Adipose-derived stromal cell
Improved GMP compliant approach to manipulate lipoaspirates, to cryopreserve stromal vascular fraction, and to expand adipose stem cells in xeno-free media
... years, stromal cells have been utilized in a growing number of trials for different clinical applications ...SVF derived from adipose tissue is an abundant source of ASC [7, ...Ex-vivo cell ...
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Investigation of Chitosan-based Hydrogels as a Cell Delivery Platform for Adipose-derived Stem/Stromal Cell Transplantation to Promote Angiogenesis in Ischemic Tissues
... the cell adhesion glycoprotein integrin β3 (ITGB3) also demonstrated a >2-fold enhancement in gene expression in the hydrogel groups relative to the TCPS ...4 cell donors for the secreted factors ...
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Mechanical loading of adipose derived stromal cells causes cell alignment
... of adipose derived stromal cells (ADSC) in vitro offers a potential solution for the treatment of cartilage de- fects that will allow patients to return to the physical activities they were involved ...
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Cellular therapy with human autologous adipose-derived adult cells of stromal vascular fraction for alopecia areata
... from adipose tissue [20], we characterized these freshly isolated ...1). Cell viability, as assessed by Trypan blue and validated by 7AAD stain- ing, was > 96% and cells affected by an early apoptosis ...
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Adipose tissue-derived stromal vascular fraction in regenerative medicine: a brief review on biology and translation
... population doublings in culture [1].The case of CD34 is interesting since it is still largely considered to be a marker for HSCs owing to its historical association with the enrichment of such cells for bone marrow and ...
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Safety of adipose-derived cell (stromal vascular fraction – SVF) augmentation for surgical breast reconstruction in cancer patients
... Background. Progress in breast cancer surgery results in a decreased frequency of mastectomy, in the early phases of cancer replaced by breast conserving therapy (lumpectomy). Increased popularity of breast ...
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Culture expansion of adipose derived stromal cells A closed automated Quantum Cell Expansion System compared with manual flask based culture
... One of the major risks related to the production of ASCs as well as any other cell product intended for clini- cal use is microbial contamination. Proper quality man- agement and aseptic manufacturing is the focal ...
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Extracellular vesicles for acute kidney injury in preclinical rodent models: a meta-analysis
... mesenchymal stromal cell (BMSC)-EVs [18, 27, 32, 40, 46–48], 6 used human umbilical cord mesenchymal stromal cell (UCMSC)-EVs [24, 28, 29, 36, 43, 44], 4 used human umbilical Wharton’s jelly ...
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Transplantation of modified human adipose derived stromal cells expressing VEGF165 results in more efficient angiogenic response in ischemic skeletal muscle
... Histological analysis of ischemic muscle injected with modified VEGF-ADSC revealed that capillary density was significantly higher than in specimens from animals that received untreated cells or GFP-ADSC. We noticed that ...
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Autologous adipose-derived stromal vascular fraction and scarred vocal folds: first clinical case report
... future cell therapy in laryngeal pathologies such as scarred VF where available treatments are ...of cell-based therapy products currently being developed, the ADSVF may have significant advantages because ...
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Therapeutic applications of adipose cell-free derivatives: a review
... require cell separation and in vitro cultivation and can be quickly prepared in the operating room or simple laboratory, significantly reducing the risk of biological contamin- ...medium derived from ...
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Extracellular vesicles derived from mesenchymal stromal cells: a therapeutic option in respiratory diseases?
... development, cell survival, and differenti- ation, while some MSC-derived EVs-enriched miRNAs are more closely associated with regulation of the immune system ...sources: adipose-derived MSCs ...
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Adipose-derived stromal cells in regulation of hematopoiesis
... regeneration, proliferation, and differentiation abilities of ASCs. The freshly isolated and expanded ASCs displayed several distinctions in biomarkers, gene expression, and biological properties (e.g. secretion of ...
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Are mesenchymal stromal cells immune cells?
... that adipose tissue-derived MSCs ex- press the haematopoietic stem cell marker ...are derived from MSCs and like their precursor cells they can adapt immunoregulatory properties and be- come ...
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Placenta-derived multipotent mesenchymal stromal cells: a promising potential cell-based therapy for canine inflammatory brain disease
... mesenchymal stromal cells (also known as mesenchymal stem cells; MSCs) derived from the bone marrow, adipose tissue, and birth-associated tissues, in- cluding umbilical cord, blood cord, amniotic ...
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Intra articular injection in the knee of adipose derived stromal cells (stromal vascular fraction) and platelet rich plasma for osteoarthritis
... Surgical methods of repair include the transplantation of osteochondral grafts, microfracture, and autologous chondrocyte implantation. All of these techniques are limited to the repair of focal lesions. According to ...
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Identical effects of VEGF and serum deprivation on phenotype and function of adipose derived stromal cells from healthy donors and patients with ischemic heart disease
... between poor viability and CD45 positivity could be due to unspecific CD45 labeling of dead and thereby permeable cells. Dead cells did not interfere with results for other markers. Taking this into consideration, ...
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Effects of platelet-rich plasma on the activity of human menstrual blood-derived stromal cells in vitro
... for cell prolifera- tion and wound healing ...stem cell-based therapies ...increase cell biological ...as adipose tissue-derived stem cells (ADSCs), BM-MSCs, and tendon stem cells have ...
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The ratio of ADSCs to HSC-progenitors in adipose tissue derived SVF may provide the key to predict the outcome of stem-cell therapy
... autologous stromal vascular fraction containing a rich mixture of various regenerative cell lines can be easily isolated in the operating room and redeployed back into patients during the same proce- dure ...
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Human cord blood-derived platelet lysate enhances the therapeutic activity of adipose-derived mesenchymal stromal cells isolated from Crohn’s disease patients in a mouse model of colitis
... Mesenchymal stromal cells (MSCs) are multipotent adult stem cells [1] which can differentiate in vitro and in vivo into several tissue lineages originating from the three ger- minal layers [2, ...stem cell ...
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