P
1997/8 E
UROPEAN
MSPPSA
SERIES
DNA
S
EQUENCING
A
N
A
NALYSIS OF
M
ARKET
S
IZE
& G
ROWTH
M
ARKET
S
HARE
P
URCHASE
P
LANS
&
S
UPPLIER
A
SSESSMENT FOR THE
E
UROPEAN
L
IFE
S
CIENCE
R
ESEARCH
M
ARKET
A
M
ulti-
C
lient
R
eport
by
PhorTech International
San Carlos, California
April 27, 1998
Copyright 1998 by PhorTech International, 238 Crestview Drive, San Carlos CA 94070. All rights reserved. No material contained in this report may be reproduced in whole or in part without the written permission of the publisher. This report is not intended to be, and should not be construed as a recommendation for the purchase or sale of any securities mentioned herein. The information has been derived from statistical and other sources which we deem reliable but their completeness cannot be guaranteed. Opinions expressed herein are based upon our interpretation of available information and are subject to change.
-1-I. B
ACKGROUND
... 9
A.
Survey Objectives... 10
B.
Survey Methodology... 13
II. D
EMOGRAPHIC
S
EGMENTATION
... 17
#0.
Geographic Segmentation of Respondents... 18
#40.
Segmentation by Organizational Type ... 20
#10.
Sequencing Service Utilization... 23
#41.
Providers of Sequencing Services... 25
#42. Respondents Working in Core Facilities ... 28
III. M
ARKET
S
IZE
& G
ROWTH
... 31
#0+.
European Market Size for Life Scientists ... 32
#1.
Current Use of DNA Sequencing, Market Size... 36
#2.
Sharing of Instrumentation: Reported Group Sizes... 43
#3.
Manual Sequencing Unit Installed Base Audit ... 46
#12.
Advanced Sequencing Instruments Installed Base Audit . 54
#A.
Estimate of European Molecular Biology Rgt Market ... 76
IV. M
ARKET
S
HARE
... 79
#3+.
Manual Sequencing Unit & Dollar Market Shares... 80
#12+. Advanced Instrumentation Unit & Dollar Market Shares 89
#27+
Thermal Cycler Unit & Dollar Market Shares ... 100
V. P
URCHASE
P
LANS
... 111
#6.
Forecast Change in Manual Sequencing... 112
#19.
Forecast Change in Automated Sequencing ... 117
#28.
Future Purchase Plans for All Instrumentation... 122
#29.
Instruments & Suppliers Considered for Purchase ... 128
VI. C
URRENT
S
EQUENCING
M
ETHODOLOGY
... 133
#9.
Manual Workload - Templates/Week & Bases Called .... 134
#18.
Automated Workload - Templates/Week & Bases Called 140
#11.
Access to Advanced Instrumentation... 145
#20.
Primary Applications for Automated Sequencing... 147
#21.
Throughput for Non-Sequencing Applications... 151
#22.
Primary Labeling Methods for Automated Sequencing ... 156
#30 .
Sequencing Kit Usage vs. Individual Reagents ... 159
#31.
Suppliers of Sequencing Kits & Reagents... 161
#32.
Current Sequencing Strategies in Use ... 167
#33.
Distribution of Template Types... 170
#34.
Single-Stranded Template Purification Methods... 176
#35.
Plasmid Template Purification Methods ... 179
1997/8 European MSPPSA DNA Sequencing
#37.
Methods for Sequencing Templates ... 185
#38.
Average Consumption by Individual Researchers... 187
#39.
Current Size of Sequencing Project... 196
VII. S
UPPLIER
A
SSESSMENT
... 199
#4.
Reasons for Choosing Last Manual Seq. Purchased... 200
#5.
Customer Satisfaction: Manual Sequencers ... 205
#5+.
Rejected Manual Sequencer Brands & Reasons... 208
#8.
Ranked Supplier Performance - Manual Sequencers ... 213
#13.
Reasons for Choosing Last Automated Seq. Purchased ... 217
#14.
