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Analysis of normal and malignant human B cell growth using gene transfer

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Figure

Figure 1.1: Schem e of haemopoiesis.
Figure 1.2: Diagram of B cell development.
Figure 1.3: Schematic diagram of Ig heavy chain rearrangement.
Figure 1.4: Immunoglobulin isotype switching.
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B Cell Malignancies: Materials and Methods: 10^ cells per 200 pi RPMI/10% FCS were maintained in 96 well flat bottom tissue culture plates (Nunc) in the presence or absence of stimuli Six hours before Discussion: Construction of Eukaryotic Expression Vectors for Gene and 3 of the human c-myc gene were excised from the expression vector pMLV-myc (Lee et al 1985) using the enzymes Xba I and Eco RI The resulting 2.4 kb CLL cells were analyzed for phenotypic changes 72 hours after transfection pCMV-LTR-c-myc transfected and cells electroporated with pCMV-LTR-poly were stained CLL cells were electroporated with a c-myc containing plasmid and control sequences (pCMV LTR-poly) After 72 hours culture in RPMI/10% PCS cells were stained with CD11a antibodies and CLL cells were analyzed for expression of a cell cycle related antigen recognized by the monoclonal antibody Ki-67 This antigen is only expressed by proliferating cells Conclusions

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