SECTION 1: PRODUCT AND COMPANY IDENTIFICATION
PRODUCT NAME: Urease Activity Assay Kit (Colorimetric)
PRODUCT CODES: Cat# BN00640
MANUFACTURER: Assay Genie (brand of Reagent Genie Ltd.)
ADDRESS: G1 The Steelworks, Foley Street, Dublin 1
EMERGENCY PHONE: +353 1 8879802
SECTION 2: HAZARDS IDENTIFICATION
Component Description Volume Safety Information
Assay Buffer Proprietary Buffer 15 ml No hazards
Ammonia Reagent 1 Proprietary Buffer (contains phenylphenol) 8 ml See below
Ammonia Reagent 2 Proprietary Solution (contains hypochlorite and NaOH) 4 ml See below
Urea liquid 2 ml No hazards
Urease Lyophilized powder n/a See below
Ammonium Chloride liquid 0.1 ml See below
SECTION 3: COMPOSITION/INFORMATION ON INGREDIENTS
Component CAS Number EC-No. Molecular Weight Chemical Formula
2 Phenylphenol 90-43-7 201-993-5 170.21 C12H10O
Sodium hypochlorite 7681-52-9 231-668-3 74.44 ClNaO
NaOH 1310-73-2 215-185-5 40.00 NaOH
Urease 9002-13-5 232-656-0 90770 n/a
Ammonium Chloride 12125-02-9 235-186-4 53.49 NH4Cl
2-Phenyl phenol:
Emergency Overview
OSHA Hazards:, skin category 2, eye irritation category 2A Target Organs: skin, eyes
GHS Classification: Skin irritation (Category2) Eye irritation (Category 2A)
Respiratory Sensitization (Category 1)
Specific target organ toxicity - single exposure (Category 3), Respiratory system Acute aquatic toxicity (Category 1)
GHS Label elements, including precautionary statements Pictogram:
Signal word: warning
Hazard statement(s): H315: Causes skin irritation H319 Causes serious eye irritation.
H335 May cause respiratory irritation H400 Very toxic to aquatic life.
Precautionary statement(s): P261 Avoid breathing dust/ fume/ gas/ mist/ vapour/ spray.
P264 Wash skin thoroughly after handling.
P271 Use only outdoors or in a well-ventilated area.
P273 Avoid release to the environment.
P280 Wear protective gloves/ eye protection/ face protection.
P285 In case of inadequate ventilation wear respiratory protection.
HMIS Classification Health hazard: 2 Chronic health hazard: * Flammability: 1 Physical hazards: 0 NFPA Rating
Health Hazard: 2 Fire: 1
Reactivity Hazard: 0 Potential Health Effects
Inhalation: harmful if inhaled. Causes respiratory tract irritation.
Skin: May be harmful if absorbed through skin. Causes skin irritation.
Eyes: Causes severe eye irritation.
Ingestion: harmful if swallowed.
Sodium Hypochlorite:
Emergency Overview
OSHA Hazards:, skin category 1B, eye irritation category 2A Target Organs: skin, eyes
GHS Classification: Skin corrosion(Category1B) Serious Eye damage (Category 1) Respiratory Sensitization (Category 1) Acute aquatic toxicity (Category 1) Chronic aquatic toxicity (Category 1) GHS Label elements, including precautionary statements Pictogram:
Signal word: danger
Hazard statement(s): H314 Causes severe skin burns and eye damage H410 Very toxic to aquatic life with long lasting effects.
Precautionary statement(s):
P264 Wash skin thoroughly after handling.
P273 Avoid release to the environment.
P280 Wear protective gloves/ protective clothing/ eye protection/ face protection.
P301 + P330 + P331 IF SWALLOWED: Rinse mouth. Do NOT induce vomiting.
P303 + P361 + P353 IF ON SKIN (or hair): Remove/ Take off immediately all contaminated clothing. Rinse skin with water/ shower.
IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
HMIS Classification Health hazard: 3 Chronic health hazard: * Flammability: 0 Physical hazards: 0 NFPA Rating Health Hazard: 3 Fire: 0
Reactivity Hazard: 0 Potential Health Effects
Inhalation: harmful if inhaled. Causes respiratory tract irritation.
Skin: May be harmful if absorbed through skin. Causes skin irritation.
Eyes: Causes severe eye irritation.
Ingestion: harmful if swallowed.
Sodium Hydroxide:
Emergency Overview
OSHA Hazards: Corrosive to metals, skin category 1A, eye damage category 1 Target Organs: skin, eyes
GHS Classification: Skin corrosion(Category1A) Serious Eye damage (Category 1) Acute aquatic toxicity (Category 1) GHS Label elements, including precautionary statements Pictogram:
Signal word: danger
Hazard statement(s): H290 May be corrosive to metals.
H314 Causes severe skin burns and eye damage H410 Very toxic to aquatic life with long lasting effects.
Precautionary statement(s):
Wash skin thoroughly after handling.
Wear protective gloves/ protective clothing/ eye protection/ face protection.
IF SWALLOWED: Rinse mouth. Do NOT induce vomiting.
IF ON SKIN (or hair): Remove/ Take off immediately all contaminated clothing. Rinse skin with water/ shower.
IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
HMIS Classification Health hazard: 3 Chronic health hazard: * Flammability: 0 Physical hazards: 1 NFPA Rating Health Hazard: 3 Fire: 0
Reactivity Hazard: 0 Potential Health Effects Skin: . Causes skin corrosion.
Eyes: Causes severe eye damage.
Ingestion: harmful if swallowed.
Urease:
Emergency Overview
OSHA Hazards:, skin category 1B, eye irritation category 2A Target Organs: skin, eyes
GHS Classification: Skin corrosion(Category1B) Serious Eye damage (Category 1) Respiratory Sensitization (Category 1) Acute aquatic toxicity (Category 1) Chronic aquatic toxicity (Category 1) GHS Label elements, including precautionary statements Pictogram:
Signal word: danger
Hazard statement(s): H315 Causes skin irritation.
H319 Causes serious eye irritation.
H334 May cause allergy or asthma symptoms or breathing difficulties if inhaled.
Precautionary statement(s): P261 Avoid breathing dust/ fume/ gas/ mist/ vapours/ spray.
P264 Wash skin thoroughly after handling.
P271 Use only outdoors or in a well-ventilated area.
P280 Wear protective gloves/ eye protection/ face protection.
P285 In case of inadequate ventilation wear respiratory protection.
P302 + P352 IF ON SKIN: Wash with plenty of soap and water.
P304 + P340 IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing.
P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P321 Specific treatment (see supplemental first aid instructions on this label).
P332 + P313 If skin irritation occurs: Get medical advice/ attention.
P337 + P313 If eye irritation persists: Get medical advice/ attention.
P342 + P311 If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician.
P362 Take off contaminated clothing and wash before reuse.
P403 + P233 Store in a well-ventilated place. Keep container tightly closed.
P405 Store locked up.
P501 Dispose of contents/ container to an approved waste disposal plant.
HMIS Classification Health hazard: 2 Chronic health hazard: *
Flammability: 0 Physical hazards: 0 NFPA Rating Health Hazard: 2 Fire: 0
Reactivity Hazard: 0 Potential Health Effects
Inhalation: harmful if inhaled. Causes respiratory tract irritation.
Skin: May be harmful if absorbed through skin. Causes skin irritation.
Eyes: Causes severe eye irritation.
Ingestion: harmful if swallowed.
Ammonium Chloride:
Emergency Overview OSHA Hazards: none Target Organs: none
GHS Classification: Acute aquatic toxicity (Category 3) Chronic aquatic toxicity (Category 3) GHS Label elements, including precautionary statements
Pictogram: none
Signal word: none
Hazard statement(s): Harmful to aquatic life with long lasting effects Precautionary statement(s):
P273 Avoid release to the environment.