Customer Satisfaction: Automated Sequencers... 222
#14+. Reasons Behind Satisfaction: Automated Sequencers ... 225
#17.
Ranked Supplier Performance: Automated Sequencers ... 229
#23.
Reasons for Choosing Last Robotic Workstation ... 235
#24.
Customer Satisfaction: Robotic Workstations... 237
#26.
Ranked Supplier Performance: Robotic Workstations... 239
VIII. F
UTURE
E
XPECTATIONS
... 245
#7.
Desired Improvements in Manual Sequencers ... 246
#15.
Most Important Features of Automated Sequencers... 249
#16.
Desired Improvements in Automated Sequencers ... 253
#25.
Desired Improvements in Robotic Workstations ... 257
-3-I. B
ACKGROUND... 9
Survey Objectives... 10
Survey Methodology ... 13
Survey Response Rates ... 14
II. D
EMOGRAPHICS
EGMENTATION... 17
Geographic Segmentation for Seven Sources of Names ... 18
Respondent’s Geographic Distribution by European Regions... 19
Distribution by Type of Organization, DNA Sequencing Users ... 20
Distribution by Type of Organization, Automated DNA Sequencing Users ... 21
Distribution by Type of Organization, Manual DNA Sequencing Users ... 21
Utilization of Sequencing Service, All Respondents ... 23
Laboratory Provides Sequencing Service, DNA Sequencing Users ... 25
Laboratory Provides Sequencing Service, All Automated Sequencing Users ... 26
Use vs. Provide Sequencing Service, All Respondents ... 27
Respondents Working in Core Facilities, All Respondents ... 28
Respondents Working in Core Facilities, Automated DNA Sequencing Users... 29
III. M
ARKETS
IZE& G
ROWTH... 31
European Life Scientist Population Estimates by Overlap Analysis ... 33
DNA Sequencing Usage by Source... 37
Eur Population Estimate of DNA Sequencer Users... 38
DNA Sequencing Usage Rate, by Region, Respondents from Unbiased Sources... 38
European DNA Sequencing Users Population Estimate, by Geographic Region ... 39
European Usage of Manual & Automated Sequencers, All Respondents... 40
Usage of Various Instrumentation, All User-Respondents... 41
Reported Group Size, Respondents Performing Sequencing ... 43
Reported Group Size, According to Type of Instrument... 44
Reported Average Group Size for Users, According to Type of Instrument... 44
Mean & Median Number of Manual Sequencers per User, Installed Base ... 45
Total Manual Sequencing Instruments per Respondent/Group ... 47
Average Number of Manual Sequencers per Individual Researcher... 48
Mean & Median Number of Manual Sequencers per Respondent by Region ... 48
Expenditure on Manual Sequencers per Respondent/Group, Installed Base... 49
Mean & Median Expenditure on Manual Sequencers per Respondent by Region. 49
Expenditure on Manual Sequencers per Individual Researcher, Installed Base ... 50
Extrapolation of Installed Base of Manual Sequencers, by Geographic Region ... 51
Unit Placements by Year, Manual DNA Sequencing Unit Installed Base... 51
Dollar Placements by Year, Manual DNA Sequencing Unit Installed Base ... 52
Annual European Manual DNA Sequencer Sales Estimate, by Year... 53
Advanced Sequencing Instrumentation, Unit Share by Category ... 55
Advanced Sequencing Instrumentation, Dollar Share by Category ... 55
1997/8 European MSPPSA DNA Sequencing
Mean & Median Values for Advanced Instruments per Resp, Installed Base ... 56
Median & Mean Number of Automated Sequencers, by Geographic Region... 56
Total Automated Sequencing Instruments, per Individual Researcher... 57
Expenditure on Automated Sequencers, per Respondent’s Group ... 57
Expenditure on Automated Sequencers, per Individual Researcher ... 58
Average Expenditure on Automated Sequencers, by Geographic Region ... 58
