Training Courses 2014 HPLC GC SPE

HPLC

How to Run HPLC Methods Price: £ 249 + VAT Code: SS0-5943 Page: 4

How to Develop HPLC Methods Price: £ 249 + VAT Code: SS0-5947 Page: 5

How to Develop HPLC Methods for Challenging Separations

Price: £ 249 + VAT Code: SS0-5946 Page: 6

How to Troubleshoot HPLC Price: £ 249 + VAT Code: SS0-7449 Page: 8

Courses & Presenters

Meet the presenter:

Oona McPolin

Oona has considerable experience and is fully qualified in the areas of both pharmaceutical analysis and training practice. She has worked as an analytical chemist in the pharmaceutical industry for over 10 years on a range of drug development projects and has been responsible for many pharmaceutical analysis training programmes during this time. Her area of expertise is in the technique of HPLC with particular interest in strategies for method development. Oona is the author of two books, “An Introduction to HPLC for Pharmaceutical Analysis” and “Validation of Analytical Methods for Pharmaceutical Analysis”. Oona has obtained the industry standard qualification for training, the Certificate in Training Practice, awarded by the Chartered Institute of Personnel and Development (CIPD).

Qualifications:

• 1993 BSc Hons Chemistry, Queen’s University of Belfast

• 1994 MSc Applied Environmental Sciences, Queen’s University of Belfast

• 2008 Certificate in Training Practice, Chartered Institute of Personnel and Development (CIPD). Received a High Achiever’s Award from the CIPD NI branch

Affiliations:

• Member of the Royal Society of Chemistry (MRSC) • Chartered Chemist (CChem)

5 10 15 20 min 1 23 45 6 7 8

sPe

The SPE Method Developers Seminar Code: SS0-9233 Page: 10

GC

The Gas Chromatography Seminar Code: SS0-7292 Page: 9

5 10 15 20 min 1 23 45 67 8

Training at your site?

Ask your technical consultant about training for you and your colleagues, at your site.

Save time / Save costs with first class training for your whole team.

every course includes:

• First class training

• Training materials booklet

• Lunch and refreshments

• Post-training online assessment, with certificate of completion

Meet the presenter:

Melanie emmanuel

Melanie Emmanuel graduated from University College London with a BSc in Pharmacology. She joined Phenomenex in 2004 as an Account Manager focusing on our Gas Chromatography product line.

After three years of working across all industries Melanie was promoted in 2008 to Key Accounts Manager and Group Leader. As a Key Account Manager she has become an industry expert in both the Toxicology and Environmental markets. Her role allows her to develop customer methods in our lab, give technical presentations both at conferences and at customer facilities, and visit hundreds of labs across the USA. She also assists with new product development to ensure that our gas chromatography line continues to strengthen and grow. It is because of her knowledge, expertise and in credible work ethic she was selected to lead training for our Singaporean distributor. Melanie also ran this successful series of GC seminars across the Nordic region and the UK in 2013.

Meet the presenter:

Dr. Raymond Wong

Dr. Raymond Wong’s background is in Synthetic organic chemistry. He completed his Ph.D.in synthesis of pyrrolidine based oligonucleotides, using solid phase peptide protocols. Additionally, his Post doctoral research is in dynamic combinatorial libraries using poly ethylene glycol resin beads. Following his role as a developmental chemist purifying active pharmaceutical compounds up to multi-kilo scale,

Raymond joined Phenomenex.

Solid Phase Extraction

During his 4 years with Phenomenex, Raymond has become the SPE Specialist for customers in the UK and Ireland. He has considerable experience and is fully qualified in the areas of phar-maceutical analysis, specializing in sample preparation techniques such as Solid Phase Extraction (SPE). His knowledge reaches from drugs of abuse in toxicology, to polyaromatic hydrocarbons sample preparation in the environmental sector.

A key responsibility as SPE Specialist at Phenomenex, involves Raymond delivering dynamic, customized training courses. This has included both theoretical and practical learning programs at customer facilities.