P501 Dispose of contents/ container to an approved waste disposal plant HMIS Classification
Health hazard: 0 Chronic health hazard: * Flammability: 0 Physical hazards: 0 NFPA Rating Health Hazard: 0 Fire: 0
Reactivity Hazard: 0 Potential Health Effects none
SECTION 4: FIRST AID MEASURES
General advice: Consult a physician. Show this safety data sheet to the doctor in attendance. Move out of dangerous area.
If inhaled: If breathed in, move person into fresh air. If not breathing, give artificial respiration. Consult a physician.
In case of skin contact: Wash off with soap and plenty of water. Consult a physician.
In case of eye contact: Rinse thoroughly with plenty of water for at least 15 minutes and consult a physician.
If swallowed: Never give anything by mouth to an unconscious person. Rinse mouth with water. Consult a physician.
SECTION 5: FIRE-FIGHTING MEASURES
Suitable extinguishing media: Use water spray, alcohol-resistant foam, dry chemical or carbon dioxide.
Special protective equipment for firefighters: Wear self-contained breathing apparatus for firefighting if necessary.
Hazardous combustion products: Hazardous decomposition products not known.
Further information: No data available.
SECTION 6: ACCIDENTAL RELEASE MEASURES
Personal precautions: Use personal protective equipment. Avoid dust formation. Avoid breathing vapors, mist, or gas. Ensure adequate ventilation. Evacuate personnel to safe areas. Avoid breathing dust.
Environmental precautions: Prevent further leakage or spillage if safe to do so. Do not let product enter drains.
Methods for cleaning up: Pick up and arrange disposal without creating dust. Sweep up and shovel. Keep in suitable, closed containers for disposal.
SECTION 7: HANDLING AND STORAGE
Precautions for safe handling
Avoid contact with skin and eyes. Avoid formation of dust and aerosols. Provide appropriate exhaust ventilation at places where dust is formed. Normal measures for preventive fire protection.
Conditions for safe storage
Keep container tightly closed in a dry and well-ventilated place.
Recommended storage temperature: 15-25°C.
SECTION 8: EXPOSURE CONTROLS/PERSONAL PROTECTION
Components with workplace control parameters:
Component s
CAS-No. Value Control
parameters
Basis
sodium
hypochlorite 7681-52-9 STEL 2 mg/m3 USA. Workplace Environmental Exposure
Levels (WEEL) Sodium
hydroxide
1310-73-2 TWA 2.000000 mg/m3 USA. Occupational Exposure Limits
(OSHA) - Table Z-1 Limits for Air Contaminants
C 2.000000 mg/m3 USA. ACGIH Threshold Limit Values (TLV) Remarks Upper Respiratory Tract irritation
Eye irritation Skin irritation
C 2.000000 mg/m3 USA. ACGIH Threshold Limit Values (TLV) Remarks Upper Respiratory Tract irritation
Eye irritation Skin irritation
C 2.000000 mg/m3 USA. NIOSH Recommended
Exposure Limits Ammonium
chloride
12125-02-9 TWA 10.000000 mg/m3 USA. ACGIH Threshold Limit Values
(TLV) Remarks Eye & Upper Respiratory Tract irritation
TWA 10.000000 mg/m3 USA. NIOSH Recommended
Exposure Limits
ST 20.000000 mg/m3 USA. NIOSH Recommended
Exposure Limits
TWA 10.000000 mg/m3 USA. ACGIH Threshold Limit Values (TLV) Upper Respiratory Tract irritation
Eye irritation
STEL 20.000000 mg/m3 USA. ACGIH Threshold Limit Values (TLV) Upper Respiratory Tract irritation
Eye irritation
TWA 10.000000 mg/m3 USA. NIOSH Recommended Exposure Limits
ST 20.000000 mg/m3 USA. NIOSH Recommended Exposure Limits
Personal protective equipment Respiratory protection
Face shield and safety glasses Use equipment for eye protection tested and approved under appropriate government standards such as NIOSH (US) or EN 166(EU)
. Skin protection
Handle with gloves. Gloves must be inspected prior to use. Use proper glove removal technique (without touching glove's outer surface) to avoid skin contact with this product. Dispose of contaminated gloves after use in accordance with applicable laws and good laboratory practices. Wash and dry hands.
