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The Golgi apparatus acts as a platform for TBK1 activation after viral RNA sensing

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Figure

Fig. 1 Localization of the active form of TBK1 at the Golgi apparatus after RLR stimulation.either left unstimulated (control) or infected with SeV for 6 h (+ SeV)
Fig. 2 The active form of TBK1 localizes at the Golgi apparatus after TLR3 stimulation.fractionated on OptiPrep density gradients (fractions range from 1 at the top to 4 at the bottom) and analyzed by immunoblotting withantibodies against the indicated pro
Fig. 3 TBK1 forms aggregates at Golgi apparatus following potent RLR activation.proteins were analyzed by immunofluorescence analysis with specific antibodies
Fig. 4 Ubiquitination promotes TBK1 targeting to the Golgi apparatus for activation. a HEK293T cells were transfected with an empty vector (Ev)or with plasmids encoding myc-tagged WT TBK1 (WT), TBK1K38M (K38M), or TBK1K30R/K401R (K30R/K401R)
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