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Postsynaptic densities fragment into subcomplexes upon sonication

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Figure

Fig. 1 Particles that may be fragments of PSDs are produced upon sonication.PSD fractions resuspended in 20 mM HEPES were sonicated with aprobe sonicator as described in Methods; unsonicated control (left panels) and sonicated (right panels) samples were c
Fig. 2 Electron micrographs at low magnification show particle size gradient in pellets from sonicated samples: EM images (1–4) were taken at ~70 nm intervals from the top (1) to near bottom (4) of the pellet from a sonicated sample
Fig. 4 Particles in sonicated samples label for PSD-specific proteins. Pellets from sonicated samples were labeled with antibodies for PSD-95 andSynGAPα2
Fig. 5 Size distribution of particles in control and sonicated samples.An area within 30 μm from the top of each pellet was sampled andthe length (major axis) of PSDs and particles, ~ 40 nm in length andbigger, of similar electron density were measured in

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