Extrapolation of Installed Base of Automated Sequencers, by Geographic Region . 59 Units Placements by Year, Automated Sequencers Installed Base ... 59
Dollar Placements by Year, Automated Sequencers Installed Base ... 60
Annual European Automated DNA Sequencer Sales Estimate, by Year... 60
Calculated CAGR for Automated Sequencers, 1994 to present... 61
Number of Other Advanced Instruments per Respondent’s Group... 61
Number of Other Advanced Instruments per Individual Researcher ... 62
Average Quantity of Robotic Workstation & Software Used for Sequencing... 62
Expenditure on Robotic Workstations, per Respondent’s Group ... 63
Expenditure on Robotic Workstations, per Individual Researcher... 63
Expenditure on Sequencing Analysis Software, per Respondent’s Group... 64
Expenditure on Sequencing Analysis Software, per Individual Researcher ... 64
Average Expenditure on Robotic Wkstns & Software, by Region... 65
Extrapolation of Installed Base of Robotic Workstations, by Geographic Region .. 65
Extrapolation of Installed Base of Analysis Software, by Geographic Region ... 65
Units Placements by Year, Robotic Workstation Installed Base... 66
Dollar Placements by Year, Robotic Workstation Installed Base ... 66
Annual European Robotic Workstation Sales Estimate, by Year... 67
Units Placements by Year, Sequence Analysis Software Installed Base ... 67
Dollar Placements by Year, Sequence Analysis Software Installed Base... 68
Annual European Sequencing Analysis Software Sales Estimate, by Year... 68
Average Number of Thermal Cyclers Reported per Respondent’s Group... 70
Average Number of Thermal Cyclers Reported per Individual Researcher ... 70
Median & Mean Number of Thermal Cyclers, by Geographic Region ... 71
Expenditure on Thermal Cyclers, per Respondent/Group ... 71
Expenditure on Thermal Cyclers, per Individual Researcher ... 72
Average Expenditure on Thermal Cyclers, by Geographic Region... 72
Extrapolation of Installed Base of Thermal Cyclers, by Geographic Region... 73
Units Placements by Year, Thermal Cycler Installed Base ... 73
Dollar Placements by Year, Thermal Cycler Installed Base ... 74
Annual European Thermal Cycler (for Sequencing) Sales Estimate, by Year ... 74
European Sales Estimate for All Sequencing Instrumentation, by Year ... 76
Dollar Market Worth by Geographic Region, Automated Sequencing Units ... 77
IV. M
ARKETS
HARE... 79
Manufacturers’ Unit Market Shares for Manual DNA Sequencers... 80
Manufacturers’ Dollar Market Shares for Manual DNA Sequencers ... 81
Average Manual Sequencer Prices for Major Suppliers... 82
-5-Manufacturers’ $ Market Shares for Manual Sequencers, English-speaking Rgn ... 85
Manufacturers’ $ Market Shares for Manual Sequencers, German-speaking Rgn.. 86
Manufacturers’ $ Market Shares for Manual Sequencers, Scandinavian Rgn... 86
Manufacturers’ $ Market Shares for Manual Sequencers, France & Southern Eur 87
Manufacturers’ $ Market Shares for Manual Sequencers, Benelux ... 88
Manufacturers’ Unit Market Shares, Automated Sequencer Installed Base... 89
Manufacturers’ Dollar Market Shares, Automated Sequencer Installed Base ... 90
Most Frequently Mentioned Automated Sequencer Models ... 91
Manufacturers’ Dollar Market Shares, Automated Sequencers Sold Since 1994.... 91
Manufacturers’ $ Market Shares for Auto Sequencers, English-speaking Rgn ... 92
Manufacturers’ $ Market Shares for Auto Sequencers, German-speaking Rgn ... 93
Manufacturers’ $ Market Shares for Auto Sequencers, Scandinavian Rgn ... 93
Manufacturers’ $ Market Shares for Auto Sequencers, France & Southern Eur... 94
Manufacturers’ $ Market Shares for Auto Sequencers, Benelux ... 94
