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Modulation of the epidermal growth factor receptor of mouse embryonic palatal mesenchyme cells in vitro by growth factors

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Figure

Fig. 2. The effect of 1251_EGFconcentrationon bindingat 20ce.to MEPM cellsCells were cultured at a confluent(5 x 7oJ cells/wefl,O) or sub-confluent(2.2 x 10J cells/well,.)density.Cells were incubatedwith theIndicatedconcentrationof 125/_EGF for 90 min at 2
Fig. 4. Effect of TGF-B, fA), IGF-II IB) and bFGF IC) pre-treatmentonspecificperiod specificseparatebindingof '251_EGFby MEPM cells
TABLE 3
Fig. 5. Effect of bFGF, IGF-II and TGF-Bculturedpresence on 1251_EGFbindingcapacityofMEPM cells.Cells were pre-treatedfor 4 h (A) or 24 h (B) in rheor absence(J) ofbFGF(1ng/ml, .!,IGF-I/(100ng/ml,I!or TGF-61 (1 ng/ml.8;Afterpre-treatmentthespecificbindingof increasingconcentrationsof 125f_EGF (2.5-20 ng/ml) was determinedas describedinMaterialsand Methods, and expressed as a Scatchard plot.

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