Cell Cycle (Eukaryotic), Genetic Regulation of
Models in biology: lessons from modeling regulation of the eukaryotic cell cycle
... studying cell growth and division in fission yeast by genetic tools, had discov- ered three genes involved in regulating entry into mi- tosis: cdc2 , cdc25 and wee1 ...The genetic evidence suggested ...
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Aberrant Cell Cycle Regulation in Cervical Carcinoma
... normal cell cycle control ...alter cell cycle checkpoints, thereby leading to the accumu- lation and transmission of genetic ...the regulation of growth ...
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Generation of Model Systems for the Study of Novel Cell Cycle Regulation in Development: Implications for Spy1 in Tumour Susceptibility
... digested to isolate the transgene fragment. The transgene is injected into a fertilized oocyte during the pronuclear period (Brinster et al., 1985; Si-Hoe et al., 2001). This is the period immediately following ...
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Role of Cks85A in cell cycle regulation
... Cdk1 binds to Cyclin A in G2 to phosphorylate Cdt1 in mammalian cells. Phosphorylated Cdt1 is ubiquitinated by SCF Skp2 and then degraded by the proteasome (Li et al, 2003). It has been shown that Cks85A interacts with ...
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Regulation of male germ cell cycle arrest and differentiation by DND1 is modulated by genetic background
... Quantitative RT-PCR (qRT-PCR) was used to determine relative expression levels of transcripts in XY gonads of different genotypes and strain backgrounds. Gonad dissection, RNA extraction and cDNA synthesis have all been ...
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Transcriptional Coactivator CBP Facilitates Transcription Initiation and Reinitiation of HTLV-I and Cyclin D2 Promoter
... average, 5- to 10-fold higher than in normal resting B lymphocytes [36]. Cyclin D3 was not detected in any sample from B-CLL or LPL patients, whereas cyclin D1 was expressed only in the 3 cases (LPL, 1; MCL, 2) ...
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“Natural Site Directed Mutagenesis” Might Exist in Eukaryotic Cells
... Site-directed mutagenesis refers to a molecular biology method that is used to make genetic alterations in the DNA sequence of the gene of interest [1] [2]. It is also called oligonucleotide-directed mutagenesis ...
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The ‘ORC’hestral symphony in dna replication across the species
... The S.cerevisiae ORC (ScORC) forms a stable hetero- hexameric complex binding to the specific DNA sequences throughout the cell cycle (Gibson et al., 2006). ScOrc1 to ScOrc5 contain a predicted AAA+ domain ...
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Studies on regulation of the cell cycle in fission yeast.
... To understand how biological processes, such as maintaining proper chromosome segregation function, it is necessary to perform detailed analysis on molecular basis with the help of microscopic visualisation. Modern ...
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Outlier Identification using Nonmetric Multidimensional Scaling of Yeast Cell Cycle Phase using Gene Expression Data
... the cell and gene expression analysis results are highly dependent on basic informations about samples and not all available time series gene ex- pression data include these ...yeast cell cycle ...
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The Complementarity Effect for Cdc25 Phosphatase Inhibitors
... of eukaryotic cell cycle by activating cyclin Cdk that serve as central regulators of cell cycle with the lead role of each state of cell ...to cell cycle ...
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Suppression of STIM1 inhibits human glioblastoma cell proliferation and induces G0/G1 phase arrest
... glioblastomas cell of different transformation degree, especially higher expressed in U251 cells that were derived from a high-grade glioblast- oma; therefore, these phenomenon represent a reason- able cell ...
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When cell cycle meets development
... on cell size ...a cell can be measured accurately and, when compared with cell cycle parameters, has enabled for the first time a clear understanding of how cell size changes through ...
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Index Catalog // Carolina Digital Repository
... The initial screen yielded interesting and instructive findings. As expected, this approach confirmed previous studies showing that origins tend to be depleted for nucleosomes (Fig 3.1 A). While origins are like ...
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Expression of human eukaryotic initiation factor 3f oscillates with cell cycle in A549 cells and is essential for cell viability
... similar genetic organization as that in most organ- isms (data not shown), while the intronless one has char- acteristics of a retrocopy [15,27]; both sequences having a 97% identity between ...human cell ...
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Autophagy in the Eukaryotic Cell
... Even if the basic machinery for autophagy is identical be- tween yeast and mammalian cells, the possibility that this phe- nomenon is more complex in higher eukaryotes cannot be excluded. For example, in addition to LC3, ...
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Regulation of the Cell Division Cycle in Trypanosoma brucei
... Chromosome segregation is driven by microtubule bundles, which are termed kinetochore fibers, in the bipolar mitotic spin- dle. In animals, spindle microtubules are nucleated either by mi- crotubule organizing centers ...
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Gassen, Alwine (2012): Functional analysis of DOT1-dependent histone H3 lysine 76 methylation during cell cycle progression in Trypanosoma brucei. Dissertation, LMU München: Fakultät für Biologie
... Chromatin immunoprecipitation was applied as published by Siegel et al. using magnetic beads coupled to anti-rabbit IgG antibodies (Siegel et al., 2009). 100 µl beads were washed with 1 ml of block solution and ...
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EUKARYOTIC CELL
... cRNA (complementary RNA); RNase (ribonuclease); DNase (deoxyribonuclease); rRNA (ribosomal RNA); mRNA (messenger RNA); tRNA (transfer RNA); AMP, ADP, ATP, dAMP, ddATP, and GTP, etc. (for the respective 5 ⬘ phosphates of ...
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EUKARYOTIC CELL
... nomes. Genetic symbols may be one, two, or three let- ...the genetic symbols and designations for such inserted DNA should conform to those used for the host ...
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