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T cell specific adaptor protein (TSAd) promotes interaction of Nck with Lck and SLP-76 in T cells

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Figure

Fig. 1 TSAd-pTyrand F: phenylalanine.SH2 pull-down from 293T cells transiently transfected with Lck and the indicated HA-tagged TSAd cDNA constructs
Fig. 2 The proline rich region (PRR) of TSAd interacts with the Nck SH3 domains.control: GST
Fig. 3 Co-localization of TSAd and Nck in T cells. a Nck co-immunoprecipitation of TSAd expressed in JTAg cells
Fig. 4 TSAd promotes Nck and Lck interaction. a Nck co-immunoprecipitation of TSAd and Lck protein expressed in 293T cells.293T cells transfected with cDNA encoding Nck-GFP, HA-tagged TSAdand Lck were subjected to Nck-IP, and immunoblotted with theindicate
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