Manufacturers’ Unit Market Shares, Robotic Workstation Installed Base ... 95
Manufacturers’ Dollar Market Shares, Robotic Workstation Installed Base ... 96
Average Robotic Workstation Prices for Major Suppliers... 96
Manufacturers’ Unit Market Shares, Sequence Analysis Software Installed Base ... 97
Manufacturers’ Dollar Market Share, Sequence Analysis Software Installed Base .. 97
Average Sequence Analysis Software Prices for Major Suppliers ... 98
Most Frequently Mentioned Sequencing Analysis Software Models... 98
Manufacturers’ Unit Market Shares, Thermal Cycler Installed Base ... 100
Manufacturers’ Dollar Market Share, Thermal Cycler Installed Base... 101
Average Prices for Major Suppliers of Thermal Cyclers ... 102
Thermal Cycler Sample Capacity, Share of Installed Base... 103
Average Price Paid for Various Thermal Cycler Sample Capacities ... 103
Thermal Cycler Sample Capacity, Dollar Market Share ... 104
Most Frequently Mentioned Thermal Cycler Models... 104
Manufacturers’ Dollar Market Share, Thermal Cyclers Sold Since 1994 ... 105
Manufacturers’ $ Market Shares for Thermal Cyclers, English-speaking Rgn ... 106
Manufacturers’ $ Market Shares for Thermal Cyclers, German-speaking Rgn ... 106
Manufacturers’ $ Market Shares for Manual Sequencers, Southern Europe ... 107
Manufacturers’ $ Market Shares for Manual Sequencers, France ... 107
Manufacturers’ $ Market Shares for Thermal Cyclers, Scandinavian Rgn ... 108
Manufacturers’ $ Market Shares for Manual Sequencers, Benelux ... 109
V. P
URCHASEP
LANS... 111
Forecast Change in Manual Sequencing, All Manual Sequencing Users... 113
Aver. Change in Manual Sequencing Over Next 12 Months by Current Users... 113
Verbatim Comments Reasons Behind Anticipated Change in Manual Sequencing 114 Verbatim Comments From Previous Manual Sequencing Users ... 116
Forecast Change in Automated Sequencing, Automated Sequencing Users... 118
Average Change in Auto Sequencing Over Next 12 Months by Current Users ... 118 Verbatim Comments Regarding Forecast Change in Automated Sequencing Use. 119
1997/8 European MSPPSA DNA Sequencing
Purchase Plans for Auto Sequencers, Response Profiles of Users & Non-Users ... 122
1997/98 European Sales Projections for Automated Sequencers ... 123
Purchase Plans for Robotic Workstations, Response Profile of Current Users ... 124
1997/98 European Sales Projections for Robotic Workstations... 124
Purchase Plans for Analysis Software, Response Profile of Current Users ... 125
1997/98 European Sales Projections for Analysis Software... 125
Purchase Plans for Thermal Cyclers, Response Profile of Current Users ... 126
1997/98 Eur Sales Projections for Thermal Cyclers Used for Cycle Sequencing.... 127
Share of Mentions, Planned Purchases of Automated DNA Sequencers... 128
Share of Mentions, Planned Purchases of Robotic Workstations... 129
Share of Mentions, Planned Purchases of Sequencing Analysis Software ... 130
Share of Mentions, Planned Purchases of Thermal Cyclers ... 130
VI. C
URRENTS
EQUENCINGM
ETHODOLOGY... 133
Average Templates Sequenced per Week, Cross Section of Manual Seq. Users ... 135
Mean, Median & Mode: Templates Sequenced/Week by Manual Sequencing... 135
Mean, Median & Mode: Average Gels Run per Week by Manual Sequencing... 135
Average Gels Run per Week, Cross Section of Manual Seq. Users ... 136
Mean, Median & Mode: Average Bases Called/Template by Manual Sequencing. 136 Average Bases Called per Template, Cross Section of Manual Sequencing Users .. 137
Average Bases Sequenced per Week, Cross Section of Manual Sequencing Users . 137 Mean, Median & Mode: Average Bases Sequenced/Week by Manual Sequencing 137 Average Reagent Cost per Template, Cross Section of Manual Seq. Users ... 138
