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High resolution melting analysis for the detection of EMS induced mutations in wheat SBEIIa genes

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Figure

Figure 1 Design and testing of primers for first round PCRamplicons. ForSBEIIa-B. a) Electrophoretic profile of the PCR products obtained from Langdon (1),Langdon 2D(2A) (2), Langdon 2D(2B) (3) by using homoeoallele specific primer pairs
Figure 2 High Resolution Melting analysis of second round PCR products of 96 2-fold pooled samples
Table 3 Mutations affecting enzyme functionality aspredicted by PARSE-SNP application.
Figure 5a clearly shows a drastic decrease of SBEIIa-Alevel due to both the splicing and non sense mutations,respectively, on the expression of the genes
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