The in vivo and in vitro effects of bone morphogenetic protein 2 on the development of the chick mandible

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Figure

Fig.2. Percentageof embryoswithaffectedmandiblesfollowingimplantationof agarosebeadsloadedwithdifferentconcentrationsof BMP-2as evidentby whole-mountanalysis.Mandibular malforma-tion is proportionattoBMPconcentration.Allcontrolembryosthat receiveda beadwithoutBMP-2or a beadloadedwithBMP-2at10 ng/plareunaffected.Samplesize is shownaboveeach bar

Fig 2.

Percentageof embryoswithaffectedmandiblesfollowingimplantationof agarosebeadsloadedwithdifferentconcentrationsof BMP 2as evidentby whole mountanalysis Mandibular malforma tion is proportionattoBMPconcentration Allcontrolembryosthat receiveda beadwithoutBMP 2or a beadloadedwithBMP 2at10 ng plareunaffected Samplesize is shownaboveeach bar. View in document p.2
Fig. 1. Effect of local applicationof BMP-2Abbreviations: on mandibulardevelopmentin vivo

Fig 1.

Effect of local applicationof BMP 2Abbreviations on mandibulardevelopmentin vivo. View in document p.2
Fig. 3. Effects

Fig 3.

Effects. View in document p.3
Fig. 4. Histologicalof an embryo

Fig 4.

Histologicalof an embryo. View in document p.4
Fig. 5. Cell death blue sulfate

Fig 5.

Cell death blue sulfate. View in document p.5
Fig.Alcian blue and direct
Fig Alcian blue and direct. View in document p.6
Fig. 7. Expressionof typeplastic.II collagenby mandibularectomesenchymalcellsundergoingchondrogenesisin explantculture.Cryosecrionsof 6-day-old cultureswere stamedwirh a monocfonalantibodyagainstchickenrype /I collagen followedby FITC-conjugatedsecondaryan

Fig 7.

Expressionof typeplastic II collagenby mandibularectomesenchymalcellsundergoingchondrogenesisin explantculture Cryosecrionsof 6 day old cultureswere stamedwirh a monocfonalantibodyagainstchickenrype I collagen followedby FITC conjugatedsecondaryan. View in document p.7
Fig. 8. Expressionof type X collagenby mandibularectomesenchymalcells in explantcultureeitherwith or withoutBMP-2

Fig 8.

Expressionof type X collagenby mandibularectomesenchymalcells in explantcultureeitherwith or withoutBMP 2. View in document p.9
Fig. 9. Histochemicalanalysisof culturesfor mineralizationusing VanKassa's method.Dark granules !'1dicating mineralization of cartilage matnxare eVIdentIn all four groups.(AI Control.culturedon tissuecultureplastIc(BI Control,culturedon typeIV collagen.ICI

Fig 9.

Histochemicalanalysisof culturesfor mineralizationusing VanKassa s method Dark granules 1dicating mineralization of cartilage matnxare eVIdentIn all four groups AI Control culturedon tissuecultureplastIc BI Control culturedon typeIV collagen ICI. View in document p.10

References

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