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The energy landscape of −1 ribosomal frameshifting

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Academic year: 2020

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Figure

Fig. 1. mRNA structure in −1 frameshifting induces translocation pause. (slippery sequence and 3′ mRNA structure (nts: nucleotides)
Fig. 2. Characterizing the temperature dependence of translation through the mRNA structure
Fig. 3. EF-G hydrolyzes multiple GTPs to unfold mRNA structure during translocation. (A) Translocation is monitored via tRNA–EF-G FRET efficiency changes from pretranslocation (Cy3 within the A/P site) to posttranslocation (Cy3 within the P site)
Fig. 4. mRNA structure enhances frameshifting by lengthening the rotated state. (A) mRNA constructs containing frameshifting cassette and different mRNA structures (SL, stem-loop; PK, pseudoknot; and Unstruc., unstructured) used in the programmed −1 frames
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