Course summary:

Learn how to set up and run HPLC analysis with full understanding of all the method parameters such as the column, the mobile phase, the instrumentation, and sample preparation, and how to interpret and quantify the results of the analysis. This course is ideal for those who are new to HPLC.

Course outline:

HPLC method parameters

• Instrumentation

• Preparation of test solutions • Directions for analysis

1]

How to Run

HPLC

Methods

Course no. ss0-5943

Practical skills acquired:

This course will enable you to implement HPLC analytical methods by transferring the parameters from the method to your HPLC system. In addition you will be able to:

1. Understand what is meant by all the parameters in an HPLC analytical method. 2. Follow an HPLC analytical method to set up an HPLC system for analysis. 3. Run an HPLC analytical method and acquire chromatographic results. 4. Interpret chromatograms obtained from HPLC analysis.

5. Calculate analytical results for HPLC analysis.

Dates: 10.03.2014

London

13.10.2014

Warrington

setting up HPLC systems for analysis

Interpreting results from HPLC analysis

• Integration • System suitability • Quantification techniques

HPLC

1]

How to Run

HPLC

Methods

Course no. ss0-5943

Practical skills acquired:

This course will enable you to take a strategic approach to developing HPLC methods with an understanding of the factors which can be adjusted to manipulate the retention time of analytes. In addition you will be able to:

1. Define the objectives for the development of an HPLC analytical method. 2. Effectively assess all the available relevant information relating to the desired method, e.g. pKa of the analyte.

3. Select and prepare a suitable sample or samples to be used for the method development.

4. Select suitable scouting conditions to find a suitable column and mobile phase system.

5. Optimise the chromatographic conditions to result in the best possible separation.

Dates: 11.03.2014 London 14.10.2014 Warrington

Course summary:

Learn how to select appropriate method conditions and perform suitable investigative experiments to obtain a set of method parameters which enables the desired separation for mixtures of analytes.

This course is ideal for those who have experience running HPLC methods and now want to learn how to develop new methods.

Course outline:

Developing an HPLC method using a 5-step strategy:

3. Selecting suitable samples

4. Performing scouting experiments to select suitable initial conditions

5. Optimising the method to define method parameters which achieve the desired separation This course focuses on reversed phase mode separations.

2]

How to develop

HPLC

Methods

Course summary:

Learn how to implement strategies to achieve satisfactory separation for ‘complex’ samples and use computer modelling to develop robust and fit for purpose HPLC methods. This one day course is ideal for those who have experience of developing HPLC methods but want to increase their knowledge to deal with more challenging separations. Although designed as a follow-up to the course “How to Develop HPLC Methods”, it is not a prerequisite. This course is suitable for learners who feel confident that they are familiar with all the content detailed for the course “How to Develop HPLC Methods”.

Course outline:

Implementing a 5-step strategy for method development of complex samples

Review of a 5-step strategy for HPLC method development with particular attention to reasons why some separations are ‘challenging’:

1: Setting suitable objectives for method development 2: Assessing all available information

3: Selecting suitable samples

4: Performing scouting experiments to select suitable initial conditions

5: Optimising the method to define method parameters which achieve the desired separation

separation Theory

Method development objectives: Strategies for maximising resolution in HPLC using retention factor, k’, selectivity, α, and efficiency, N.

3]

How to develop

HPLC

Methods for

HPLC

Practical skills acquired:

This course will enable you to find solutions for difficult HPLC separations. In addition you will be able to:

1. Understand why some separations can be challenging. 2. Identify potential problem separations.

Apply strategies to achieve satisfactory separations for “complex” samples with respect to: 1. Sample preparation

2. Detection methods

3. Retaining very polar analytes 4. Optimising gradient methods 5. Selecting columns

6. Setting up scouting/screening experiments

Dates: 14.04.2014 Edinburgh 08.05.2014 Warrington 15.09.2014 London

Case study Using Computer Modelling

A case study is used to demonstrate how a computer model may be used to find the best conditions for the separation.