Full contact
Material: Nitrile rubber
Minimum layer thickness: 0.11 mm Break through time: 480 min
Material tested:Dermatril® (KCL 740 / Aldrich Z677272, Size M) Splash contact
Material: Nitrile rubber
Minimum layer thickness: 0.11 mm Break through time: 480 min
Material tested:Dermatril® (KCL 740 / Aldrich Z677272, Size M)
data source: KCL GmbH, D-36124 Eichenzell, phone +49 (0)6659 87300, e-mail [email protected], test method:
EN374
If used in solution, or mixed with other substances, and under conditions which differ from EN 374, contact the supplier of the CE approved gloves. This recommendation is advisory only and must be evaluated by an industrial hygienist and safety officer familiar with the specific situation of anticipated use by our customers. It should not be construed as offering an approval for any specific use scenario.
Body protection
Complete suit protecting against chemicals. The type of protective equipment must be selected according to the concentration and amount of the dangerous substance at the specific workplace.
Respiratory protection For nuisance exposures:
Use type P95 (US) or type P1 (EU EN 143) particle respirator.For higher level protection use type OV/AG/P99 (US) or type ABEK-P2 (EU EN 143) respirator cartridges. Use respirators and components tested and approved under appropriate government standards such as NIOSH (US) or CEN (EU).
Hygiene measures
Handle in accordance with good industrial hygiene and safety practice. Wash hands before breaks and at the end of workday.
SECTION 9: PHYSICAL AND CHEMICAL PROPERTIES
Property 2-phenylphenol sodium
hypochlorite
Sodium hydroxide Urease Ammonium Chloride
Appearance: Clear liquid liquid liquid powder clear liquid
pH: No data available no data available no data available no data available 5.4 Melting point: 57-59°C -30 - -20°C no data available no data available no data
available Boiling point: 282°C 111°C no data available no data available no data
available Vapor Pressure 9hPA at 140°C 23.3 hPa at 20°C no data available no data available no data
available Vapor Density No data available No data available no data available no data available no data
available Flash Point (°C): 124°C No data available no data available no data available no data
available Ignition Temperature
(°C): No data available No data available no data available no data available no data available Density: No data available 1.206 g/ml at 25°C no data available no data available .995 g/cm3 at
25°C
SECTION 10: STABILITY AND REACTIVITY 2-Phenylphenol:
Reactivity: No data available
Stability: Stable under recommended storage conditions Possibility of hazardous reactions: No data available Conditions to avoid: No data available
Incompatible materials: Strong oxidizing agents, strong bases
SECTION 11: TOXICOLOGICAL INFORMATION
2-Phenylphenol:
LD50 Oral-rat 2733 mg/kg LD50 IP-rat 50 mg/kg
SECTION 12: ECOLOGICAL INFORMATION
2-Phenylphenol:
Toxicity to fish LC50 - Danio rerio (zebra fish) - 4.5 mg/l - 96 h Toxicity to daphnia and other aquatic invertebrates
LC50 - Daphnia magna (Water flea) - 2.7 mg/l - 48 h Toxicity to algae EC50 - Algae - 0.85 mg/l - 72 h
SECTION 13: DISPOSAL CONSIDERATIONS
Product: Observe all federal, state, and local environmental regulations.
Contaminated packaging: Dispose of as unused product.
