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Characterization of C-strain “Riems” TAV-epitope escape variants obtained through selective antibody pressure in cell culture

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Table 1 Stability analysis of the E2 TAV escape variants D9, B5/2, Q7, S10, and O11 using partial sequencing of viralproteins E1, E2, and ERNS (numbers indicate nucleotide positions differing from the parental C-strain “Riems”)
Table 3 Results of different C-strain escape variants andsamples from the EPIZONE reference RNA panel in theTAV escape variants specific rRT-PCR system comparedwith the CSF specific rRT-PCR system published byHoffmann et al., 2005 [26]
Figure 2 a/b: Results of virus neutralization tests. Virus neutralization test against CSFV strains “Roesrath” (2a) and “Alfort/187” (2b)
Figure 3 a/b: ELISA results. 3a) E2 specific ELISA (IDEXX) of Q7, S10, O11, or C-strain “Riems” positive control immunized pigs and a mockcontrol of unvaccinated pigs

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