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Characterization of ovine SRY transcript and developmental expression of genes involved in sexual differentiation

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Academic year: 2020

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Figure

Fig. 1. Resultsof 5'RACE experiments.(A) Diagrammaticrepresentationof SRY transcription start sites.The synthesisof single-strandedcDNAwas primed using random hexanucleoridesand 5 #)9of total RNAextractedfrom 30 dpc male fetal gonads and from 35 dpp testes
Fig. 4. SRY expressioncycles)day-oldreversein postnataltestes.RT-PCRamplification(30from differenttesticularsamplesusingsheepSRY primers(M2-M9J
Fig. 6. Timecourseof P450 aromataseICYP191. SF-1. AMH. WT-'and ZFX expressionduringfemalegonadaldevelopmentin sheep.Ethidiumbromide-stainedagarose gels showing RT-PCR productsobtainedfromfetal and adult female gonads with differenr primer pairsafter 30 cyclesof ampllficarion.All sampleswere posirive for ZFX,except when RTwas omirred(-).
Fig. 8. AMH expressionin postnataltestes.Samples were taken atdifferentstagesof developmentfrom birth untIl adult

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