B cell epitope mapping
B Cell Epitope Mapping of the VapA Protein ofRhodococcus equi: Implications for Early Detection ofR equi Disease in Foals
... linear epitope of VapA lies in the region between peptides 11 and 14 corresponding to amino acids 62 to 81 of the VapA precursor protein ...a B-cell epitope is in this region of ...the ...
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Immunoinformatics Based Vaccine Design for Zea M 1 Pollen Allergen
... allergens. B-cell epitope mapping is a promising method to identifying the main antigenic determinants or epitopes of ...microorganisms. Epitope- based vaccines have outstanding ...
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Epitope mapping and characterization of a novel Nsp10 specific monoclonal antibody that differentiates genotype 2 PRRSV from genotype 1 PRRSV
... fine epitope mapping of mAb 4D9 using a panel of eukaryotic expressed polypeptides with GFP-tags, we identified the motif 286 AIQPDYRDKL 295 as the minimal unit of the linear B-cell ...
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Epitope Mapping Porcine Reproductive and Respiratory Syndrome Virus by Phage Display: the nsp2 Fragment of the Replicase Polyprotein Contains a Cluster of B-Cell Epitopes
... RKASLSTS epitope in the over- lapping ORF 3 ...RKASLSTS epitope was intact in 70% of Danish field PRRSV isolates from 1992, it is deleted in 63% of present-day Danish field PRRSV isolates ...cluster ...
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Identification of a novel linear B cell epitope using a monoclonal antibody against the carboxy terminus of the canine distemper virus nucleoprotein and sequence analysis of the identified epitope in different CDV isolates
... linear epitope by Pepscan analysis Twenty-three overlapping synthetic peptides (15aa) cor- responding to the coding sequence of recombinant N protein (aa 401–523) were synthesized (Sangon, China) to map the mab ...
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Arthritogenic T cell epitope in glucose 6 phosphate isomerase induced arthritis
... By epitope mapping, human GPI325–339 was found to induce interferon (IFN) γ and IL-17 production most ...led B cells to produce anti-mouse GPI antibodies, which were deposited on cartilage ...
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IN SILICO HOMOLOGY STRUCTURE, B CELL AND T CELL EPITOPES PREDICTION OF SERINE/THREONINE PROTEIN KINASE A AGAINST TUBERCULOSIS
... in cell division, cell differentiation and cell ...with B-cell epitope and T-cell mapping that highlights the immunogenic part of a ...as B-cell ...
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T cell subsets, epitope mapping, and HLA restriction in patients with allergic bronchopulmonary aspergillosis
... T cell responses and their role in the pathogenesis of ...T cell reactivity to Af antigens, T cell clones (TCC) specific to the Asp f 1 antigen, an 18-kD protein of Af, were established from the ...
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Computational Methods in Linear B cell Epitope Prediction
... linear B-cell epitopes, extracted from Bcipep database, and 701 non-epitopes, randomly extracted from ...length B-cell epitopes prediction models in two ...is mapping flexible length ...
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ANALYSIS OF HYDROPHOBICITY AND ANTIGENICITY OF HEAT SHOCK PROTEIN 70 FROM GWD
... antibody epitope from Heat shock 70 protein sequences with the consideration of the parameters such as hydrophilicity, flexibility, accessibility, turns, exposed surface, polarity and antigenic propensity of ...
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Recombination mediated escape from primary CD8+ T cells in acute HIV 1 infection
... T cell responses that dominantly target conserved regions of HIV-1 will therefore also be less subject to rapid escape by recom- bination suggesting a conserved immunogen design for prophylactic T cell ...
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Identification of B Cell Epitope of Dengue Virus Type 1 and Its Application in Diagnosis of Patients
... map B-cell epitopes, search for disease-specific antigen mimics, and ana- lyze conformational epitopes or mimotopes (6, 7, 8, 19, 22, ...Serotype-specific B-cell epitopes of DEN have not been ...
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Mapping an Adeno-associated Virus 9-Specific Neutralizing Epitope To Develop Next-Generation Gene Delivery Vectors
... Here, we report the cryo-EM reconstruction of AAV9 in complex with the highly potent and specific mAb PAV9.1. The epitope determined for PAV9.1 largely overlaps with the epitopic regions of other AAV NAbs isolated ...
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Mapping of B-cell epitopes of the human hepatitis B virus X protein.
... The carboxy-terminal half of the HBx antigen was the most frequent target for anti-HBx antibodies in sera of patients with viral hepatitis and contained an immunodominant antigenic regio[r] ...
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Presentation of an immunodominant T-cell epitope of hepatitis B surface antigen by the HLA-DPw4 molecule.
... Antibody DA6.231, with broad specificity for DR and DP, and antibody B7/21, specific for DP antigens, were tested for their capacity to inhibit the proliferative response of the HBsAg-sp[r] ...
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Identification of a conserved linear B cell epitope in the M protein of porcine epidemic diarrhea virus
... the epitope peptide did not react with TGE-positive serum, but it showed reactivity with PEDV-positive serum, indicating that epitope 195 WAFYVR 200 is specific for ...the epitope as ...
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Molecular basis of factor VIII inhibition by human antibodies Antibodies that bind to the factor VIII light chain prevent the interaction of factor VIII with phospholipid
... 1 5.1 Subsequent more detailed epitope mapping by deletion analysis ofrecombinant factor VIII protein fragments has suggested that light chain-specific inhibitors recognize an epitope in[r] ...
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Title: FORMULATION & EVALUATION OF TRANSDERMAL PATCH OF TOLBUTAMIDE Author: Ganju Eisha* and Ganju Kuldeep Keyword: Transdermal drug delivery, Matrix patch preparation, Membrane controlled Patch, tolbutamide, HPMC, PVP, In- vitro skin permeation studies.Page No: 01-04Abstract: The aim of the present investigation was to prepare tolbutamide transdermal patches by solvent casting technique using the HPMC and PVP polymer in different ratios. The systems were evaluated for var ious in- vitro parameters like (Thickness, Folding endurance, Moisture content, Moisture uptake, etc). In- vitro permeation studies were performed by using Franz diffusion cell. Variations in drug permeation profile were observed among various formulations. From all the formulations, formulation P4 was selected as the best formulation and formulation was stable for period of 90 days stability study. Keywords: Transdermal drug delivery, Matrix patch preparation, Membrane controlled Patch, tolbutamide, HPMC, PVP, In- vitro skin permeation studies. Download PDF
... Hybridomas are cells that have been built to deliver a coveted immunizer in expansive sums, to create monoclonal antibodies. (1, 2) Monoclonal antibodies can be delivered in particular cells through a method now ...
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Replacing adenoviral vector HVR1 with a malaria B cell epitope improves immunogenicity and circumvents preexisting immunity to adenovirus in mice
... a B cell epitope derived from a Plasmodium yoelii circumsporozoite (CS) protein (referred to as the PyCS-B epitope) into the capsid proteins of WT/CS-GFP, a recombinant Ad expressing ...
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Enhancement of the antibody response to flavivirus B-cell epitopes by using homologous or heterologous T-cell epitopes.
... DISCUSSION This is the first report of a biologically active flavivirus Th-cell epitope capable of providing help for a homologous flavivirus B-cell antibody response to another peptide [r] ...
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