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Single-cell TCRseq: paired recovery of entire T-cell alpha and beta chain transcripts in T-cell receptors from single-cell RNAseq

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Academic year: 2020

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Figure

Fig. 1 Schematic overview of scTCRseq pipeline. a Input fastq files are BLAST-aligned to custom reference TCR alpha and beta variable and constantregions genes
Fig. 2 Expression of TCR alpha and beta chain variable gene transcripts relative to the rest of the transcriptome.the mean unadjusted fragments per kilobase per million mapped reads (FPKMs) over all 91 mice T-cell samples, comprising average FPKMs of7990 g
Fig. 3 Count-based analysis of TCR alpha and beta chain variable gene transcripts across the filtered 71 mice T-cell samples.TRAV genes mapping to individual TRAV genes ( a Heatmap of percentageof nucleotide BLAST-mapped TRBV genes mapping to individual TR
Fig. 4 TCR V(D)J sequence usage in Sample ERR488963.underneath the reads denote the TCR beta V gene (TRBV14*01 in a IGV plot of read coverage for entire assembled TCR beta chain sequence

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