizeswith endogenous nanos3 and a subset of

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