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Subtyping Clinical Specimens of Influenza A Virus by Use of a Simple Method To Amplify RNA Targets

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Figure

FIG 1 Overview of SMART. (1) Streptavidin-coated beads conjugated with biotinylated capture oligonucleotides and SMART probes are added to the solutionof vRNA isolated from clinical samples
TABLE 1 Oligonucleotides used in the SMART and RT-PCRa
FIG 2 Standard curves relating the time to positivity to known vRNA concentrations (log scale)
FIG 3 Real-time SMART amplification with clinical samples. Normalized real-time SMART amplification curves of clinical samples 8, 98, and 106 whenanalyzed with H3, H1, and H1p probes
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