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agrobacterium tumefaciens-mediated transformation

Agrobacterium tumefaciens-mediated transformation of Leptosphaeria app. and Oculimacula spp. with the reef coral gene DsRed and the jellyfish gene gfp

Agrobacterium tumefaciens-mediated transformation of Leptosphaeria app. and Oculimacula spp. with the reef coral gene DsRed and the jellyfish gene gfp

... Four filamentous ascomycetes, Leptosphaeria maculans, L. biglobosa, Oculimacula yallundae and O. acuformis, were transformed via Agrobacterium tumefaciens-mediated transformation with the genes ...

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Agrobacterium tumefaciens - mediated transformation of nicotiana benthamiana with dehalogenase gene resistant to monochloroacetic acid

Agrobacterium tumefaciens - mediated transformation of nicotiana benthamiana with dehalogenase gene resistant to monochloroacetic acid

... of Agrobacterium tumefaciens-mediated transformation were optimized including hygromycin concentration (40 μg/mL of hygromycin), and the MCA toxicity level to ...via Agrobacterium ...

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Agrobacterium tumefaciens-mediated transformation of Aspergillus aculeatus for insertional mutagenesis

Agrobacterium tumefaciens-mediated transformation of Aspergillus aculeatus for insertional mutagenesis

... adopting Agrobacterium tumefaciens-mediated transformation (AMT) for insertional mutagenesis; this method has been widely used as a genetic engineering technique for plant cells (Feldmann ...

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Agrobacterium tumefaciens Mediated Transformation of Wild Tobacco Species Nicotiana debneyi, Nicotiana clevelandii, and Nicotiana glutinosa

Agrobacterium tumefaciens Mediated Transformation of Wild Tobacco Species Nicotiana debneyi, Nicotiana clevelandii, and Nicotiana glutinosa

... on Agrobacterium tumefaciens-mediated transformation of wild tobaccos Nicotiana deb- neyi, Nicotiana clevelandii, and Nicotiana glutinosa were ...A. tumefaciens strain EHA105 carrying ...

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An improved Agrobacterium tumefaciens mediated transformation of Artemisia annua L  by using stem internodes as explants

An improved Agrobacterium tumefaciens mediated transformation of Artemisia annua L by using stem internodes as explants

... annua transformation system is of great ...of Agrobacterium tumefaciens mediated transforma- tion of ...A. tumefaciens growth, which is rather expensive and which has rather weak ...

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Integrative gene transfer in the truffle Tuber borchii by Agrobacterium tumefaciens-mediated transformation

Integrative gene transfer in the truffle Tuber borchii by Agrobacterium tumefaciens-mediated transformation

... plant transformation), be- tween the HindIII site and the right border of the T-DNA (Figure ...Tuber transformation and it was thus ...AGL-1/pABr3 Agrobacterium strains) revealed an approximately ...

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EARLY GFP GENE ASSESSMENTS INFLUENCING AGROBACTERIUM TUMEFACIENS-MEDIATED TRANSFORMATION SYSTEM IN PHALAENOPSIS VIOLACEA ORCHID

EARLY GFP GENE ASSESSMENTS INFLUENCING AGROBACTERIUM TUMEFACIENS-MEDIATED TRANSFORMATION SYSTEM IN PHALAENOPSIS VIOLACEA ORCHID

... component Agrobacterium (ii) the amount of inoculated bacteria that effectively bound to plant ...by Agrobacterium to plant cells has revealed two types of interactions: a nonspecific, non-saturable, ...

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Tetracycline Based Binary Ti Vectors pLSU with Efficient Cloning by the Gateway Technology for Agrobacterium tumefaciens Mediated Transformation of Higher Plants

Tetracycline Based Binary Ti Vectors pLSU with Efficient Cloning by the Gateway Technology for Agrobacterium tumefaciens Mediated Transformation of Higher Plants

... ABSTRACT We constructed small high-yielding binary Ti vectors with a bacterial tetracycline resistance gene to facilitate efficient cloning afforded by the Gateway Technology Invitrogen [r] ...

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Linkage Analysis of Transgenes Inserted into Cotton via Agrobacterium tumefaciens Transformation

Linkage Analysis of Transgenes Inserted into Cotton via <em>Agrobacterium tumefaciens</em> Transformation

... by Agrobacterium tumefaciens-mediated transformation is a documented technology in cotton (Firoozabady et ...germplasm. Transformation is considered to be a random event, with each ...

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A new vector system for targeted integration and overexpression of genes in the crop pathogen Fusarium solani

A new vector system for targeted integration and overexpression of genes in the crop pathogen Fusarium solani

... Background: Besides their ability to produce several interesting bioactive secondary metabolites, members of the Fusarium solani species complex comprise important pathogens of plants and humans. One of the major ...

