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Agrobacterium-mediated Genetic Transformation

Agrobacterium mediated Genetic Transformation in Cucumber (var  Shital) as Influenced by Explant, Inoculation Time and Co cultivation Period

Agrobacterium mediated Genetic Transformation in Cucumber (var Shital) as Influenced by Explant, Inoculation Time and Co cultivation Period

... of Agrobacterium mediated genetic transformation of potato Begum (2005) [5] found that both number and percentage of survived calli per pertridish were higher ...

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The roles of bacterial and host plant factors in Agrobacterium mediated genetic transformation

The roles of bacterial and host plant factors in Agrobacterium mediated genetic transformation

... nopaline-specific Agrobacterium strain (Citovsky et ...model, Agrobacterium- mediated genetic transformation with very long (about 150 kb) segments of DNA is enhanced by expressing ...

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Efficient method for Agrobacterium-mediated genetic transformation of tobacco nodal segments

Efficient method for Agrobacterium-mediated genetic transformation of tobacco nodal segments

... In the last decades, several methods have been developed and changes incorporated in the transformation process via A. tumefaciens, such as the addition of acetosyringone and changes in the periods of ...

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Molecular Cloning of Sucrose Isomerase Gene and Agrobacterium-Mediated Genetic Transformation of Potato (Solanum tuberosum L.) Plants

Molecular Cloning of Sucrose Isomerase Gene and Agrobacterium-Mediated Genetic Transformation of Potato (Solanum tuberosum L.) Plants

... respectively. The digested products were separated using agarose electrophoresis and the bands were extracted. The purified sucrose isomerase gene (palI) and the linear vector were ligated overnight at 16°C with T4 DNA ...

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Study of different factors affecting Agrobacterium- mediated genetic transformation in lentil (Lens culinaris Medik.)

Study of different factors affecting Agrobacterium- mediated genetic transformation in lentil (Lens culinaris Medik.)

... the Agrobacterium suspension overnight grown Agrobacterium culture taken from bacterial plate was centrifuged for 10 minutes at 5000 rpm and the pellet was re-suspended in liquid MS medium (pH ...This ...

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Standardization of Agrobacterium mediated genetic transformation in Indica rice cv BPT-5204

Standardization of Agrobacterium mediated genetic transformation in Indica rice cv BPT-5204

... of Agrobacterium tumifaciens virulence genes that regulate the processing and transfer of T- DNA from ...Higher transformation frequency is achieved by addition of acetosyringone in co-cultivation ...with ...

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Genetic transformation of tropical maize inbred lines using aldose reductase (ald1) gene for enhancement of drought tolerance

Genetic transformation of tropical maize inbred lines using aldose reductase (ald1) gene for enhancement of drought tolerance

... To enhance drought tolerance 111 locally adapted tropical maize lines through Agrobacterium-mediated transformation using XvALDl gene.u. 1. 4.2 Specific objective.[r] ...

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Assessment of plant transformation technology of maize

Assessment of plant transformation technology of maize

... problem, genetic engineering was ...increasing genetic resource to improve crop and also introduce known function of genes to achieve the goals of crop improvement by genetic engineering programme ...

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Development of agrobacterium mediated transformation protocol for mature seed derived callus tissues of citrus cultivar ‘gailiangcheng orange x weizhang satsuma mandarin’

Development of agrobacterium mediated transformation protocol for mature seed derived callus tissues of citrus cultivar ‘gailiangcheng orange x weizhang satsuma mandarin’

... low genetic diversity, apomixes, polyembryony, high heterozygosity, long juvenility, and auto-incompatibility (Boscariol et ...including genetic transformation have made it possible to overcome some ...

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EFFICIENT REGENERATION AND AGROBACTERIUM-MEDIATED TRANSFORMATION PROTOCOL FOR RECALCITRANT INDICA RICE (ORYZA SATIVA L.).

EFFICIENT REGENERATION AND AGROBACTERIUM-MEDIATED TRANSFORMATION PROTOCOL FOR RECALCITRANT INDICA RICE (ORYZA SATIVA L.).

... rice transformation protocol involved the use of embryogenic calli as the starting ...the transformation efficiency of indica rice varieties (Danilova, ...for genetic engineering is a crucial step in ...

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Transgenic Plant Production using Different T-DNA Technologies as a Tool of Genetic Engineering

Transgenic Plant Production using Different T-DNA Technologies as a Tool of Genetic Engineering

... T-DNA transformation by tissue culture with Agrobacterium mediated media in wild potato, potato, tomato, maize, apple, sweet orange, stawberry ...using Agrobacterium mediated ...of ...

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Agrobacterium-mediated transformation systems of Primula vulgaris

Agrobacterium-mediated transformation systems of Primula vulgaris

... for genetic analysis ...functional genetic assays allow rapid gene characterization with lower inputs than creating stable transformed plants, especially if the plants have long generation cycles like ...

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Agrobacterium Mediated Transformation of Maize (Zea mays L.)

Agrobacterium Mediated Transformation of Maize (Zea mays L.)

... a transformation frequency between 5- 30% for A188 line using a super-binary ...0% transformation frequency for A188 ...for genetic transformation of maize using ...

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Agrobacterium mediated Transformation of rice, var. Pusa Basmati-1

Agrobacterium mediated Transformation of rice, var. Pusa Basmati-1

... plants. Genetic engineering of rice enables breeders to design new varieties by the introduction of desired alien genes into existing commercial ...refining genetic transformation techniques in ...

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COMPARISON OF TRANSFORMATION EFFICIENCY OF FIVE AGROBACTERIUM TUMEFACIENS STRAINS IN NICOTIANA TABACUM L.

COMPARISON OF TRANSFORMATION EFFICIENCY OF FIVE AGROBACTERIUM TUMEFACIENS STRAINS IN NICOTIANA TABACUM L.

... Agrobacterium mediated transformation has been widely used for research in plant molecular biology and for genetic improvement of crops exploiting its tremendous ability to transfer foreign ...

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Evolution of Transgenic Technology in Cereal Crops: a Review Approach

Evolution of Transgenic Technology in Cereal Crops: a Review Approach

... Most genetic transformation approaches of cereal crops have been majorly restricted to rice, wheat, maize, barley and ...and transformation, modes of gene deliveries and use of suitable selection ...

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Tissue Culture-based Agrobacterium -mediated and in planta Transformation Methods

Tissue Culture-based Agrobacterium -mediated and in planta Transformation Methods

... of transformation also depends on the ability of selection procedure and the frequency of shoot regeneration and (pol- len or somatic) embryogenesis (Sobhanian et ...for genetic transformation of ...

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A High-Throughput Regeneration and Transformation Platform for Production of Genetically Modified Banana

A High-Throughput Regeneration and Transformation Platform for Production of Genetically Modified Banana

... Africa. Genetic engineering is a complementary option used for incorporating useful traits in banana to bypass the long generation time, polyploidy, and sterility of most of the cultivated ...robust ...

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High-Efficiency Agrobacterium-Mediated Transformation of Tobacco (Nicotiana tabacum)

High-Efficiency Agrobacterium-Mediated Transformation of Tobacco (Nicotiana tabacum)

... The Agrobacterium-mediated transformation is an efficient and low-cost tool that exploits the natural ability of ...The genetic transformation mediated by Agrobacterium ...

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Female reproductive system of Amaranthus as the target for Agrobacterium mediated transformation

Female reproductive system of Amaranthus as the target for Agrobacterium mediated transformation

... crops which can be found throughout the tropics and in many warm temperate regions [1]. Studies have been conducted to develop technologies that aim in exploiting Amaranthus due to its high nutritional value [2]. Tech- ...

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