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Synergistic targeting of Sp1, a critical transcription factor for myeloma cell growth and survival, by panobinostat and proteasome inhibitors

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Figure

Figure 1: Sp1 expression in MM cells and MM cell viability by Sp1 inhibition. A. Cell lysates were extracted from MM cell lines as indicated and peripheral blood mononuclear cells (PBMC) isolated from 3 normal donors
Figure 2: Post-translational reduction of Sp1 in MM cells by panobinostat. A. The indicated MM cell lines were cultured in triplicate in the absence or presence of panobinostat at the indicated concentrations
Figure 2 (Continued ): C. RPMI8226 and KMS11 cells were cultured in the absence or presence of panobinostat at the indicated concentrations for 6 and 24 hours, and SP1 mRNA expression was analyzed by RT-PCR
Figure 3: Synergistic reduction of MM cell viability and Sp1 protein levels by panobinostat and proteasome inhibitors
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