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Mec1 Dependent Phosphorylation of the Scc3 Subunit of Cohesin during Mitosis in Budding Yeast

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Figure

Figure 1. Scc3 is phosphorylated on S/T-Q residues following DNA damage. (a) Asynchronous cell cultures of an SCC1-HA2 strain were treated (or not, first lanes) with 0.01% - 0.05% MMS for 2 h at 29˚C
Figure 2. Mass spectrometry analysis of the anti-P-S/T-Q antibody reactive cohesin band
Figure 3. S/T-Q phosphorylation of Scc3 depends on G2/M arrest and can take place independently of DNA damage
Figure 4. S/T-Q phosphorylation of Scc3 takes place during mitosis of an unperturbed cell cycle

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