Mean, Median & Mode: Reagent Cost/Template Using Manual Sequencing ... 138
Mean, Median & Mode: Average Reagent Cost/Week Using Manual Sequencing 138 Average Reagent Cost per Week, Cross Section of Manual Sequencing Users ... 139
Summary of Mean & Median Values for Manual Sequencing Users ... 139
Average Templates Sequenced per Week, Cross Section of Automated Seq Users 140 Mean, Median & Mode: Average Templates Sequenced/Week by Auto Seq ... 140
Mean, Median & Mode: Bases Called per Template by Automated Sequencing ... 141
Average Bases per Template, Cross Section of Automated Seq. Users ... 141
Average Bases Sequenced per Week Called, Cross Sectn of Automated Seq. Users 142 Mean & Median: Average Reagent Cost/Template by Automated Sequencing ... 142
Average Reagent Cost per Template, Cross Section of Automated Seq. Users ... 143
Average Reagent Cost per Week, Cross Section of Automated Sequencing Users . 143 Summary of Mean & Median Values for Automated Sequencing Users... 144
Access to Sequencing Instrumentation, Current Users ... 146
Verbatim Description of Applications for Automated Sequencing... 147
Percentage Throughput for Non-Sequencing Applications ... 151
Non-Sequencing Applications, Percentage Throughput on Auto Sequencers ... 152
Verbatim Description of Non-Sequencing Applications & Level of Throughput .. 153
Non-Sequencing Applications, Share of Mentions... 154
Primary Labeling Methods Utilized by Automated Sequencing Users ... 156
Sequencing Labeling Methods, Dye Primer vs Dideoxy Dye Terminator... 157
Verbatim Description of Other Labeling Methods for Automated Sequencing... 157
-7-Preferred Brands of Sequencing Enzymes, Share of Mentions... 162
Preferred Brands of Nucleotides, Share of Mentions... 163
Preferred Brands of Sequencing Buffers, Share of Mentions ... 164
Preferred Brands of Gel Matrix Reagents, Share of Mentions ... 164
Preferred Brands of Other Reagents, Share of Mentions ... 165
Current Sequencing Strategies in Use... 167
Verbatim Description Regarding Other Sequencing Strategies Used... 168
Current Sequencing Strategies in Use, Manual vs. Automated Sequencing Users.. 169
Template Preparation Techniques, Share of Mentions ... 170
Percentage of Total Templates, PCR Generated... 171
Percentage of Total Templates, Plasmid Vector... 172
Percentage of Total Templates, M13/Phagemid Vector... 172
Percentage of Total Templates, Lambda/Cosmid Vector ... 173
Percentage of Total Templates, Other Template Types ... 174
Write-In Responses for Sequencing Templates ... 174
Average Number of Templates, All Users vs. Automated Sequencing Users ... 175
Verbatim Description Regarding Single Stranded Template Purification Method. 176 Single Stranded Template Purification Methods, Reported Brand Names ... 178
Plasmid Template Purification, All Sequencing Users... 179
Verbatim Description of Other Methods for Plasmid Template Purification ... 180
Plasmid Template Purification, Manual vs. Automated Sequencing Users ... 181
PCR-Generated Template Purification, All Sequencing Users ... 182
Verbatim Descriptn of Other Methods for PCR-Generated Template Purificat’n. 183 PCR-Generated Template Purification, Manual vs. Automated Sequencing Users 184 Current Methods for Sequencing Templates, All Users ... 185
Current Methods for Sequencing Templates, Manual vs. Automated Sequencing 186 Sequencing Enzyme Use, Share of Mentions ... 187
Templates Sequenced per Week, Sequenase ... 188
Templates Sequenced per Week, AmpliTaq FS ... 189
Templates Sequenced per Week, Thermosequenase... 189
Templates Sequenced per Week, Taq Polymerase... 190
