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Cooperative Oligonucleotides Mediating Direct Capture of Hepatitis C Virus RNA from Serum

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Figure

FIG. 1. Schematic representation of the oligonucleotide-assisted capturemethod. The oligonucleotide module (P1) is initially prehybridized to the HCV
FIG. 3. BIAcore analysis of the oligonucleotide-assisted capture of rRNA.An overlay plot of processed sensorgrams (generated by subtraction of the
FIG. 4. Seminested RT-PCR (generating a 273-bp fragment) of in vitro-transcribed HCV rRNA after capture with (A) and without (B) the prehybrid-
FIG. 5. Outer RT-PCR (A and B) and nested PCR (C and D) of a fivefold dilution series of HCV serum

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