Adenoviral vectors
Improved Gene Delivery to Intestinal Mucosa by Adenoviral Vectors Bearing Subgroup B and D Fibers
... replication-incompetent adenoviral vectors, derived from human adenoviruses of subgroup C, are poorly efficient in delivering genetic material to differentiated intestinal ...suitable adenoviral ...
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Mesenchymal stem cell-mediated delivery of therapeutic adenoviral vectors to prostate cancer
... agarose. Spheroids were allowed to solidify for 48 h at 37 C in 5% CO2. Compacted spheroids were collected and washed with PBS. Then, these spheroids were implanted in the dorsal skinfold chambers in nude mice as previ- ...
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Mück-Häusl, Martin Andreas (2011): Genetic engineering of adenoviral vectors for improved therapeutic applications. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... modified adenoviral vectors, modulation of surface domains of the hexon protein might affect the total charge of the capsid surface and in consequence alter binding ...HCA vectors presented in this ...
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Innate Immune Response to Adenoviral Vectors Is Mediated by both Toll-Like Receptor-Dependent and -Independent Pathways
... Recombinant adenoviral vectors have been widely used for gene therapy applications and as vaccine vehicles for treating infectious diseases such as human immunodeficiency virus ...of adenoviral ...
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Oral tolerization to adenoviral antigens permits long term gene expression using recombinant adenoviral vectors
... of adenoviral vectors is limited by both humoral and cell- mediated antiadenoviral responses that limit the duration of expression transgenes introduced in vivo via adenoviral vec- ...
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Local delivery of adenoviral vectors encoding murine interleukin 10 induces colonic interleukin 10 production and is therapeutic for murine colitis
... 5 adenoviral vectors, encoding murine IL-10 under the transcriptional control of the rous sar- coma virus promoter (AdvmuIL-10), β-galactosidase under the cytomegalovirus promoter (AdvβGal), or having no ...
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Recombinant Adenoviral Vectors Can Induce Expression of p73 via the E4-orf6/7 Protein
... following adenoviral transduction: serum starvation, a pharmacological CDK inhib- itor, and a genetically encoded CDK ...nor adenoviral DNA replication is responsible for induction of TAp73 and ...
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Adenoviral Vectors in Veterinary Vaccine Development: Potential for Further Development
... AdV vectors. The vectors can be constructed to be replication competent or replication defective with most viral vectors available in both ...defective adenoviral vectors are preferred ...
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Molecular and macromolecular alterations of recombinant adenoviral vectors do not resolve changes in hepatic drug metabolism during infection
... None of the modifications commonly made to reduce the immunogenicity and toxicity associated with adenoviral vectors completely resolved aberrations in hepatic CYP. Administration of helper-dependent ...
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Rauschhuber, Christina (2011): Analysis of Adenovirus-Host Interactions to Improve Recombinant Adenoviral Vectors for Gene Therapy. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... generation adenoviral vectors (fgAd) (McCaffrey et ...recombinant adenoviral vectors for shRNA delivery against HBV, but also prototype foamy virus (Moore et ...
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Rapid Construction of Adenoviral Vectors by Lambda Phage Genetics
... of adenoviral vectors require the investigation of novel genome ...recombinant adenoviral cosmids during a productive lambda infection; (iii) transducing a recombination- deficient ...efficiently ...
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Improved vaccine protection against retrovirus infection after co administration of adenoviral vectors encoding viral antigens and type I interferon subtypes
... of adenoviral vectors encoding IFNa2, IFNa4, IFNa6 or IFNa9, but not IFNa1, IFNa5 or IFNb, resulted in significant reduction of FV-induced spleno- megaly (P < ...subtype vectors was confirmed when ...
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Immunogenicity and Cross-Reactivity of Rhesus Adenoviral Vectors
... most adenoviral vectors developed for vaccination have been HuAds from species B, C, D, and E, and human populations display moderate to high levels of preexisting ...new adenoviral vectors ...
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Induction of Immunological Tolerance to Adenoviral Vectors by Using a Novel Dendritic Cell-Based Strategy
... purified CD4 ⫹ and CD8 ⫹ T cells (21). IL-10 has been further shown to inhibit upregulation of CD86 on DCs. Addition of IL-10 to in vitro cultures of skin-derived dermal DCs resulted in signif- icant reduction of CD86 ...
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Efficient and Selective Gene Transfer into Primary Human Brain Tumors by Using Single-Chain Antibody-Targeted Adenoviral Vectors with Native Tropism Abolished
... exposure of cells deficient in the target molecule nor use of AdV targeted with irrelevant bispecific scFv led to consider- able transduction. The gene transfer efficiencies obtained with vectors with specificity ...
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Efficient Generation of Recombinant Adenoviral Vectors by Cre-lox Recombination In Vitro
... Using the Cre-loxP reaction, we prepared full- length recombinant adenoviral DNA in vitro by recombination between a shuttle plasmid and adenovirus genomic DNA that was previously trunca[r] ...
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The roles of adenoviral vectors and donor DNA structures on genome editing
... Providing a cure for monogenetic diseases, not by means of administering protein replacement therapies, but by gene complementation or even gene repair instead, is a long sought goal of gene therapy. Some of these ...
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Subretinal gene delivery using helper-dependent adenoviral vectors
... devoid of all viral coding genes, leaving only the ITR and ψ sequences necessary for vector replication and packaging, respectively [17,18]. This strategy prevents the production of any viral proteins which in turn sig- ...
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Original Article Targeting eradication of chronic myeloid leukemia using chimeric oncolytic adenovirus to drive IL-24 expression
... moter are also expressed in the tumor cells and against uncontrolled tumor cells. In the present study, most importantly, human adenovirus serotype 11 (Ad11) with a fiber different from that of Ad5 can entry cells which ...
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Unnatural Amino Acid Incorporation onto Adenoviral (Ad) Coat Proteins Facilitates Chemoselective Modification and Retargeting of Ad Type 5 Vectors
... adenovirus vectors can improve tissue-selective targeting, attenuate immunogenicity, and enable imaging of particle biodistribution, thus significantly improving therapeutic ...
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