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Gluthathione S-transferase-resuscitation-promoting factor B recombinant protein of Mycobacterium tuberculosis induces the production of interferon-γ and interleukin-12 in mice splenocytes

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Figure

Figure 1. Detection and confirmation GST-RpfB expression using SDS-PAGE. (a) SDS-PAGE from crude cells; (b) SDS-PAGE after solubilizing and refolding; Lane 1, GST protein; Lane 2 and 3, supernatants (8 M urea buffer)
Figure 2. Western blot of GST-RpfB after isolation using the affinity of the chromatography column to GST
Figure 3. IL-4, IL-10, IL-12, and IFNγ responses of splenocytes treated by RPMI (negative control), GST, GST-RpfB, and PHA (positive control)

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