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Efficient homologous recombination mediated genome engineering in zebrafish using TALE nucleases

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Figure

Fig. 1. In vivo(inserted in between the Gs. (C)Ndediencephalon region. (F) Detection ofregion that is similar to that shown in the boxed areas in D-F
Fig. 2. Defining the sizes of homology arms and configurations oftargeting constructs for efficient recombination.239 bp from and the2a-sfGFPrecombination frequency
Fig. 3. Germline transmission rates of sox2-2a-sfGFPlines.were co-injected withwhich was linearized by eitherpercentage of F0 founders that produced sfGFP-positive F1 progeny.(B,C) Comparison of sfGFP expression between strong and weaksox2-2a-sfGFP knock-i
Table 1. Germline transmission of sox2-2a-sfGFP knock-in lines
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