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SUGARCANE GENETIC TRANSFORMATION MEDIATED BY AGROBACTERIUM TUMEFACIENS WITH MANNOSE AS SELECTION MARKER

SUGARCANE GENETIC TRANSFORMATION MEDIATED BY AGROBACTERIUM TUMEFACIENS WITH MANNOSE AS SELECTION MARKER

... As a new and safe positive selection marker, mannose did not harm to the growth of explants. Therefore, using mannose as selection marker could reduce the harmful influence on explant, and consequently higher ...

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Transformation of Tropical Maize with the NPK1 Gene for Drought Tolerance

Transformation of Tropical Maize with the NPK1 Gene for Drought Tolerance

... to Agrobacterium-mediated transformation with the NPK1 gene for conference of drought ...with Agrobacterium tumefaciens strain EHA101 harboring the pSHX004 ...to transformation ...

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Agrobacterium-mediated transformation systems of Primula vulgaris

Agrobacterium-mediated transformation systems of Primula vulgaris

... A. tumefaciens and assisting in the identification and removal of bacterial contaminated material prior to ...A. tumefaciens dur- ing co-cultivation ...stable transformation [30] The presence of ...

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COMPARISON OF TRANSFORMATION EFFICIENCY OF FIVE AGROBACTERIUM TUMEFACIENS STRAINS IN NICOTIANA TABACUM L.

COMPARISON OF TRANSFORMATION EFFICIENCY OF FIVE AGROBACTERIUM TUMEFACIENS STRAINS IN NICOTIANA TABACUM L.

... Agrobacterium mediated transformation has been widely used for research in plant molecular biology and for genetic improvement of crops exploiting its tremendous ability to transfer foreign DNA to ...

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TRANSGENIC LYCOPERSICUM ESCULENTUM MILL (TOMATO) PLANT AS BIOREACTOR FOR THE PRODUCTION OF HUMAN NEUTROPHIL PEPTIDE 1 (HNP 1): A USEFUL PROTEIN BASED PHARMACEUTICAL

TRANSGENIC LYCOPERSICUM ESCULENTUM MILL (TOMATO) PLANT AS BIOREACTOR FOR THE PRODUCTION OF HUMAN NEUTROPHIL PEPTIDE 1 (HNP 1): A USEFUL PROTEIN BASED PHARMACEUTICAL

... Hence they are acting as a natural bioreactor for producing therapeutic proteins and peptides in large amount. The aim of the present work was producing a Human Neutrophil Peptide-1 (HNP-1) which is an antimicrobial ...

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Agrobacterium mediated Transformation of rice, var. Pusa Basmati-1

Agrobacterium mediated Transformation of rice, var. Pusa Basmati-1

... Agrobacterium tumefaciens strain LBA 4404 (pIG121) was cultured on liquid LB medium [22] containing kanamycin sulfate (50 mg/l), hygromycin-B (50 mg/l) and agar (15 g/l) for 3 days at 28°C in the ...For ...

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EFFICIENT REGENERATION AND AGROBACTERIUM-MEDIATED TRANSFORMATION PROTOCOL FOR RECALCITRANT INDICA RICE (ORYZA SATIVA L.).

EFFICIENT REGENERATION AND AGROBACTERIUM-MEDIATED TRANSFORMATION PROTOCOL FOR RECALCITRANT INDICA RICE (ORYZA SATIVA L.).

... For the optimization study, the parameter to be investigated was varied while the others remained the same as the standard procedure. Five parameters (Agrobacterium strains, seedling age, co-cultivation period, ...

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OPTIMIZATION OF AGROBACTERIUM TUMEFACIENS MEDIATED GENETIC TRANSFORMATION FOR SYNTHESIS OF ANTI MALARIAL DRUG ARTEMISININ FROM PLANT ARTEMISIA ANNUA

OPTIMIZATION OF AGROBACTERIUM TUMEFACIENS MEDIATED GENETIC TRANSFORMATION FOR SYNTHESIS OF ANTI MALARIAL DRUG ARTEMISININ FROM PLANT ARTEMISIA ANNUA

... co-cultivation media as is presented in Table 8. As such there is no difference in induction frequency at any other respective O.D or explants. After the addition of hormone to co- cultivation medium, the frequency of ...

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Transformation of HBsAg (hepatitis B surface antigen) gene into tomato mediated by Agrobacterium tumefaciens

Transformation of HBsAg (hepatitis B surface antigen) gene into tomato mediated by Agrobacterium tumefaciens

... via Agrobacterium-mediated ...including Agrobacterium strains, infection time, co-cultivation time, Agrobacterium concentrations and acetosyringone concentrations were ...A. tumefaciens ...

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Journal of Applied Pharmaceutical Science

Journal of Applied Pharmaceutical Science

... the Agrobacterium research ...either Agrobacterium or plant ...for transformation, many Agrobacterium chromosomal genes are also essential for this ...A. tumefaciens C58 sequence will ...

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