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Absolute Quantification of Enzymes in the Monolignol Biosynthetic Pathway under Transgenic Effect by Mass Spectrometry.

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Figure 1.1 - Monolignol biosynthetic pathway leading to the synthesis of the 3 phenylpropanoid monomers are: p-coumaryl alcohol (H), coniferyl alcohol (G), and sinapyl alcohol (S)
Figure 1.2 - SRM scan mode carried out on triple quadrupole. The precursor mass, product mass are already fixed on the Q1 and Q3 quadrupoles
Figure 1.3 - An example of a 1D SDS-PAGE gel stained with Coomassie blue
Figure 1.4 - Strong cation exchange and strong anion exchange surface interaction scheme
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Materials and Methods Total Proteome Absolute Quantification on the Target Protein of the Transgenic Tree

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