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Chromosomes of amphibian oocytes as a model for gene expression: significance of lampbrush loops

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Fig. 2. Visualizationof transcriptionin globularloops(a)RegiOrJof one chromosomeIsola red In a 05MKCI at pH 9 and e),hlbltmgone globularloop(electron microscopy);globular componentsare more or less disorganizedand are due to a dense aggregationof RNP flbnl
Fig. 3. Schematic representationoftranscriptiontor effects on lampbrushphaseinhibi-loops
Fig. 5. SEM of lampbrushchromosomespreads.RNP matrix organization of normal(a), granular(b)and dense (e) loops
Fig. 7. Cold-inducedchanges in lampbrushloops.la)TYPical granular loops (GrUfrom conrrol lampbrushchromosomesat 200C; note the zig-zagarrangementof granules(arrows).(bl After8 daysat goC, typicalhyperdeveloped'cold loops'(CL) are observedin placeof granularloops;someresidualgranules(arrows)are recognizable;notethe remainingspiraledorganizationof theRNP matrix.Bars represenr5}J
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