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Engineering the cell surface display of cohesins for assembly of cellulosome-inspired enzyme complexes on Lactococcus lactis

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Figure

Figure 1 pAW series ofstrategy of engineered scaffolds and their association with theCipAsecretion of CipAsecretion of CipAof fragments of the gene encoding the cellulosomal scaffold protein CipA, intowith or without an N-terminal nuclease reporter and/or
Figure 2 Growth profiles ofcultures not induced with nisin, panels B, E, H represent cultures induced with 10 ng/mL nisin at inoculation (t = 0 hrs), and panels C, F, Irepresent cultures induced with 10 ng/mL nisin in log phase corresponding to an ODtheir
Figure 3 Cellular localization of NucA-CipAspotting cell fractions on TBD-agar and analyzing for pink color formation
Figure 4 In vivocwa) to ensure saturation of cohesins.(NucA-CBM3a-cwa). Binding assay carried out with UidA and all other constructs resulted in no association with scaffold-expressing strains (datato equivalent amounts of functional cohesin assuming a 1:1
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