Cell culture and isolation of plasmid DNA
Isolation of genomic and plasmid dna of selected phenol degrading bacteria from industrial effluent treatment plants
... and plasmid DNA from selected potent phenol degrading bacteria from the effluent treatment plants of textile and trochemical ...the isolation of genomic and plasmid ...The plasmid ...
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Rapid procedure for isolation of plasmid DNA and application to epidemiological analysis
... Correlation of molecular weights to relative mobilities for seven plasmid DNAs of known molecular weights extracted from Escherichia coli K-12 C600. The relative mobilities of the other [r] ...
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Rapid plasmid DNA isolation from mucoid gram negative bacteria
... Plasmids prepared by the new method were highly soluble in conventional buffers and exhibited high-resolution plasmid banding patterns in agarose gels.. Plasmids as large as 180 kbp coul[r] ...
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Isolation and differentiation of herpes simplex virus and Trichomonas vaginalis in cell culture
... rly (15 to 18 h), it is often possible to reach a in vitro nor the growth of trichomonads on cell monolayers without having to wait for more extensive and in the absence of serum has bee[r] ...
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Direct isolation in cell culture of human rotaviruses and their characterization into four serotypes
... Approximately 10 to 100 50%o tissue culture infective doses of trypsin-treated virus were incubated with 10-fold dilutions of inactivated hyper- immune antisera before inoculation into o[r] ...
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Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids
... The choice of exosome marker for immobilization is a key issue. For MHC Class II– positive cells, the use of beads precoated with a pan class II–specific antibody is ideal. However, this marker is unsuitable for exosome ...
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Isolation of the equine granulocytic ehrlichiosis agent, Ehrlichia equi, in tick cell culture
... a cell line, IDE8, derived from a putative vector, the tick Ixodes ...tick cell culture medium with NaHCO 3 and an organic buffer [3-(N- morpholino)-propanesulfonic acid] ...Giemsa-stained ...
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First Isolation of Cytopathogenic Bovine Torovirus in Cell Culture from a Calf with Diarrhea
... Virus isolation in HRT-18 cells. At the second passage, CPE appeared in HRT-18 cells inoculated with the ileum contents of the dead calf. CPE characterized by the enlargement of cells were observed 2 to 3 days ...
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PLASMID ISOLATION FROM BACTERIA
... scale plasmid isolation leading to “crude lysates”, the quality of which being sufficient for analytical purposes, mainly agarose gel ...of plasmid DNA and therefore, additional ...
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Plasmid DNA purification
... The cell culture should be incubated at 37 °C with constant shaking (200–250 rpm) preferably 12–16 h over ...higher cell masses can be ...more plasmid DNA. Overgrowing a culture ...
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Alkaline Lysis Method Of Plasmid Isolation Protocol
... method. Isolation of plasmid DNA from E coli using the alkaline lysis method modified from Birnboim et al 1979 This protocol is suitable for fast cheap recovery of ...Your dna by an obstacle ...
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Bench Guide. Dear Customer, Genomic DNA. Plasmid DNA
... Erythrocytes (red blood cells) from birds, fish, and frogs contain nuclei and hence genomic DNA, while those from mammals do not. Since healthy mammalian blood contains approximately 1000 times more erythrocytes ...
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Encapsulation of plasmid DNA in calcium phosphate nanoparticles: stem cell uptake and gene transfer efficiency
... of plasmid DNA in calcium phosphate nanoparticles maintenance of the integrity of the plasmid encapsulated in the calcium phosphate nanocomposite ...encapsulated DNA intact during the entire ...
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Prawn DNA Isolation Procedure
... primary cell culture is so difficult to initiate and maintain for virus isolation purposes, bioassay should be the primary means for virus propagation ...
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Genomic Dna Isolation Protocol
... genomic dna isolation schemes have also available samples to sample! Is slow as these may require you to the ...genomic isolation protocol, without disturbing the cap with the ...control ...
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Purification of Plasmid DNA
... coli culture via overnight suspension. Inoculate the culture with a cell mass scraped from one colony selected from the +LB/amp plate from our transformation ...the culture 2-3 days in advance ...
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Effects of the presence of ColE1 plasmid DNA in Escherichia coli on the host cell metabolism
... ml culture broth by centrifugation at 10,000 g for 10 min, washed twice with TE buffer (100 mM Tris-HCl, pH ...crude cell extracts were immediately used for determination of spe- cific enzyme activities or ...
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Isolation and continuous culture of Neorickettsia helminthoeca in a macrophage cell line
... pathogenic to the canine species, three naive dogs were fed metacercaria-encysted kidneys of salmon caught in a river where infection of metacercariae with Neorickettsia helminthoeca has[r] ...
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Constructing and Characterizing a Recombinant DNA Plasmid
... desired DNA frag- ment or gene sequence can be identified, excised from the chromosome, isolated, and cloned into a number of different plasmids or ...The plasmid carrying the gene of in- terest can be ...
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LAB 11 PLASMID DNA MINIPREP
... of plasmid DNA using gel electrophoresis ...Solution. Cell Lysis Solution, which contains sodium dodecyl sulfate (SDS) and sodium hydroxide, is then ...denatures cell proteins, but ...
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