Templates Sequencing per Week, AmpliTaq CS ... 190
Templates Sequenced per Week, Sequitherm ... 191
Templates Sequenced per Week, Reverse Transcriptase... 192
Templates Sequenced per Week, Bst Polymerase... 192
Templates Sequenced per Week, Other Enzymes ... 193
Median Number of Templates Sequenced Weekly, Various Sequencing Enzymes 194 Share of All Templates Used Weekly for Each Sequencing Enzyme ... 194
Sequencing Enzyme Use, Manual vs. Automated Sequencing Users ... 195
Monthly Sequencing Output, All Sequencing Users... 196
Monthly Sequencing Output, Automated Sequencing Users ... 197
VII. S
UPPLIERA
SSESSMENT... 199
1997/8 European MSPPSA DNA Sequencing
Verbatim Comments for Last Purchased Manual Sequencer Sorted by Brand ... 201
Satisfaction with Last Purchased Manual Sequencer ... 205
Customer Satisfaction By Last Purchased Brand of Manual Sequencer... 206
Satisfaction Rates & Confidence Levels for Manual Sequencer Manufacturers ... 207
Reasons for Dissatisfaction with Last Manual Seq. Purchase Sorted by Brand ... 208
Reasons for Satisfaction with Last Manual Sequencer Purchase Sorted by Brand... 209
Ranked Manufacturers’ Performance for Manual Seq.: Best Value for Money ... 213
Ranked Manufacturers’ Performance for Manual Seq.: Easiest to Use... 214
Ranked Manufacturers’ Performance for Manual Seq.: Most Reliable Quality ... 215
Ranked Manufacturers’ Performance for Manual Seq.: Best Tech Support ... 215
Last Automated Sequencer Purchased, All Users... 217
Verbatim Comments for Last Purchased Automated Sequencer Sorted by Brand.. 218
Satisfaction with Last Purchased Automated Sequencer, Share of Mentions ... 222
Satisfaction Ratings By Last Purchased Brand of Automated Sequencer ... 223
Satisfaction Rates & Confidence Levels for Automated Sequencer Manufacturers. 224 Reasons for Dissatisfaction with Last Automated Seq. Purchase Sorted by Brand .. 225
Reasons for Satisfaction with Last Automated Seq. Purchase Sorted by Brand... 226
Ranked Manufacturers’ Performance for Automated: Best Field Service ... 229
Ranked Manufacturers’ Performance for Automated: Easiest to Use ... 230
Ranked Manufacturers’ Performance for Automated: Greatest Innovator ... 231
Ranked Manufacturers’ Performance for Automated: Highest Throughput ... 231
Ranked Manufacturers’ Performance for Automated: Lowest Running Costs... 232
Ranked Manufacturers’ Performance for Automated: Most Reliable Quality ... 232
Ranked Manufacturers’ Performance for Automated: Most Committed... 233
Last Robotic Workstation Purchased, All Users ... 235
Reason for Selecting Brand of Robotic Workstation Sorted by Brand ... 236
Satisfaction with Last Purchased Robotic Workstation... 237
Verbatim Comments Regarding Dissatisfaction with Robotic Wkstn, by Brand ... 238
Verbatim Comments Regarding Satisfaction with Robotic Wkstn, by Brand ... 238
Ranked Manufacturers’ Performance for Robotics: Application Flexibility ... 239
Ranked Manufacturers’ Performance for Robotics: Best Field Service ... 240
Ranked Manufacturers’ Performance for Robotics: Easiest to Use... 241
Ranked Manufacturers’ Performance for Robotics: Level of Automation ... 241
Ranked Manufacturers’ Performance for Robotics: Most Reliable Quality ... 242
VIII. F
UTUREE
XPECTATIONS... 245
Suggested Improvements in Manual DNA Sequencers ... 246
Most Frequently Mentioned Areas for Improvement, Manual Sequencers ... 248
Most Important Features of Automated Sequencers... 249
Most Frequently Mentioned Features, Automated Sequencers ... 252
Suggested Improvements in Automated DNA Sequencers ... 253
Suggested Improvements in Automation Capabilities ... 257