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Comparison and optimization of CRISPR/dCas9/gRNA genome labeling systems for live cell imaging

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Figure

Fig. 1 Schematic views of the different CRISPR/dCas9 genome-labelingsystems. In the SunTag system, nuclease-deficient CRISPR/Cas9 (dCas9)protein is fused with a 24X repeating GCN4 peptide array, which couldrecruit multiple copies of scFv-GFP, thereby enabl
Fig. 2 Comparison of different dCas9/gRNA systems for labeling human telomeres.the negative controls (with control gRNA and without gRNA) for different CRISPR/dCas9 labeling systems.dCas9/gRNA foci in all GFP positive cells (control gRNA, and without gRNA
Fig. 3 Comparison of different dCas9/gRNA systems for labeling the endogenous human MUC4 genomic locus.bars(formation efficiency in different dCas9/gRNA labeling systems (measured as % of GFP-positive cells,MUC4 a A gRNA targeting the human gene was transf
Fig. 4 (See legend on next page.)
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