SECTION 14: TRANSPORT INFORMATION
2-Phenylphenol:
DOT (US)
Not dangerous goods IMDG
UN number: 3077 Class: 9 Packing group: III EMS-No: F-A, S-F
Proper shipping name: ENVIRONMENTALLY HAZARDOUS SUBSTANCE, SOLID, N.O.S. (Biphenyl -2-ol) Marine pollutant: Marine pollutant
IATA
UN number: 3077 Class: 9 Packing group: III
Proper shipping name: Environmentally hazardous substance, solid, n.o.s. (Biphenyl -2-ol) Further information
EHS-Mark required (ADR 2.2.9.1.10, IMDG code 2.10.3) for single packagings and combination packagings containing inner packagings with Dangerous Goods > 5L for liquids or > 5kg for solids.
Sodium Hypochlorite:
DOT (US) UN number: 1791 Class: 8 Packing group: III
Proper shipping name: Hypochlorite solutions Reportable Quantity (RQ): 667 lbs Poison Inhalation Hazard: No
IMDG UN number: 1791 Class: 8 Packing group: III EMS-No: F-A, S-B Proper shipping name: HYPOCHLORITE SOLUTION
Marine pollutant:yes
IATA UN number: 1791 Class: 8 Packing group: III Proper shipping name: Hypochlorite solution Sodium Hydroxide:
DOT (US)
UN number: 1824 Class: 8 Packing group: II Proper shipping name: Sodium hydroxide solution Poison Inhalation Hazard: No
IMDG
UN number: 1824 Class: 8 Packing group: II EMS-No: F-A, S-B Proper shipping name: SODIUM HYDROXIDE SOLUTION IATA
UN number: 1824 Class: 8 Packing group: II Proper shipping name: Sodium hydroxide solution
SECTION 15: REGULATORY INFORMATION
SARA 302 Components: SARA 302: No chemicals in this material are subject to the reporting requirements of SARA Title III, Section 302.
SARA 313 Components: SARA 313:
Biphenyl-2-ol: CAS-No. 90-43-7 Revision date 1993-04-24 SARA 311/312 Hazards: Acute Health Hazard
Massachusetts Right To Know Components:Biphenyl-2-ol: CAS-No. 90-43-7 Revision date 1993-04-24 sodium hypochlorite: CAS-No. 7681-52-9 Revision date 2007-03-01
Sodium hydroxide: CAS-No. 1310-73-2 Revision Date 2007-03-01 Ammonium chloride CAS-No. 12125-02-9 Revision Date 1994-04-01
Pennsylvania Right To Know Components: Biphenyl-2-ol: CAS-No. 90-43-7 Revision date 1993-04-24 sodium hypochlorite: CAS-No. 7681-52-9 Revision date 2007-03-01
Sodium hydroxide: CAS-No. 1310-73-2 Revision Date 2007-03-01 Urease CAS-No. 9002-13-5 Revision Date
Ammonium chloride CAS-No. 12125-02-9 Revision Date 1994-04-01
New Jersey Right To Know Components: Biphenyl-2-ol: CAS-No. 90-43-7 Revision date 1993-04-24 sodium hypochlorite: CAS-No. 7681-52-9 Revision date 2007-03-01
Sodium hydroxide: CAS-No. 1310-73-2 Revision Date 2007-03-01 Urease CAS-No. 9002-13-5 Revision Date
Ammonium chloride CAS-No. 12125-02-9 Revision Date 1994-04-01
California Prop. 65 Components
WARNING! This product contains a chemical known to the State of California to cause cancer:Biphenyl-2-ol: CAS-No. 90-43-7 Revision date 1993-04-24 SECTION 16: OTHER INFORMATION
DISCLAIMER:
The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide. The information in this document is based on the present state of our knowledge and is applicable to the product with regard to appropriate safety precautions. It does not represent any guarantee of the properties of the product. Assay Genie shall not be held liable for any damage resulting from handling or from contact with the above product. See reverse side of invoice or packing slip for additional terms and conditions of sale.
