Making and sustaining the second person in an actor – the character on stage – so that he or she is acceptable and believable to the audience, while still remaining oneself is one of the most complicated things required of an actor. Using Stanislavsky’s method acting and techniques, he directs the actors using the following; objectives of each scene, line of actions and the super-objective of the play, analysis of text through action, truth, belief, imagination, subtext, motivation, concentration, relaxation, communion – communication with audience – adaptation, tempo-rhythm and the physical apparatus and movements (Downs & Ramsey, 2012). Murungu, thus, uses the auteur principles which regard the director as the single most important creative force in a play. According to this theory, the script alone is not sufficient to achieve the intended vision of the play. This means that the aforementioned skills and tools are vital in ensuring the director is able to make a play successful at achieving its vision (Innes & Shetsova, 2013). In light of this, Murunguwas highly successful at employing his improvisational style in directing his team of actors to bring out the intended meaning of the play “Torrent of Rodents”.
I am often struck, for instance, by the contrast between the fastidious details observed by designers and makers of clothing for Original Prac- tices productions and the relative lack of attention paid to the interaction between the clothes they make and the bodies of the actors for which they are made. By contrast, Chekhov’s process of imagining another body for the character offers the actor an opportunity to consider, for instance, the psychophysical effect of a ruff which radically separates head from trunk (embodying Cartesian dualism, perhaps) or of a fully-fastened doublet around the chest. Doublets are commonly worn open as though they were leather jackets which belong, in terms of acting, to twentieth century American realism and its emphasis on the free expression of personal feelings: the wearers’ hearts spill out of them. Thus, by wearing the clothes but ignoring the cultural practices, period drama is reduced to fancy dress. Chekhov’s imaginary body enables an actor to take the opposite approach and to explore the psychophysical consciousness of historical characters through analysis, understanding and embodiment of their material culture.
in the coming years. Nonetheless, access to these newly licensed HCV medicines remains a challenge in the Region due to the lack of a structured public health approach for prevention, diagnosis, treatment, and care of chronic hepatitis C. Among the challenges in expanding access to treatment are the absence of up-to-date and standardized care and treatment guidelines, lack of inclusion of new medicines in national essential medicine lists and formularies, and the elevated costs of direct-acting antiviral drugs (8).
Another aspect of the challenge casting children presents (especially in children’s theatre and/or school play) is that some children are indecisive in nature. They might show lack of interest in certain activities at the beginning only to come back later to express their interest in those same activities they were apathetic to initially. This was what the director encountered when directing Moremi. After casting those who were interested, the children who showed apathy initially came later to express interest in the activities they rejected earlier. Furthermore, some children were not quite sure where their interest was. Some wanted to act and were given roles. However, when they saw their friends dancing (in the same production), they ran to join the dancers, and after a while, they ran back to join the acting group again. Those who were dancers did the same thing. Moreover, some children swapped roles between themselves without the knowledge of the director. For instance, the child who finally played the character, Olorogbo, was not cast by the director. He did not even come out to audition for any role because he was timid initially. While alone with his friend who was initially cast for the role, he collected the script and read it and then afterwards begged his friend to give him that role and his friend obliged him. Surprisingly, he played the role very well.
This paper proposes a novel strategy for the design in synthesis of mesoporous materials with the use of swellable polymer networks used as scaffolds. Previous reports use different materials that have a templating effect towards the precur- sor. In such cases the organic material acts as central formation about which the precursor moieties organize and by this way a material influenced by the tem- plate is formed . In this paper, we show a novel path by using a removable scaffold used both as a structure and surface texture directing agent, influencing the resulting material and at the same time acting as a reaction vessel.
In this study, we develop a strategy to direct Bcr- Abl from the cytoplasm into the nucleus by induction of protein heterodimerization of FK506 binding protein (FKBP) and FKBP-rapamycin binding domain (FRB) via AP21967 [16-18]. FKBP is abundant in cytoplasm and serves as the target for rapamycin. Rapamycin functions by binding with high affinity to FKBP, and then to the FRB, thereby acting as a heterodimerizer to facilitate the binding of the two proteins . To use rapamycin for inducing heterodimers between proteins of interest, one of the two proteins is fused to FKBP, and the other to FRB, allowing sufficient binding to form the FKBP-rapamycin-FRB complex. Because rapamycin is an immunosuppressive reagent, chemically modified derivatives of rapamycin with non-immunosuppressive function have been engineered. These compounds, which are called rapalogs, can no longer bind to endogenous FRB, but can still bind to a modified FRB that contains a single mutation (T2098L).
In terms of chirality in gels, mixing enantiomers, both of which are independently capable of gelation, has been of considerable interest [9–12]. This is a special case of a Type 2 multi-component gel. In the vast majority of cases, enantiopure gelators assemble better than their racemic analogues [56–58]—i.e., homochiral interactions are normally favoured over heterochiral ones. However, in a few exceptional cases, heterochiral racemates show enhanced gelation [59–66]. This can be assigned to a thermodynamic preference for heterochiral interactions—either at the molecular- or nanoscale. Chiral directing effects have also been reported in which a small amount of chiral additive can impose its preference onto an achiral system (a “sergeants and soldiers” effect), or a small excess of one enantiomer can impose its preference onto the other (a “majority rules” effect) [67–74].
