AMPK signal pathway
Isoquercitrin activates the AMP–activated protein kinase (AMPK) signal pathway in rat H4IIE cells
... sodium pyrophosphate, 2 mM phenylmethanesulfonyl- fluoride (PMSF), 1 mM benzamidine, 0.02 g/mL trypsin inhibitor, 0.02 g/mL leupeptin, and 0.02 g/mL aprotinin. After incubation on ice for 30 min, the lysates were cen- ...
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Transcriptional Activation of Human GD3 Synthase (hST8Sia I) Gene In curcumin-Induced Autophagy in A549 Human Lung Carcinoma Cells
... Curcumin, a natural polyphenolic compound isolated from the plant Curcuma longa, is known to induce autophagy in various cancer cells, including lung cancer. In the present study, we also confirmed by LC3 ...
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Original Article Protective effects of berberine on high fat-induced kidney damage by increasing serum adiponectin and promoting insulin sensitivity
... sensor AMPK orchestrates the regulation of energy- generating and energy-consuming ...pathways. AMPK is highly expressed in the kidney where it is reported to be involved in a variety of physi- ological and ...
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ATP directed agent, 8-chloro-adenosine, induces AMP activated protein kinase activity, leading to autophagic cell death in breast cancer cells
... AMPK through its ability to decrease ATP levels. Metfor- min has been shown to suppress spontaneous tumor for- mation in various animal models as well as suppress in vitro and in vivo tumor growth. Moreover, ...
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Vol 68, No 3 (2016)
... Abstract: Evodiamine, a naturally occurring indole alkaloid, has been reported to have numerous biological activities, including antitumor, antimicrobial and anti-inflammatory effects. Previous studies also suggest that ...
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Wnt3a ligand facilitates autophagy in hippocampal neurons by modulating a novel GSK-3β-AMPK axis
... Total protein extraction from cultured hippocampal neurons and immunoblot analysis were performed as previously described [35]. The following primary anti- bodies were used: rabbit anti-pSer9-GSK-3β, anti-GSK- 3β, rabbit ...
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Original Article Pathway analysis of esophageal squamous cell carcinoma using iTRAQ-based quantitative proteomic approach
... Abstract: Purpose: Esophageal squamous cell carcinoma (ESCC) is one of the most common digestive cancers. Many proteins and genes have been found to be associated with ESCC. However, there is rare study on the pathways ...
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PCK1 negatively regulates cell cycle progression and hepatoma cell proliferation via the AMPK/p27Kip1 axis
... by AMPK through elevated intracellular AMP/ATP ...phosphorylated AMPK acts to restore the energy bal- ance by increasing catabolic ATP-generating processes, such as glycolysis and fatty-acid oxidation, ...
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Original Article Effect of metformin on Schwann cells under hypoxia condition
... Metformin, which is the first-line drug for the treatment of diabetes mellitus type-2 [1], has been proved to possess beneficial effects on nervous system in many studies. It has shown that metformin pretreatment could ...
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Therapeutics targeting CD90-integrin-AMPK-CD133 signal axis in liver cancer
... Integrins recognize arginine-glycin-aspartic acid (RGD) sequence and contribute to the cancer cell growth. The RGD-like sequence, RLD, also binds to integrin and participates in the signaling transduction. We sought to ...
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Original Article Adiponectin inhibits NLRP3 inflammasome by modulating the AMPK-ROS pathway
... Accumulating evidence has illustrated that adi- ponectin has positive actions on obesity-linked metabolic complications. Because of these beneficial effects, adiponectin has attracted tremendous scientific interest in ...
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Metformin reverses development of pulmonary hypertension via aromatase inhibition
... kinase AMPK in many tissues, although AMPK does not underlie the hypoglycaemic actions of ...22 AMPK is a ubiquitously expressed serine/threonine protein kinase, which plays a critical role in ...
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Effect of cold atmospheric plasma treatment on the metabolites of human leukemia cells
... leukemia cells so that glutamine was not able to be nor- mally metabolized to produce glutamic acid and thus accumulated, which might lead to leukemia cells death due to the lack of required nutrients. Our current work ...
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Original Article Metformin inhibits angiogenesis in endothelial progenitor cells through inhibiting MMP2, MMP9 and uPA expression via AMPK-mTOR-autophagy pathway
... Antibodies for phospho-AMPK, AMPK, LC3B were from Cell Signaling Technology (Danvers, MA, USA). Antibodies for urokinase-type plas- minogen activator (uPA), Metal Matrix Pro- teinase (MMP) 2, MMP9, Atg5 and ...
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Role of miRNAs in skeletal muscle aging
... muscle aging process involved with has-miR-19a. hsa-miR- 34a, with a degree of 217, was another significant node in the miRNA–target gene network. Pathway analysis indicated that has-miR-34a was significantly ...
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<p>Metformin Induces Autophagy via the AMPK-mTOR Signaling Pathway in Human Hepatocellular Carcinoma Cells</p>
... Our result that metformin could induce the autophagy of HCC cells was consistent with some previous published researches. 19,20 Tsai et al found that metformin could activate autophagy in Huh7 cells and the underlying ...
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Modulation of IGF-I Receptor Activation by the IGF-I peptides
... The EA peptide was shown to augment IGF-I potency, which is consistent with our findings9. These authors also found that the E-peptide-induced increase in IGF-IR activation did not alter the AKT/P13kinase pathway. ...
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Huhs1015 downregulates pkm2 through an ampk/p38mapk pathway in mkn45 cells
... activates AMPK, to downregulate PKM2: AMPK is activated by being phosphorylated at Thr172 (Russo et ...phosphorylates AMPK at Thr172 in a treatment time (1-10 min)-dependent manner in MKN45 cells ...
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Endothelin-1 promotes epithelial–mesenchymal transition in human chondrosarcoma cells by repressing miR-300
... Chondrosarcoma is a malignant tumor of mesenchymal origin predominantly composed of cartilage-producing cells. This type of bone cancer is extremely resistant to radiotherapy and chemotherapy. Surgical resection is the ...
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Original Article AMPK activity is down-regulated in endothelial cells of GHS-R mice
... that AMPK has the similar effects of Ghrelin/GHS-R that plays protective roles in AS by inhibiting the inflam- matory response as well as cell proliferation [17], however, no reported studies verify wheth- er ...
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