Bispecific Antibody
<p>Suppression of c-Met-Overexpressing Tumors by a Novel c-Met/CD3 Bispecific Antibody</p>
... of antibody-based therapeutic strategies have been explored to block c-Met or HGF in cancers; however, clinical efficacy has been very limited, indicating that blockade of c-Met signal alone is not ...c-Met/CD3 ...
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The CTLA-4 x OX40 bispecific antibody ATOR-1015 induces anti-tumor effects through tumor-directed immune activation
... ADCC: Antibody-dependent cell-mediated cytotoxicity; ADCP: antibody- dependent cellular phagocytosis; bsAb: bispecific antibody; CHO: Chinese hamster ovary; Fc γ R: Fc γ receptor; GITR: ...
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Targeted cancer immunotherapy via combination of designer bispecific antibody and novel gene engineered T cells
... novel bispecific antibody against a TAA and the extracellular domain of the costimulatory immune-receptor, may circumvent some of the potential major limitations associated with infusing co-stimulatory ...
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Synergistic Neutralization of Pertussis Toxin by a Bispecific Antibody In Vitro and In Vivo
... the bispecific antibody can be assessed in a similar way, although avidity effects complicate the ...the bispecific antibody can be expected to retain all of the hu1B7 ...6 ...
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Bispecific Antibody Conjugated Manganese-Based Magnetic Engineered Iron Oxide for Imaging of HER2/neu- and EGFR-Expressing Tumors
... ly). Fig. 6 showed substantial variations of FITC fluo- rescence signals observed for SKBR-3, A431, and Co- lo-205 tumor cells at 37 °C. Although all three cells showed green fluorescence signals, the green fluo- ...
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A single-domain antibody-linked Fab bispecific antibody Her2-S-Fab has potent cytotoxicity against Her2-expressing tumor cells
... as diabodies, minibodies and tandem single-chain variable fragments (scFvs) (Muller and Kontermann 2007; Chames and Baty 2009). As ScFv based antibod- ies have the potential issues with poor stability; and tendency of ...
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Effects of different interchain linkers on biological activity of an anti-prostate cancer single-chain bispecific antibody
... recombinant antibody and parental antibody had almost the same affinity for the antigen and good anti-tumour ...single-chain antibody with good immunological competence with a linker composed of 25 ...
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Solitomab, an EpCAM/CD3 bispecific antibody construct (BiTE®), is highly active against primary uterine and ovarian carcinosarcoma cell lines in vitro
... other antibody/BiTE®-based therapies, may be effective through either IV or intraperitoneal/ intrapleural administration because of the frequency of ex- pression of EpCAM on the surface of CS combined with its ...
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Bispecific antibody targets multiple Pseudomonas aeruginosa evasion mechanisms in the lung vasculature
... compartments. Antibody-mediated inhibition of the T3S protein PcrV did not enhance bacterial phagocytosis but did enhance killing of the few bacteria ingested by ...A bispecific mAb targeting both Psl and ...
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A Bispecific Antibody That Simultaneously Recognizes the V2 and V3 Glycan Epitopes of the HIV 1 Envelope Glycoprotein Is Broader and More Potent than Its Parental Antibodies
... It has previously been reported that one V2-glycan antibody, PG9, can interfere with neutralization by V3-glycan antibodies in some circumstances (29). Similar interference may account for the inability of PGT145 ...
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A novel asymmetrical anti-HER2/CD3 bispecific antibody exhibits potent cytotoxicity for HER2-positive tumor cells
... engagers (BiTE) [8–10] and Dual-affinity Re-Targeting (DART) [11], have short half-lives in vivo and lack anti- body-dependent cell-mediated cytotoxicity (ADCC) ef- fect due to their low molecular weight and the absence ...
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A bispecific antibody recognizing influenza A virus M2 protein redirects effector cells to inhibit virus replication in vitro.
... of bispecific antibodies (BsAbs) to bypass the need for virus-specific MHC-restricted T cells to inhibit experimental virus ...the antibody re- sponse with the efficiency of the cellular immune ...
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Site-specific PEGylation of an anti-CEA/CD3 bispecific antibody improves its antitumor efficacy
... The 20 kDa linear methoxy PEG maleimide (MAL-PEG- OMe; Lot A3115) was purchased from JenKem Technology Co., Ltd. (Beijing, People’s Republic of China). Human colon adenocarcinoma cell line LS174T and human breast cancer ...
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Potential of the Trifunctional Bispecific Antibody Surek Depends on Dendritic Cells: Rationale for a New Approach of Tumor Immunotherapy
... Trifunctional bispecific antibodies (trAbs) used in tumor immunotherapy have the unique ability to recruit T cells toward anti- gens on the tumor cell surface and, moreover, to activate accessory cells through ...
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Engineering and characterising a novel, highly potent bispecific antibody iMab CAP256 that targets HIV 1
... immobilized G. nivalis lectin (Sigma) (200 ng/well) on a 96 well ELISA plate (Maxisorb—Nunc). To assess bind- ing to human CD4, decreasing concentrations of bacteri- ally expressed 2dCD4 was immobilized directly on the ...
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Purified anti-CD3 × anti-HER2 bispecific antibody potentiates cytokine-induced killer cells of poor spontaneous cytotoxicity against breast cancer cells
... Background: Chemical crosslinking is the most straightforward method to produce bispecific antibodies (BsAb) for arming ex vivo activated cytotoxic T lymphocytes. However, heterogeneous polymers are produced by ...
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Ipilimumab augments antitumor activity of bispecific antibody armed T cells
... monoclonal antibody (OKT3, Ortho Biotech, Horsham, PA) and ex- panded with 100 IU/mL of Interleukin-2 (IL-2) in RPMI-1640 supplemented with 10% FBS, 2 mM L- glutamine, 50 units/mL penicillin and 50 μg/mL strepto- ...
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Tandem bispecific neutralizing antibody eliminates HIV 1 infection in humanized mice
... potency, bispecific bnAbs (bs-bnAbs) have been engineered using the available gene sequences of bnAbs ...of antibody light and heavy chains ...CD3-targeting bispecific antibody for acute B The ...
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Enhancement of Adenovirus Vector Entry into CD70-Positive B-Cell Lines by Using a Bispecific CD70-Adenovirus Fiber Antibody
... Here we show that BsAb directed against the CD70 receptor and the adenovirus fiber protein (BsAb-CD70-fiber) signifi- cantly enhance adenovirus delivery to CD70-positive, but not CD70-negative, cell lines. By using this ...
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Recent advances of bispecific antibodies in solid tumors
... BiTE antibody me- diating T cell-directed cytotoxicity towards CEA positive tumor cells, was positively correlated with CEA antigen density regardless of the mutational status of the tumor cell lines, including ...
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