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Detection, Differentiation, and Quantitation of Pathogenic Leishmania Organisms by a Fluorescence Resonance Energy Transfer Based Real Time PCR Assay

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Figure

TABLE 1. Results for Leishmania strains
FIG. 1. Aligned binding regions of oligonucleotides within the 18s rDNA of representative Leishmaniaand designations are given above the alignment
FIG. 2. Determination of the PCR detection limit by probit regres-sion analysis. Negative human blood was spiked with defined amounts
FIG. 3. Melting curve analysis of three reference strains of Leish-mania spp. (solid lines) and cloned rDNAs from the same strains

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