cell based assay
A T cell based assay using specific Mycobacterium tuberculosis antigens, ESAT-6 and CFP 10, for the diagnosis of Tuberculous Meningitis.
... Culture for Mycobacterium tuberculosis is considered the gold standard in the diagnosis of tuberculosis [31]. Decontamination procedures needed for pulmonary, urinary and gastric juice sample is unnecessary for CSF and ...
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PubMedCentral-PMC5336325.pdf
... a cell-based assay of tyrosine phosphorylated MerTK (pMerTK), we used a 384-well format enzyme-linked immunosorbent assay (ELISA), 17 in which HEK293 cells are transfected with a cDNA encoding ...
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Real time detection of peptide–MHC dissociation reveals that improvement of primary MHC-binding residues can have a minimal, or no, effect on stability
... comparison with a more widely used cell-based assay. We found that data generated from both assays were largely in agreement, but the SPR technique appeared superior in detecting subtle differences ...
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Anti-Wolbachia drug discovery and development: safe macrofilaricides for onchocerciasis and lymphatic filariasis
... Wolbachia cell-based assay as the primary in vitro drug-screening ...validated assay, which has been adapted to automated high through- put-screening and represents a rapid, sensitive and e ffi ...
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Regulation of Herpes Simplex Virus Glycoprotein-Induced Cascade of Events Governing Cell-Cell Fusion
... a cell that ex- pressed the complementary reporter gene (positive signal) or a cell that did not (no ...ase assay were comparable in the presence of receptor (26, 27, 42, 50, 54), we quantified ...
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Rapid Detection of Cell Free Mycobacterium tuberculosis DNA in Tuberculous Pleural Effusion
... Other assays for the immunochemical biomarkers mostly reported in PE are the adenosine deaminase assay (ADA), the interferon gamma (IFN- ␥ ) assay, and IFN- ␥ release assays (IGRAs). Adenosine deaminase and ...
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A secondary RET mutation in the activation loop conferring resistance to vandetanib
... b Cell growth assay using IL3-independent Ba/F3 cells carrying lentivirally transduced CCDC6-RET cDNA with or without the S904F ...h, cell viability was measured using the CellTiter-Glo luminescent ...
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Mutations in Pseudorabies Virus Glycoproteins gB, gD, and gH Functionally Compensate for the Absence of gL
... limited cell-to-cell spread in the absence of ...serial cell culture ...fusion assay, two amino acid substitutions in the N-terminal part of gH ...
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Dual Split Protein-Based Fusion Assay Reveals that Mutations to Herpes Simplex Virus (HSV) Glycoprotein gB Alter the Kinetics of Cell-Cell Fusion Induced by HSV Entry Glycoproteins
... A model for prefusion gB provides a basis for understanding the complex kinetics of gB FR3 mutants. Uchida et al. (34) point out that structural domain III of gB is predicted by models of prefusion EBV gB to undergo ...
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MicroRNA-424 inhibits Akt3/E2F3 axis and tumor growth in hepatocellular carcinoma
... In present study, we profiled miRNA expressions by analysis of 840 mammalian miRNAs in hepatocellular carcinoma from 30 HCC samples. miR-424 was identified to be significantly down-regulated in HCC tissues as compared ...
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Evaluation of two high-throughput proteomic technologies for plasma biomarker discovery in immunotherapy-treated melanoma patients
... In this study, we compared two proteomic techniques widely used in biomarker discovery. We found that the Discovery and SOMAscan assays showed poor correl- ation in fluorescence data for 49 shared proteins, and as such, ...
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Multiplex detection in tonsillar tissue of all known human polyomaviruses
... An assay has earlier been set up for the detection of 10 HPyVs [52, ...acid assay for the detection of all 13 HPyVs currently known, in a clinically applicable ...bead based hybridization and flow ...
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High brain acid soluble protein 1(BASP1) is a poor prognostic factor for cervical cancer and promotes tumor growth
... cancer cell lines and in two papil- lomavirus-immortalized normal cervical cell ...cancer cell lines ...MTT assay indicated that overex- pression of BASP1 promoted cervical cancer cell ...
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<p>The Anti-Breast Cancer Effect and Mechanism of Glimepiride-Metformin Adduct</p>
... cancer cell proliferation by regulating the expression of non- coding ...cancer cell lines MDA-MB-231, MCF-7, CAL-148, and ...cancer cell lines, CAL-148 and MDA-MB-453 cells were more sensitive to ...
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Original Article Effect of CtBP2 silencing on proliferation, autophagy and EMT of non-small cell lung cancer
... cancer cell proliferation and EMT are important factors leading to metastasis and ...non-small cell lung cancer (NSCLC) remains ...epithelial cell line 16HBE cells were cultured in ...of cell ...
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Progressive Truncations C Terminal to the Membrane-Spanning Domain of Simian Immunodeficiency Virus Env Reduce Fusogenicity and Increase Concentration Dependence of Env for Fusion
... fusion assay that required both lipid and content mixing (syncytium assay) ...fluorescence assay to study the fusion process mediated by the SIV TM truncation mutants (TM185 to ...fluorescence ...
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Measurement of T-Lymphocyte Responses in Whole-Blood Cultures Using Newly Synthesized DNA and ATP
... As immune reconstitution becomes more important in the assessment of disease management and as cytokine thera- pies become available, there will be an increasing need to measure cellular immune function more rapidly. The ...
5
MicroRNA-382 inhibits cell growth and migration in colorectal cancer by targeting SP1
... carcinoma cell lines to promote cell proliferation and cell cycle progression ...various cell behav- iors including cell cycle, proliferation, and apoptosis [5, 11, ...osteosarcoma ...
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Comparison of a Classical Phagocytosis Assay and a Flow Cytometry Assay for Assessment of the Phagocytic Capacity of Sera from Adults Vaccinated with a Pneumococcal Conjugate Vaccine
... phagocytosis assay for pneumococci would be a great asset for the evaluation of the potential efficacy of (experimental) pneumococcal ...an assay could replace the laborious phagocytosis assay of ...
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Anticancer activity of 7 epiclusianone, a benzophenone from Garcinia brasiliensis, in glioblastoma
... the cell-cycle in G1-phase and induces apoptosis in colon cancer cell lines ...decrease cell viability and induce apoptosis of cancer cells [29, ...cancer cell lines, suggesting a possible ...
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