Example 1.6 (Group acting on itself by conjugation). Every group G acts on itself in a different way. Again set G = G and S = G. Then for g ∈ G and x ∈ S we define g.x = gxg −1 ∈ S = G. It is clear that this rule satisfies condition (0). Likewise (1) is easy since e.x = exe −1 = x. Finally we come to (2), and so we suppose that g, h ∈ G and x ∈ S = G. Then
8. The Plan is a product of extensive collaboration and consultation between Member States and PASB. A Member States Countries Consultative Group, appointed by PAHO’s Executive Committee (EC), provided strategic and technical input in the crafting of the Plan and its first Program and Budget (PB 2014-2015). National consultations were also conducted, enabling all countries and territories to provide their input to the documents to be presented to the Directing Council. More than 1,100 health professionals participated from 48 countries and territories across the Region. Member States and PASB will work jointly over the next six years to deliver the results set forth in the Plan. 9. The Plan responds to both regional and global mandates. As a result, its strategic agenda represents a balance between PAHO’s response to the regional priorities established in the Health Agenda for the Americas 2008-2017, other regional mandates set by PAHO Member States, the collective national priorities identified in PAHO’s Country Cooperation Strategies (CCS), and the programmatic alignment with the General Programme of Work (GPW) of the World Health Organization (WHO). The Plan is also consistent with the parameters of the main intergovernmental agreements for development operations of the United Nations system. The development and implementation of the Plan are guided by PAHO’s vision, mission, values, and core functions.
those of Mrs. F. This does not imply that deluded per- sons experience themselves as effective with respect to all aspects of their behaviour. They are usually unable to convince others about the trueness of their convictions. Furthermore, they are often ineffective in achieving goals and applying strategies directly deriving from, or relying upon, their delusional convictions. They are, however, typically able to achieve an explanation for the changes in their world. In this way, their freedom of intentional action is (in addition to symptom-related impairments of their free will) specifically altered. Persons with delu- sions are, as the case report of Mrs. F. most clearly dem- onstrated, able to experience themselves as free and self- determined in certain situations and actions; and they do in fact behave autonomously (and responsibly in a personal and legal sense) when taking into account the goods of agency in the situations in which they are called upon to act adequately. This is not denying the possibility of intentionally acting immorally, although the others’ goods of agency are accepted in principal and could be granted adequately. As has been argued above, such an adequateness is even possible if acting on one’s delusions. Although this need not be the case, and might seldom be, it seems to be especially possible if this person maintains a double-orientation to reality, implying that she discloses her life-world in two ‘parallel’ or ‘each other alternately eclipsing’ manners of familiar workspaces.
The synthesis of high-silica molecular sieve SSZ-70 is investigated through a guest/host study of imidazolium structure directing agents (SDAs). The original borosilicate synthesis is extended to pure-silica and aluminosilicate compositions using six imidazolium SDAs. Physical characterization using powder X-ray diffraction (XRD), 29 Si solid-state NMR, electron microscopy, thermogravimetric analysis, and nitrogen adsorption shows SSZ-70 to be layered with similarity to MCM-22 (MWW). Aluminum-containing SSZ-70 is evaluated for catalytic activity using the constraint index (CI) test and shows a similar cracking rate to SSZ-25 (MWW structure). Distinct differences in CI as a function of time on stream are observed between MWW and SSZ-70 materials. Additional molecular sieve phases observed in this guest/host study included Theta-1 (TON), ZSM-5 (MFI), ZSM-23 (MTT), ZSM-12 (MTW), Beta, Mordenite (MOR), CIT-5 (CFI), SSZ-16 (AFX), and SSZ-35 (STF).
The central theme of this thesis is the use of imidazolium-based organic structure directing agents (OSDAs) in microporous materials synthesis. Imidazoliums are advantageous OSDAs as they are relatively inexpensive and simple to prepare, show robust stability under microporous material synthesis conditions, have led to a wide range of products, and have many permutations in structure that can be explored. The work I present involves the use of mono-, di-, and triquaternary imidazolium-based OSDAs in a wide variety of microporous material syntheses. Much of this work was motivated by successful computational predictions (Chapter 2) that led me to continue to explore these types of OSDAs. Some of the important discoveries with these OSDAs include the following: 1) Experimental evaluation and confirmation of a computational method that predicted a new OSDA for pure-silica STW, a desired framework containing helical pores that was previously very difficult to synthesize. 2) Discovery of a number of new imidazolium OSDAs to synthesize zeolite RTH, a zeolite desired for both the methanol-to-olefins reaction as well as NO X reduction in
Thesis DIRECTING CELLULAR TRAFFIC USING GEOMETRIC AND BIOMOLECULAR CUES Thesis by Keiichiro Kushiro In partial fulfillment of the requirements for the degree of Doctor of Philosophy CALIFORNIA INSTITU[.]
two-step transcription amplification system, which comprised p β -Gal4-p65 and plasmid DNA system up-stream activating sequence (pUAS)-signal peptide (SP)-exenatide, combining the advantages of SP to facilitate its secretion in ectopic cells or tissue. Arginine-grafted cyctaminebisacrylamide- diaminohexane polymer (ABP) was employed as the gene carrier. Compared with 25K polyetherimide (PEI), the exenatide expression in the cells treated with 25K PEI/ TSTA-SP-exenatide was 1.4-fold lower than that of cells treated with ABP/TSTA-SP-exenatide. The glucoregulatory effect of ABP/TSTA-SP-exenatide showed a long-acting mode, lasting from the third day post-injection to ∼ 12 days post-injection.
Nicotine synthesis requires the concerted regulation of the metabolic pathways leading to both the production of nicotinic acid and to N-methylpyrrolinium (Feth et al., 1986; Wagner et al., 1986c). PMT and QPT are of great importance in controlling the supply of these two precursors in their synthesis pathways and they might be regulated coordinately. Previous studies substantiate this prediction. Both of them are under the control of the Nic loci; both are expressed dominantly in the roots; the transcripts level of both genes is up-regulated by wounding and topping, and down-regulated by auxin. Comparative studies of their cis-acting elements, which control the temporal and spatial expression of their downstream genes by interacting with particular trans-acting factors, likely would be profitable. We already have a lot of information about cis-acting