CD34 Stem cells
Comparison between the Effect of CD34+ Stem Cells and Mesenchymal Stem Cells in Improving Pulmonary Fibrosis in Bleomycin Rat Model
... which stem cells ameliorate bleomycin-induced PF in rats may involve the inhibition of TGF-β 1 signaling which is the main factor that mediates fibroblast proliferation and extracellular matrix ...
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Promoter Methylation and Gene Expression in Human CD34+ Stem Cells Derived Erythroid Lineage by MicroRNA
... purified CD34+ cells derived from cord blood, the cells dispensed into 24 well plates and cultured at a density of 2×105 for seven days in incubator at 37°C with 5% ...was Stem Span ...
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Safety and efficacy of ex vivo expanded CD34+ stem cells in murine and primate models
... human CD34 + cells, and the difficulty in obtaining primate UCB, we use autologous primate mPB CD34 + cells for ...transplantation. CD34 + cells from non- human primates were ...
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The Effect of Granulocyte Colony Stimulating Factors (GCSF) on Expansion of CD34+ Hematopoietic Stem Cells
... (HSC) are clinically efficiently used for blood cancer therapy. More research indicates other uses of HSC for non-hematopoietic tissue such as for musculoskeletal tissue especially for ischemic chronic therapy in legs ...
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TOWARDS SUCCESSFUL ADAPTATION OF PLASMODIUM KNOWLESI TO LONG TERM IN VITRO CULTURE IN HUMAN ERYTHROCYTES
... Adaptation of P. knowlesi to Continuous in-vitro Culture in Human Erythrocytes: Many attempts have been made to adapt P. knowlesi to stably proliferate in human RBCs in-vitro. However, due to its low replication rates, ...
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Wharton’s jelly mesenchymal stem cell-based or umbilical vein endothelial cell-based serum-free coculture with cytokines supports the ex vivo expansion/maintenance of cord blood hematopoietic stem/progenitor cells
... the stem cell function of UCB-HSPCs ex vivo in the existence of additional ...hematopoietic stem cells is replantation in a NOD/SCID mouse transplant model, the LTC-ICs are means of the earliest ...
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Chimeric antigen receptor–induced BCL11B suppression propagates NK like cell development
... In general, low-cost production methods of approved agents have to compensate for investments during early phases of drug development. This paradigm has been profoundly challenged by the appearance of clinically highly ...
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Immunoprofiling of leukemic stem cells CD34+/CD38−/CD123+ delineate FLT3/ITD-positive clones
... leukemia-initiating cells called leukemic stem cells (LSCs) capable of propagating leukemia that are enriched in the CD34+/CD38 − ...normal CD34+/CD38 − hematopoietic stem ...by ...
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Circulating hematopoietic stem cell count is a valuable predictor of prematurity complications in preterm newborns
... Clearly, the question arises as to whether or not a number of SCs circulating in CB is associated with the development of premature birth complications. As a first step towards addressing this issue, we sought to ...
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The downregulated membrane expression of CD18 in CD34+ cells defines a primitive population of human hematopoietic stem cells
... 5 CD34 + cells were transplanted into NSG mice by retro-orbital ...BM cells the engraftment and lineage distribution by FACS, using hCD45 (#304014, BioLegend), hCD19 (#25-0198, eBioscience), hCD33 ...
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Ex-vivo expansion of nonhuman primate CD34+ cells by stem cell factor Sall4B
... hematopoietic stem/progenitor cells (HSPCs) was able to dramatically expand and enhance their ability for long-term engraftment in nonobese dia- betic/severe combined immunodeficiency (NOD/SCID) mice [1, ...
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Erythrocyte depletion from bone marrow: performance evaluation after 50 clinical scale depletions with Spectra Optia BMC
... apheresis variables on Spectra Optia BMC can have sig- nificant effects on product properties. However, we oper- ated with default settings for inlet flow, packing factor and collection flow and only adjusted collection ...
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BM microenvironmental protection of CML cells from imatinib through Stat5/NF-κB signaling and reversal by Wogonin
... BEL7402/5-FU cells to 5-FU via downregulation of P-glycoprotein expression ...MCF-7/DOX cells by inhibiting Nrf2 ...CML cells in BM microenvironment, and may be an attractive drug by targeting Stat5 ...
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Homing of HSCs From Their Niche by Honey And Bovine Colostrum For Treatment Of Induced Infertility In Male Mice
... of cells with markedly different destinies—one subpopulation of spermatogonia are presumably identical to their progenitors and continue to function as stem cells, the majority of spermatogonia enter ...
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Marking and Gene Expression by a Lentivirus Vector in Transplanted Human and Nonhuman Primate CD34+Cells
... Recently, gene delivery vectors based on human immunodeficiency virus (HIV) have been developed as an alternative mode of gene delivery. These vectors have a number of advantages, particularly in regard to the ability to ...
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Weidl, Eliane (2007): Die Transplantation von humanen endothelialen und angiogenen Zellen verbessert die linksventrikuläre Funktion im Myokardinfarktmodell der Nacktratte. Dissertation, LMU München: Tierärztliche Fakultät
... Nachdem ASAHARA 1997 berichtete, dass im peripheren Blut endotheliale Vorläu- ferzellen zirkulieren und diese in der Lage sind die Neovaskularisierung in einem Modell der Hintergliedmaßenischämie bei der Maus zu ...
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Mesenchymal stem cells promote a primitive phenotype CD34+c-kit+ in human cord blood-derived hematopoietic stem cells during ex vivo expansion
... their CD34+CD38- phenotype or slight modifications thereof [16, ...in CD34+CD38-Lin- ...CD133+, CD34+CD38-CD133+ or CD34+CD38- cells [6, 18, 19, ...that CD34+CD38- is the most ...
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Gene correction of HBB mutations in CD34+ hematopoietic stem cells using Cas9 mRNA and ssODN donors
... gene-corrected CD34 + HSCs and cloned into pJET1.2 vector using CloneJET PCR Cloning kit (https://www.thermo- fisher.com) and transformed into Top10 competent cells using standard cloning techniques. ...
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Original Article Smooth muscles and stem cells of embryonic guts express KIT, PDGFRRA, CD34 and many other stem cell antigens: suggestion that GIST arise from smooth muscles and gut stem cells
... In the present series, GIST, frequent expressed KIT (100%, 30/30), CD34 (90%, 27/30), and PDGFRA (83%, 25/30) were seen. In general, characteristics of tumors recapitulate their embryonic life. Therefore, it is ...
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An improved pre-clinical patient-derived liquid xenograft mouse model for acute myeloid leukemia
... of CD34 + and CD117 + cells remain unchanged between un- treated and treated mice (Additional file 6: Figure S6), sig- nificant reduction of absolute AML count was observed in the peripheral blood, spleen ...
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