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Expression and Purification of Human Interferon Gamma Using a Plant Viral Vector

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Academic year: 2020

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Figure

Figure 1. Construction of ZYMV-INF-γ and examining of its infectivity. A. Schematic representation of ZYMV-INF-γ full genome
Figure 2. Detection and examining the stability of ZYMV-INF-γ vector in squash. A. The RT-PCR with INF-γ specific primers in the ZYMV genome should yield 448 bp products from ZYMV-INF-γ-infected squash
Figure 3. Examining of stability of ZYMV- INF-γ after six passages. A. RT-PCR amplification profile from squash plants infected with ZYMV- INF-γ vectors after 1-6 serial passages corresponding to lane 1-6; lane 7: negative control (ZYMV-WT)
Figure 4. Purification of INF-γ proteins expressed by ZYMV-INF-γ in infected zucchini squash plants by affinity chromatography

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