strategy 1: sample Preparation

A general approach to sample preparation is provided with considerations regarding the most suitable technique.

strategy 2: Detection Techniques

How to choose a suitable detector when analytes are not UV active: the different types of detectors used for HPLC, deriva-tisation to improve detectability.

strategy 3: Retaining Very Polar Analytes

Techniques which enable analysis of very polar molecules by reversed phase HPLC: High pH, aqua columns, mixed mode columns, HILIC, Ion-pairing chromatography.

strategy 4: Optimising Gradient Methods

Understanding gradient analysis; using multi-segment gradients for complex mixtures of analytes.

strategy 5: selecting Columns

Selecting columns which give different selectivity – tools for column comparison.

strategy 6: scouting/screening experiments

Course summary:

Learn how to find solutions for problems encountered when running HPLC analysis by diagnosing symptoms and implementing appropriate preventative measures.

This course is ideal for those who have experience using HPLC and now want to develop their skills further.

Course outline:

• Overview of the HPLC and how it works:

Mobile phase, pumps, injectors, columns, detectors and connections • Common problems and preventative measures

• Problem solving strategy: Assessing the symptoms Making diagnosis

Finding the appropriate solution

4]

How to troubleshoot

HPLC

Course no. ss0-7449

Practical skills acquired:

This course will enable you to go back to your lab with a full understanding of why problems may arise with your HPLC system and give you the skills and knowledge to both prevent and resolve those problems. In addition you will be able to: 1. Understand how HPLC works and the role of each component in an HPLC system.

2. Understand how problems can arise in the individual components of an HPLC system. 3. Implement measures which prevent problems occurring.

4. Use a systematic problem-solving approach to HPLC troubleshooting. 5. Diagnose and resolve problems associated with HPLC.

Dates: 09.05.2014 Warrington 16.06.2014 Edinburgh 16.09.2014 London

gC

This course is ideal for:

• Scientists new to Gas Chromatography

• Chromatographers with experience of running GC methods, but looking to develop new methods

• Gas Chromatographers wishing to refresh their knowledge of GC and to learn new troubleshooting tips

Dates: 23.06.2014 Warrington 24.06.2014 London 25.06.2014 Newcastle

Course summary

Join us for this 1 day training course, which provides a comprehensive working knowledge of GC methods, systems and consumables.

Course Outline

Areas of GC covered:

• The Basics of Gas Chromatography

• Inlets, Detectors, Columns and Accessories

5]

Gas Chromatography

Seminar

Course summary:

An in depth course that will demonstrate how to instantly improve GC and HPLC analysis through the use of Solid Phase Extraction (SPE).

6]

sPe

Method developers Seminar

This course is ideal for:

Any analyst who wishes to improve their GC and HPLC analysis.

Previous knowledge:

An understanding of the principles of chromatrography would be advantageous. Previous knowledge of SPE is not required.

Dates: 13.05.2014 Edinburgh 14.05.2014 Belfast 17.06.2014 London 18.06.2014 Warrington

sPe separation Mechanisms

• Reversed-phase SPE • Normal phase SPE • Cation-Exchange SPE • Anion-Exchange SPE • Mixed-Mode SPE

Course Outline:

sPe extraction Protocol

• SPE Sample Pre-treatment • SPE Column Conditioning • SPE Column Equilibration • Sample Loading

• Column Washing • Analyte Elution

Each course includes full course instructions, simple SPE protocol and a starter pack of SPE tubes, to get started once back in the lab.

Molecular Properties

• Hydrophobic or Non-Polar Groups • Polar Groups

• Ionic Groups • Chelating Groups

sPe Method Development

• Analyte Assessment • Mechanism Selection • Sorbent Screening • Procedure Optimization

6]

sPe

Method developers Seminar

Registration Form

Course Registration

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Terms and ConditionsSubject to Phenomenex Standard Terms & Conditions, which may be viewed at www. phenomenex.com/TermsAndConditions.