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Upregulated expression of indoleamine 2, 3-dioxygenase in CHO cells induces apoptosis of competent T cells and increases proportion of Treg cells

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Figure

Figure 1 Identification of IDO transfected CHO cells. (A) Identification of recombinant plasmid pIRES2-EGFP-IDO by restriction enzymeanalysis
Figure 2 Effect of IDOFACS scatter plots of apoptotic CD3cells. (C) Representative FACS scatter plots of apoptotic CD3cells in the early process of apoptosis; P5 represents the total population of apoptotic CD3+T cells) (E) Relative percentages of apoptoti
Figure 3 Inductive effect of CHO cells with IDO transfection on Tregsdifferences were statistically significant (region represents CD4average (%) ± SD from 3 independent experiments
Figure 4 Foxp3 expression in T cells after co-culture was detected by RT-PCR, Real-time PCR or Western blotco-culture with CHO/EGFP cells; or 7 days after co-culture with IDOmedium, or from the T cells co-cultured with IDO gene-transfected CHO cells, or fr

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