Bruker Biotyper
Comparison of Direct Colony Method versus Extraction Method for Identification of Gram Positive Cocci by Use of Bruker Biotyper Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
... the Bruker Biotyper system (Bruker Dal- tonics, Billerica, MA) and achieved spectra with mass ranges from 2 to 25 kDa and ⬎ 50 peaks for 10 different Gram- negative ...
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Bruker Biotyper Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System for Identification of Nocardia, Rhodococcus, Kocuria, Gordonia, Tsukamurella, and Listeria Species
... the Bruker Biotyper MALDI- TOF system for identifying aerobically growing Gram-positive rod-shaped bacilli that had been confirmed by molecular identi- fication methods disclosed several important ...the ...
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Evaluation of the Bruker Biotyper and Vitek MS Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Systems for Identification of Nonfermenting Gram Negative Bacilli Isolated from Cultures from Cystic Fibrosis Patients
... the Bruker system (20 of 52 isolates) in com- parison to Vitek MS (2 of 23 ...the Bruker system database and testing procedures for direct bacterial isolate testing may eventually eliminate the need for ...
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Evaluation of the Bruker Biotyper Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System for Identification of Blood Isolates of Acinetobacter Species
... the Bruker Biotyper in correctly identifying ACB com- plex isolates to the species level (score values ⱖ ...the Bruker Biotyper system, the correct identification rate was only ...the ...
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A Comprehensive Evaluation of the Bruker Biotyper MS and Vitek MS Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Systems for Identification of Yeasts, Part of the National China Hospital Invasive Fungal Surveillance Net (CHIF NET) Study, 2012 to 2013
... the Bruker Biotyper MS system, pure yeast isolates (each from a single colony), were directly smeared onto the target plate (Bruker Daltonics GmbH) and overlaid with 1 l of 70% formic acid ...by ...
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Comparison of the Bruker Biotyper and Vitek MS Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Systems for Identification of Mycobacteria Using Simplified Protein Extraction Protocols
... analysis: Bruker MALDI ...The Bruker bac- terial test standard (Bruker Daltonics), an extract of Escherichia coli sup- plemented with RNase A and myoglobin, was used for daily calibration according ...
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Comparison of Bruker Biotyper Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometer to BD Phoenix Automated Microbiology System for Identification of Gram Negative Bacilli
... After initial analysis on the Bruker Biotyper, any isolate that failed to produce a score of ⱖ 2.000 or generated a score of ⱖ 2.000 but failed the 10% differential rule was reanalyzed by the direct colony ...
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Optimization of Routine Identification of Clinically Relevant Gram Negative Bacteria by Use of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and the Bruker Biotyper
... the Bruker Biotyper MALDI-TOF MS system, including density of organism spotting on a stainless steel target plate and the direct overlay of organisms with formic ...criteria. Biotyper identifications ...
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Optimizing Identification of Clinically Relevant Gram Positive Organisms by Use of the Bruker Biotyper Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System
... Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) can be used as a method for the rapid identification of microorganisms. This study evaluated the Bruker Biotyper ...
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Identification of Anaerobic Bacteria by Bruker Biotyper Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry with On Plate Formic Acid Preparation
... the Bruker Biotyper using an off-plate extraction method produced correct species-level identification in 79% of isolates using a cutoff score of ⱖ ...the Bruker Biotyper system have shown ...
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Comparative Evaluation of the Bruker Biotyper and Vitek MS Matrix Assisted Laser Desorption Ionization–Time Of Flight (MALDI TOF) Mass Spectrometry Systems for Identification of Yeasts of Medical Importance
... LT Biotyper (Bruker Daltonics, Bremen, Germany) (BMS), the Sara- mis system (bio-Mérieux, Marcy l’Etoile, France), the Vitek MS system (bioMérieux, Marcy l’Etoile, France) (VMS), and, very re- cently, the ...
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Identification of Molds by Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
... the Bruker Biotyper database (OC version ...current Bruker Biotyper database, that were enclosed in 12 sections (mainly sections Circumdati, Nidulantes, Flavi, Terrei, Versicolores, ...
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Comparison of the Vitek MS and Bruker Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Systems for Identification of Chryseobacterium Isolates from Clinical Specimens and Report of Uncommon Chryseobacterium Infections in Humans
... The correct identification of pathogens is of paramount importance in clinical practice, epidemiological study, and the basic research of microorganisms. The use of 16S rRNA gene sequencing is considered the reference ...
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An Update on the Streptococcus bovis Group: Classification, Identification, and Disease Associations
... Over the last 5 years, MALDI-TOF MS has transformed the clin- ical microbiology and infectious diseases fields, enabling signifi- cantly faster time to identification at a discriminatory power that is considerably higher ...
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Comparison of the Vitek MS and Bruker Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Systems for Identification of Rhodococcus equi and Dietzia spp
... of Bruker Biotyper versus that of Vitek MS for identification of a collection of 154 iso- lates identified at the source as ...identification. Biotyper identified 131 ...
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Dermatophyte Identification Using Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
... the Bruker Biotyper matrix-assisted laser desorption ionization–time of flight (MALDI- TOF) mass spectrometer (MS) for the identification of dermatophytes from clinical cultures was compared to that of ...
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Advantages of Using Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry as a Rapid Diagnostic Tool for Identification of Yeasts and Mycobacteria in the Clinical Microbiological Laboratory
... Among the 11 different Mycobacterium species that were iden- tified by the Biotyper system, M. tuberculosis complex was success- fully differentiated from other NTM species, including 8 slowly growing mycobacteria ...
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Improved Differentiation of Streptococcus pneumoniae and Other S mitis Group Streptococci by MALDI Biotyper Using an Improved MALDI Biotyper Database Content and a Novel Result Interpretation Algorithm
... MALDI Biotyper database and using the new list(score) algorithm to interpret MALDI Biotyper results led to significantly improved differentiation of pneumococci from other species of the ...MALDI ...
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Yeast On Target Lysis (YOTL), a Procedure for Making Auxiliary Mass Spectrum Data Sets for Clinical Routine Identification of Yeasts
... MALDI Biotyper system, a multistep extraction protocol, which is also used to generate the reference spectra, is ...MALDI Biotyper log score values to allow automated species ...
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Improved Identification of Yeast Species Directly from Positive Blood Culture Media by Combining Sepsityper Specimen Processing and Microflex Analysis with the Matrix Assisted Laser Desorption Ionization Biotyper System
... 28S rRNA gene sequencing. Specimens with discrepant identification results between the phenotypic and MALDI Biotyper methods were further identified by sequencing analysis. A primer set (28S-Haynes-40F [5⬘-GCA TAT ...
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