A split -galactosidase genome-wide RNAi screen for
A genome-wide RNAi screen reveals multiple regulators of caspase activation
... THEJOURNALOFCELLBIOLOGY JCB: REPORT Introduction Apoptosis is an evolutionally conserved cell death pathway that regulates development and tissue homeostasis. Caspases, a family of cysteine proteases, play a critical ...
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Genome-wide RNAi screen in Drosophila reveals Enok as a novel trithorax group regulator
... vivo genome-wide RNAi screen to identify potential trxG regulators in ...Our RNAi screen led to the discovery of more than 200 genes which perturbed our luciferase-based reporter ...
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A genome-wide RNAi screen identifies MASK as a positive regulator of cytokine receptor stability
... the enzymatic activity of -gal measured in the original screen (i.e. a 25% change in protein levels would not necessarily relate to a 25% change in luciferase values). Given the changes in Dome dimerisation and ...
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A quantitative genome-wide RNAi screen in C. elegans for antifungal innate immunity genes
... complete RNAi library contained a further 155 clones that potentially hit the same 404 targets (Additional file 5: Table ...the screen (360/(360 + 155), 70 %); in common with other RNAi screens ...
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Genome-wide RNAi Screen Reveals a Role for Multipass Membrane Proteins in Endosome-to-Golgi Retrieval
... a genome- wide RNAi loss-of-function screen for novel regula- tors of this trafficking pathway and report 90 genes that are required for endosome-to-Golgi retrieval of a CD8-CIMPR reporter ...
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Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila
... ISC screen (Table S1F), whose knockdown in ISCs (compare Figure S3B with Fig- ure S3A; Figures S3C and S3D), but not in EBs (compare Fig- ure S3F with Figure S3E; Figures S3G and S3H), resulted in a significant ...
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Reliance of Wolbachia on High Rates of Host Proteolysis Revealed by a Genome-Wide RNAi Screen of Drosophila Cells
... and genome-wide RNA interference (RNAi) screening to identify host factors that in fl uence Wolbachia ...an RNAi library targeting 15,699 transcribed host genes, we identi fi ed 36 candidate ...
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Integrative bioinformatics analyses of genome-wide RNAi screens
... Acknowledgements Although my name may appear on first but there have been substantial non-textual influences and contributions from a lot of people, that made this thesis possible. First and foremost, I would like to ...
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Genome-Wide RNAi Screen Reveals a Specific Sensitivity of IRES-Containing RNA Viruses to Host Translation Inhibition
... To dissect the cellular requirements for DCV replica- tion, we undertook a genome-wide screen in tissue cul- ture for host factors that, when lost, block viral infec- tion. Using this strategy we ...
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A co culture genome wide RNAi screen with mammary epithelial cells reveals transmembrane signals required for growth and differentiation
... understanding the pathophysiology of mammary epithelia, yet they remain poorly characterized. In this study, we applied an unbiased approach to understanding the functional role of signalling molecules in several models ...
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A Genome-Wide RNAi Screen for Enhancers of par Mutants Reveals New Contributors to Early Embryonic Polarity in Caenorhabditis elegans
... simple RNAi depletion screens have identified additional conserved regulators of polarity in ...a genome-wide RNAi screen for enhancers of lethality in conditional par-1 and par-4 ...our ...
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Genome-wide RNAi screen reveals the E3 SUMO-protein ligase gene SIZ1 as a novel determinant of furfural tolerance in Saccharomyces cerevisiae
... Results: By using a genome-wide RNAi (RNA-interference) screen in S. cerevisiae for genes involved in furfural tolerance, we identified SIZ1, a gene encoding an E3 SUMO-protein ligase. ...
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Genome-wide screen for modifiers of Parkinson's disease genes in Drosophila
... We then tested if the penetrance and severity of above phenotypes could be enhanced by increasing the expres- sion level of the UAS-park-RNAi transgene. This was achieved by elevating temperature, which increases ...
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Genome-Wide Analysis of Self-Renewal in Drosophila Neural Stem Cells by Transgenic RNAi
... our screen, most of these genes caused underproliferation and loss of Nbs, and this might indicate a role for alternative splicing in Nb ...individual RNAi lines resulted in divergent ...different ...
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Genome-wide suppressor screen identifies USP35/USP38 as therapeutic candidates for ciliopathies
... a genome-wide RNAi (RNA interference) screen to identify genetic suppressors of BBS4, one of the genes mutated in Bardet-Biedl syndrome ...
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Genome-Wide Screen for Inner Nuclear Membrane Protein Targeting in Saccharomyces cerevisiae
... the collections in a cell biology study to understand and the proteins with ectopic sequences may not achieve the appropriate structure. The latter possibility is consis- mechanisms by which nuclear proteins achieve ...
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Characterizing protein interactions employing a genome-wide siRNA cellular phenotyping screen
... gene pairs resulted in low or high performance of this classifier, respectively. This performance feature was employed together with an elaborated set of further features by a second set of classifiers (SVMs) ...
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Advances in genome-wide RNAi cellular screens: a case study using the Drosophila JAK/STAT pathway
... For screening, Drosophila Kc 167 cells were grown to al- most confluence in T-75 flasks and were then passaged into 9xT-75 flasks at a density of 40 million cells per flask and allowed to recover overnight. Cells were ...
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A genome-wide CRISPR screen identifies a restricted set of HIV host dependency factors
... the screen endpoints and the criteria for determining candidate genes also differed ...the RNAi-based screens were evaluated in an arrayed format 1–2 days after HIV infection, when weakly protective hits or ...
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Genome-Wide Screen Reveals Valosin-Containing Protein Requirement for Coronavirus Exit from Endosomes
... this genome- wide RNAi screen against IBV, we have identified 83 new cellular ...our screen, the host factors identified are likely to be conserved across species and, to some extent, ...
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